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Colloidal gold test strip for detecting chicken infectious anemia virus

A chicken infectious anemia, colloidal gold test paper technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of inconvenient use of kits, environmental pollution of silver nitrate, etc., achieve stable results and low testing costs , highly specific effect

Inactive Publication Date: 2015-06-24
孙素玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ELISA rapid detection kit of chicken infectious anemia virus antibody has been developed at home and abroad, this kit is to detect chicken infectious anemia virus antibody in serum, but in the operation process with this diagnostic kit, some reagent On-site preparation is required, and the use of this kit is also inconvenient
Domestic Liang Zhixuan has carried out the detection method of chicken infectious anemia virus antibody dot immune colloidal gold infiltration test, which is the detection of chicken infectious anemia virus antibody in serum, but there is a danger of silver nitrate to the environment
So far, there has been no report on the establishment of a colloidal gold rapid detection test strip method for chicken infectious anemia virus

Method used

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  • Colloidal gold test strip for detecting chicken infectious anemia virus
  • Colloidal gold test strip for detecting chicken infectious anemia virus
  • Colloidal gold test strip for detecting chicken infectious anemia virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of chicken infectious anemia virus detection colloidal gold test strip is prepared by the following method:

[0031] 1. Preparation, purification and detection of chicken anti-CIV antibody

[0032] 1. Preparation of chicken anti-CIV antibody

[0033] SPF chickens were immunized 4 times with chicken CIV attenuated vaccine (German Roman Animal Health Co., Ltd.), blood was collected, and serum antibodies were prepared.

[0034] 2. Purification of Chicken Anti-CIV Serum Antibody

[0035] Serum antibodies were purified twice by caprylic acid-saturated ammonium sulfate method and antigen purification antibody method.

[0036] Conventional octanoic acid-saturated ammonium sulfate method:

[0037]Take 3ml of serum, add 6mL of 0.06M pH4.8 acetate buffer, stir evenly, add 225μL octanoic acid while stirring at room temperature, stir for 30min, and stand at 4°C for 2h. Centrifuge the serum solution at 800 rpm at 4°C for 30 min, take the supernatant, and adjust the pH to ...

Embodiment 2

[0083] Embodiment 2 test strip detection

[0084] 1) Specificity test of test strips

[0085] Add 2 drops of CIV, AIV, NDV, and MDV virus solutions to the sample pad of the test strip, and observe after 10 minutes. image 3 As shown in the results, the detection line and the quality control line of the test strips dripping with CIV virus liquid are all red; the test strips with AIV, NDV, and MDV virus liquids are all only the quality control line is red, and the detection line is not. red. It shows that this colloidal gold test strip does not cross-react with AIV, NDV, MDV virus.

[0086] 2) Sensitivity test of test strips

[0087] Add 2 drops of virus solution containing 10, 1, 0.1, 0.01 μg / mL CIV on the test strip sample pad respectively, observe after 10 minutes, as Figure 4 As shown, the results show that the detection line develops a good color when the CIV is 10.1 μg / mL, the detection line still develops color when the CIV is 0.1 μg / mL, but it is not too clear, and ...

Embodiment 3

[0094] Example 3 Application of chicken infectious anemia virus detection colloidal gold test strip

[0095] 1. Artificial inoculation of CIV to prepare sick chicken model

[0096] Pathogenic chicken infectious anemia virus (isolated by the poultry disease prevention and control laboratory of Anhui University of Science and Technology, named Anhui-2011-1) solution and EP tube, treated in a 70°C water bath for 30 minutes, and then added 50% Ether was mixed and treated for 1 hour, and the treatment solution was inoculated subcutaneously on the neck of 1-day-old SPF chicks, 0.5 mL each, and subcutaneously injected to infect 8 1-day-old SPF chickens, and 8 chickens that were not injected were used as controls. After 14 days of infection by injection, symptoms such as pale cockscomb, growth retardation, depression, emaciation, wing dermatitis, and punctate hemorrhage appeared in the limbs. Dissection found that the blood was thin, the coagulation time was prolonged, and the change...

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Abstract

The invention discloses a colloidal gold test strip for detecting chicken infectious anemia virus. The colloidal gold test strip comprises a chicken anti-CIV (Huma Certain Immunodeficiency Virus) gold-labeled antibody glass fiber film, a nitrocellulose film, a water absorbing cushion and a PVC (polyvinyl chloride) bottom plate, wherein the nitrocellulose film is adhered to the center of the PVC bottom plate; the glass fiber film is formed by overlapping and connecting a sample cushion and a combining cushion; the other end of the combining cushion is adhered to one end with a T line, of the nitrocellulose film; the water absorbing cushion is adhered to one end with a C line, of the nitrocellulose film. With the adoption of the test strip for detecting, the sample is simply dropwise added onto the sample cushion of the test strip, and the operation is fast, simple and convenient, can be performed by one step, and is free of any instrument; the test strip is suitable for on-site use.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a colloidal gold test strip for chicken infectious anemia virus detection. Background technique [0002] Chicken infectious anemia (CIA) is an immunosuppressive infectious disease characterized by aplastic anemia, systemic lymphoid tissue atrophy, and subcutaneous muscle hemorrhage in chicks caused by chicken infectious anemia virus (CIV). CIA caused increased mortality, decreased survival and body weight gain in flocks. Severe immunosuppression is caused by immune organ damage, which reduces the resistance of chickens, increases the susceptibility of chickens to various pathogens, reduces the effect of chickens on various conventional vaccines, and brings great benefits to the prevention and treatment of other diseases. Difficulty, can cause secondary infection at the same time, bring very big loss to poultry industry. Since CIA was first reported by Yuasa ...

Claims

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Application Information

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IPC IPC(8): G01N33/96
CPCG01N33/56983
Inventor 孙素玲张训海赵磊
Owner 孙素玲
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