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Method for detecting pre-treatment of nitrofurans metabolite

A technology of nitrofuran and detection method, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of single, only one of them can be detected at a time, cumbersome and time-consuming extraction operations, expensive detection reagents, etc., so as to simplify extraction. and the effects of derived manipulation, low detection cost, and high sensitivity

Inactive Publication Date: 2011-01-19
北京六角体科技发展有限公司
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Problems solved by technology

The pretreatment time of liquid chromatography-tandem mass spectrometry requires 16 hours, and the operation is cumbersome and time-consuming; the pretreatment time of ELISA requires 16 hours, and the detection method is single, and only one of them can be detected at a time. High, the extraction operation is cumbersome and time-consuming

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  • Method for detecting pre-treatment of nitrofurans metabolite
  • Method for detecting pre-treatment of nitrofurans metabolite

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Embodiment Construction

[0016] Below, the present invention will be described in further detail in conjunction with the accompanying drawings.

[0017] First, the sample sampling process for obtaining samples is carried out. In this sampling step, fish, scales and skins are removed, and muscles are taken along the back; shrimp, heads and shells are removed, and muscle parts are taken; crabs, soft-shelled turtles, etc. are taken for edible parts , the sample was cut into small pieces, crushed with a beater, mixed homogeneously, and set aside.

[0018] Next, mix the sample with the extractant evenly and obtain the supernatant; then add the extractant for re-extraction and obtain a mixed extract. Weigh 10g of the crushed sample (accurate to 0.01g), put it into a 50mL centrifuge tube, add extractant 1 and 10mL extractant 2 successively, vortex and mix, shake or ultrasonically shake at 40°C for 30min, and place at 6000r / Centrifuge for 10 min; take out the supernatant, then add 10 mL of extractant 2, rep...

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Abstract

The invention relates to a method for detecting pre-treatment of nitrofurans metabolite, comprising the following steps: (1) choosing a sample; (2) uniformly mixing the sample and extractant to obtain supernate; (3) adding the extractant to carry out extraction once again and obtaining mixed extractant; (4) adding derivatization reagent and catalyst to the mixed extractant and uniformly mixing the mixture; (5) cooling the mixture to room temperature; (6) adjusting the mixed extractant to neutrality and adding ethyl acetate to carry out extraction; (7) repeating the operation and combining ethyl acetate layers; (8) carrying out rotary evaporation on the mixture to be dry and adding methanol solution to dissolve residue; (9) activating a purification pillar to cause the methanol solution to pass through the purification pillar and obtaining and drying eluent; (10) adding acetonitrile aqueous solution and isooctane to the residue, dissolving and obtaining lower acetonitrile water layer; (11) causing the acetonitrile water layer to pass through a microfiltration membrane; and (12) analyzing and measuring the components and contents of the nitrofurans metabolite. The method of the invention is accurate, reliable and highly sensitive and enjoys good technical effect.

Description

technical field [0001] The invention relates to a detection method, in particular to a pretreatment detection method of nitrofuran metabolites, belonging to the field of food safety detection. Background technique [0002] Nitrofurans are a class of synthetic antibacterial drugs, they act on microbial enzyme systems, inhibit acetyl-CoA, interfere with the metabolism of microbial sugars, and thus play an antibacterial role. Because of its low price and good curative effect, it is widely used in livestock and poultry and aquaculture fish to treat enteritis caused by Escherichia coli or Salmonella, scabies and ulcers in farmed fish, and can be added to feed or aquaculture medicine bath use. Since nitrofuran antibacterial agents and their metabolites have carcinogenic, teratogenic and safety concerns when ingested by the human body for a long time, the European Union (European Union) has banned nitrofuran antibacterial agents in edible livestock, poultry and aquatic animals sin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 贾东芬王亚军
Owner 北京六角体科技发展有限公司
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