Preparation of pesticide active matter in stellera chamejasme and use

A technology of Rhizoma Rhizoma Radix and Rhizoma Rhizoma Rhizoma Radix are applied in the field of pesticides, which can solve the problem of not specifying the purpose of the compound, and achieve the effects of saving manpower, complete extraction and good separation effect.

Inactive Publication Date: 2006-01-04
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[Feng Baomin, Pei Yuehu, Han Bing, "Study on the chemical constituents of Rhizoma chamaejasma", Journal of Shenyang Pharmaceutical University, 2000, 17(4)

Method used

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  • Preparation of pesticide active matter in stellera chamejasme and use
  • Preparation of pesticide active matter in stellera chamejasme and use
  • Preparation of pesticide active matter in stellera chamejasme and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation and contact activity determination of Daphne chamaejasme extract

[0030] Chamaejasma chamaejasma is collected from Ruoergai Grassland in Sichuan Province. The collected fresh chamaejasma roots are washed, dried, and pulverized with a grinder to obtain fluffy chamaejasma powder.

[0031] Weigh a certain amount of chamaejasma root dry powder, add 10 times the mass of absolute ethanol to soak, extract and concentrate, and then use different polar solvents n-hexane, chloroform, ethyl acetate, methanol for graded extraction to obtain different polar extracts , repeated 3 times until the extraction was complete, and the same extracts were combined.

[0032] According to the method introduced by Zhang Zongbing, the antifeedant activity and contact activity were used to measure the toxicity: first, the cabbage caterpillars were collected in the vegetable fields in the suburbs where no pesticides had been applied, and healthy third-instar individuals with ...

Embodiment 2

[0041] Embodiment 2 Chromatographic separation and purification of Daphne chamaejasme insecticide active substance

[0042] Thin-layer chromatography silica gel GF254 was used as the stationary phase of adsorption chromatography, and plates were plated on a thin-layer automatic plate plater according to conventional methods. Sheet size: 5×10(cm), silica gel thickness: 0.25mm. Put the laid boards into an oven at 105°C for half an hour, and store them in a desiccator after cooling for later use. Use a spotting capillary to spot a sample, use n-hexane, chloroform, ethyl acetate, dichloromethane, methanol and other solvents with different compositions and different proportions of mixed solvents as developing agents, and after developing, irradiate with 254nm ultraviolet analysis lamp and iodine Three methods of powder vapor and sulfuric acid-ethanol color development were combined to detect bands. Choose a solvent with good separation effect and no tailing for reference in colum...

Embodiment 3

[0061] Embodiment 3 active substance structure identification

[0062] Compound A

[0063] Compound A is a light yellow oily substance, easily soluble in organic solvents such as petroleum ether, chloroform, ethyl acetate, etc., and shows blue-purple spots on the thin layer of silica gel GF254 under the ultraviolet analysis lamp. Spectral data analysis is as follows:

[0064] Uvλmax (methanol) nm; 283.0, 309.0 (weak). The weak peak at 309.0nm of the ultraviolet absorption peak is n→π of (carbonyl) * Transition, UV absorption peak 283.0 is π→π * transition.

[0065] IR v max cm -1 : 3400.81 (hydroxyl, blunt peak), 3027.84 (benzene ring hydrogen), 2930.28, 2858.62 (saturated hydrogen), 1682.26 (carbonyl), 1598.67, 1581.49, 1496.20, 1450.86, 753.90, 699.65 (monosubstituted benzene ring).

[0066] 1 H-NMR (CDCl 3 , 600Hz) δppm: 1.90 (2H, dm) for 4-digit H, δ2.83 (2H, dm), for 5-digit H, δ3.13 (2H, M), for 2-digit H, δ3.95 (1H, m) is hydroxyl H, δ4.25 (1H, m) is 3-position...

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Abstract

The present invention discloses a kind of n-hexane extract of stellera chamaejasme and the application of its further separated active compounds A and B as pesticide. Bioactivity test with cabbage worm as target pest shows the n-hexane extract of stellera chamaejasme diluted in 30 times has 72 hr corrected cabbage worm death rate of 100 %, the compound A, 1, 5-diphenyl-3-hydroxy-1-pentanone, has contact killing activity on cabbage worm of 483.4 mg/L (LC50), and the compound B, 4-hydroxy-sesquiterpene-propylene-ester, has contact killing activity on cabbage worm of 530.3 mg/L(LC50). The present invention lays foundation for developing green pesticide with stellera chamaejasme.

Description

technical field [0001] The invention relates to the application of a daphne chamaejasme active substance in pesticides, belonging to the field of pesticides. Background technique [0002] Due to the long-term irrational use of chemical pesticides, many disadvantages have been brought about, such as environmental pollution, direct poisoning of non-target organisms, and easy resistance of pests. In recent years, with the implementation of IPM theory, the development of sustainable development strategies and the improvement of human health requirements, the research and development of biopesticides with good environmental compatibility, safety, low residue and economy have been paid more and more attention by people. gradually accepted by the market. Plant pesticides are becoming a research hotspot in the creation of new pesticides. [0003] Ruixiang wolfbane (Stellerachanlaejastne L.), red wolfbane, Mian Dajian, a stick of incense, red match head, heartbroken grass. Mongoli...

Claims

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Application Information

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IPC IPC(8): A01N37/02
Inventor 侯太平肖波张可君陈琳鲁韬
Owner SICHUAN UNIV
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