Sesquiterpene alcohol compound, preparation and application thereof

A technology of semiterpene alcohols and compounds, which is applied in the field of sesquiterpene alcohol compounds and their preparation, and can solve problems such as drug residues, environmental secondary pollution, and non-red tide biological hazards

Active Publication Date: 2018-08-31
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although chemicals such as copper sulfate and chlorine can quickly and effectively kill or inhibit harmful algae or inhibit their gro...

Method used

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  • Sesquiterpene alcohol compound, preparation and application thereof
  • Sesquiterpene alcohol compound, preparation and application thereof
  • Sesquiterpene alcohol compound, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The structure of the sesquiterpene alcohol compound derived from the epiphytic fungus of seaweed is shown in formula (I).

[0026]

[0027] The compound has the following physicochemical and spectroscopic properties:

[0028] Colorless oil; specific rotation [α] 20 D -29 (c0.13, MeOH); H NMR spectrum (solvent is deuterated chloroform) δ H 1.69(dd, 14.1, 8.1), 1.61(dd, 14.2, 3.3), 4.03(dd, 8.2, 3.3), 1.56(dd, 11.2, 1.4), 1.68(m), 1.57(m), 2.29(m) , 4.20(dd, 10.2, 5.7), 2.10(dd, 12.7, 5.7), 1.20(dd, 12.6, 10.5), 1.76(heptet, 6.9), 0.96(d, 6.9), 0.86(d, 6.9), 5.11 (br s), 4.91(br s), 1.16(s); C-NMR spectrum (solvent is deuterated chloroform) δ C 41.3(C), 51.0(CH 2 ), 72.6(CH), 84.0(C), 52.1(CH), 22.3(CH 2 ), 31.6 (CH 2 ), 153.7(C), 73.2(CH), 51.7(CH 2 ), 35.1 (CH), 17.3 (CH 3 ), 17.8 (CH 3 ), 111.9 (CH 2 ), 22.4 (CH 3 ); high-resolution mass spectrometry [M] + m / z 254.1889, calculated 254.1882.

Embodiment 2

[0030] The preparation method of the sesquiterpene alcohol compound as shown in formula (I):

[0031] Take Trichoderma virens (Trichoderma virens) Y13-3 strains that grow well on the plate, cut into small pieces and inoculate them in potato dextrose liquid medium, put 300 ml of medium in each 1 liter Erlenmeyer flask, 200 bottles in total, at room temperature Static fermentation for 30 days, then extracted three times with ethyl acetate, concentrated under reduced pressure to obtain 27.2 grams of crude extract after concentration.

[0032] The potato glucose liquid medium is composed of 500 milliliters of boiled juice containing 100 grams of potatoes per liter, 20 grams of glucose, 5 grams of peptone, 5 grams of yeast extract powder, and 500 milliliters of aged seawater.

[0033] Trichoderma virens (Trichoderma virens) Y13-3 strain was preserved in the China Type Culture Collection Center CCTCC on January 10, 2018, address: Wuhan University, China, the preservation number is C...

Embodiment 3

[0038] The difference from Example 2 is that

[0039] Take Trichoderma virens (Trichoderma virens) Y13-3 bacterial strain that grows well on the plate, cut into small pieces and inoculate in Jerusalem artichoke glucose liquid medium, put 300 ml of medium in each 1 liter Erlenmeyer flask, 100 bottles in total, room temperature Static fermentation for 40 days, filtration and collection of mycelia and fermentation broth respectively.

[0040] The Jerusalem artichoke glucose liquid medium consists of 500 milliliters of boiled juice containing 100 grams of Jerusalem artichoke tubers per liter, 20 grams of glucose, 5 grams of peptone, 5 grams of yeast extract powder, and 500 milliliters of aged seawater.

[0041]Collect about 30 liters of fermented liquid, extract three times with ethyl acetate, and concentrate under reduced pressure; the mycelium is dried and pulverized, extracted three times with ethyl acetate, and concentrated under reduced pressure; the concentrate is detected b...

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PUM

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Abstract

The invention relates to the field of algae inhibitors, the invention specifically relates to a sesquiterpene alcohol compound derived from algal epiphytic fungus, a preparation method thereof and anapplication of the sesquiterpene alcohol compound in the aspect of inhibiting microalgae. The specific structural formula is shown in the formula (I). The preparation method comprises the following steps: inoculating a fungal culture medium with Trichoderma virens Y13-3 for fermentation culture, and subjecting a fermentation product to separating and purifying, and the product is namely a sesquiterpene alcohol compound as shown in the formula (I). The median inhibition concentration of the sesquiterpene alcohol compound to the microalga can be up to 1.2 microgram/ml through the microalga activity inhibition experiment.

Description

technical field [0001] The invention relates to the field of algae inhibitors, in particular to a sesquiterpene alcohol compound derived from algal epiphytic fungi, a preparation method thereof and an application in inhibiting microalgae. Background technique [0002] Worldwide, coastal red tide disasters are becoming more and more serious, seriously hindering the development of marine fisheries, near-shore tourism and marine economy. Since 2000, my country has entered a period of high incidence of red tides, and the frequency and degree of damage of red tides have increased significantly. There are four main harmful forms of red tide, one is to destroy the balance of marine ecology; the other is to destroy marine fishery resources; the third is to endanger human health; the fourth is to affect the development of marine tourism. According to statistics, the annual economic losses caused by red tides are as high as more than 1 billion yuan. There are about 300 species of mi...

Claims

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Application Information

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IPC IPC(8): C07C35/34C12P7/02A01N31/06A01P13/00C12R1/885
CPCA01N31/06C07B2200/07C07C35/34C12P7/02C07C2602/30
Inventor 季乃云时振振
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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