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Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid

A sesquiterpene and fermentation liquid technology, applied in the field of biological fermentation and medicine, can solve the problem of no secondary metabolite sesquiterpenoids

Inactive Publication Date: 2008-07-23
章克昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the medical efficacy of golden ear, some scholars have carried out in-depth research on golden ear from nutritional factors such as culture medium, culture conditions and polysaccharides, but there is no report on secondary metabolites and sesquiterpenoids

Method used

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  • Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Tissue isolation of aureus species

[0056] The fresh unopened fruit body of Yunnan wild golden ear was taken, the base of the stipe and the outer epidermis of the stipe were cut off, and the surface of the fruit body was disinfected with 75% ethanol, then transferred to an ultra-clean workbench and irradiated with ultraviolet light for 20 min. After burning the scalpel on the flame of an alcohol lamp, put it in sterile water to cool down to ensure that the temperature of the scalpel will not damage the fruiting body tissue. Divide the fruiting body into two, cut the inner tissue of the epidermis in the stipe (or in the stipe), the junction of the stipe and the cap, the cap and the gill, the small pieces the size of soybean, cannot cut through the surface of the fruiting body when cutting organize. Use sterile tweezers to connect the test tube slant medium (that is, PDA medium), connect 10 pieces of each tissue, cultivate in the dark at 25-28 °C, and observe...

Embodiment 2

[0057] The preparation of embodiment 2 golden ear fermented liquid

[0058] 1. In the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microorganism Experiment Technology Manual, 1994: P 367), the golden ear strain obtained in Example 1 was inserted, and the culture temperature was 27° C. until the mycelium grew. After the sloping surface is full, the slanted surface strains were inserted into a 250 mL conical flask (5 bottles in total) containing 60 mL of shaking flask medium, and cultured at 27° C., 150 rpm shaker for 24 hours. Wherein the formula of the shake flask culture medium is (unit is g / L): glucose 5, corn flour 15, soybean meal flour 5, KH 2 PO 4 3. MgSO 4 1. The initial pH was 6.3.

[0059]2. Insert the above-mentioned shaking flask strain into a 500 mL conical flask (24 bottles in total) containing 150 mL of expansion medium, and the inoculation amount is 11 mL of shaking flask seeds per 500 mL conical flask, and shake at 27°C and ...

Embodiment 3

[0063] The preparation of embodiment 3 sesquiterpene crude product

[0064] The acidity of the fermentation broth obtained in Example 2 was adjusted to pH 1.0, and after 30 minutes of centrifugation at 3000 rpm, the supernatant was adsorbed by non-polar macroporous resin adsorption AB-8, and the flow rate was 1 / 20 column volume / min. After the end, the resin column was washed with a large amount of water until colorless, eluted with 20% ethanol solution, the eluate was concentrated in vacuo, and the concentrated solution was freeze-dried to obtain a crude sesquiterpene product. The crude product is yellow or light yellow powder, dissolved in 40% methanol and ethanol aqueous solution, hot water, pyridine, and alkaline solution, and shaken in hot water to produce relatively lasting foam. It consists of:

[0065]

[0066] The dry weight of the finally obtained sesquiterpenoid composition was 2.8 g / L, and the inhibition rate of sesquiterpenoids at a concentration of 5 mg / mL on ...

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Abstract

The invention relates to a process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar, belonging to the biological fermentation and medicine field. The process flow is characterized in that the process flow applicant finds the fermentation liquid and the sesquiterpene produced by the production process are applied on a high blood sugar mouse through an oral administration route, the mouse significantly reduces the blood sugar and the fructosamine, which approves that the golden fungus fermentation liquid and the sesquiterpene are possessed of preparing a health care drink and medicine to reduce the blood sugar; the fermentation liquid color is orange to yellow-brown; the inhibitive rate to the ex vivo non-enzymic glycosylation is up to 72% to 90% with the fermentation liquid and 5mg / ml sesquiterpene. The process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar has the advantages of not only creating a fire-new channel to further develop the golden fungus but also providing an ideal Chinese herbal medicine to reduce blood sugar.

Description

technical field [0001] The present invention relates to the fields of biological fermentation and medicine. Specifically, the present invention relates to a method for producing auricularia fermented liquid or sesquiterpene by fermenting auricularia aureus, the auricium auricum fermented liquid or sesquiterpene crude product obtained by the method, and the fermented Liquid or sesquiterpene crude pharmaceutical composition and / or health care products. The auricularia fermented liquid or sesquiterpene crude product has multiple medicinal functions, and can be used to prepare medicines for preventing and / or treating diabetes, cardiovascular and cerebrovascular diseases or bronchitis, especially it has hypoglycemic effect, making it a Blood sugar provides ideal medicine. Background technique [0002] Diabetes is a common and frequently-occurring disease among middle-aged and elderly people. The complications caused by diabetes seriously endanger people's life and health. The e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P5/02A61K36/07A61P3/10
Inventor 章克昌张志才丁重阳顾正华王玉红
Owner 章克昌
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