An indirect ELISA kit for the detection of serotype 8 avian adenovirus antibodies based on spike protein f
A technology of poultry adenovirus and spike protein, which is applied in the field of biotechnology detection and achieves good specificity
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Embodiment 1
[0041] The indirect ELISA kit of the present invention can be obtained through the following steps:
[0042] (1) Isolation of FAdV-8 avian adenovirus:
[0043] Take (suspected) liver of dead chicken infected by avian adenovirus, grind it and add PBST at a ratio of 1:5 to make a suspension; centrifuge at 5000r / min for 15min, take the supernatant; ml, react at 37°C for 30 minutes; filter through a 0.45um microporous filter, inoculate 8-day-old SPF chicken embryos through the allantoic cavity at a dose of 0.2ml / embryo, and collect allantoic fluid 96-120 hours after inoculation; allantoic fluid After the solution was identified as FAdV-8 avian adenovirus, it was stored at -20°C.
[0044] (2) Preparation of avian adenovirus genome:
[0045] Get 400uL supernatant of FAdV-8 isolated strain virus (serum type 8 avian adenovirus) in a 1.5mL finger tube, add 400uL cell lysate (50mmol / LTris-HCl pH 8.0, 20mmol / L EDTA pH 8.0, 2% SDS and Proteinase K), mix well and place in a water bath a...
Embodiment 2
[0060] Infection sample detection effect
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