Method for simultaneously detecting sesquiterpene and curcumin components

A technology for curcumin and sesquiterpenes, which is applied in the field of simultaneous detection of sesquiterpenes and curcuminoids, can solve the problems of not simultaneously measuring sesquiterpenes and curcuminoids and the like

Active Publication Date: 2013-06-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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Benefits of technology

The present inventors have developed an improved way for accurately measuring different types of medicine called cuminolene or currylanoid compounds (CurC). They found these molecules were able to bind with certain proteins on cells they are studying. By comparing this binding data between healthy tissues from patients who had taken them up by chemotherapy treatment, researchers discovered new ways to improve their effectiveness against cancer. These methods could lead to better understanding how drugs work through specific targeted pathways within tumor cell populations.

Problems solved by technology

This patented technical problem addressed by this patents relates to identifying specific molecules from different plants that can help with their health benefits like prevention against diseases caused by viruses or bacteria.

Method used

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  • Method for simultaneously detecting sesquiterpene and curcumin components
  • Method for simultaneously detecting sesquiterpene and curcumin components
  • Method for simultaneously detecting sesquiterpene and curcumin components

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Embodiment Construction

[0016] The method for simultaneous determination of sesquiterpenoids and curcuminoids components of the present invention is the optimal solution obtained through a large number of screening tests, in conjunction with specific embodiments, the present invention is further described as follows:

[0017] 1 Medicinal materials, instruments and reagents

[0018] Source of medicinal materials: Curcuma zedoary is purchased from the main producing areas of zedoary (Zhejiang, Guangxi, Sichuan), and identified as Curcuma phaeocaulis Valeton and Curcuma kwangsiensis S.G.Lee et C.F. Liang) or dried rhizome of Curcuma wenyujin Y·H·Chen et C·Ling. Crushed through a 60-mesh sieve for later use. See Table 1 for medicinal materials and production batch numbers:

[0019] Table 1 General situation of samples

[0020]

[0021] Agilent1200 high performance liquid chromatography (including online degassing system, binary gradient pump, autosampler, column thermostat, diode array detector), K...

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Abstract

The invention discloses a method for simultaneously detecting sesquiterpene and curcumin components, and especially relates to the method for simultaneously detecting sesquiterpene and curcumin components in curcuma rhizome, by using the method provided by the invention, five sesquiterpene components and three curcumin components in curcuma rhizome enable quantitative determination in a simple and reliable mode, simultaneous detection of two components is helpful for more comprehensive control of quality of curcuma rhizome, the detecting method respectively expands linear relation investigation, precision test, stability test, repeatability test and sample adding reclamation rate, and the result proves that the method is accurate and reliable.

Description

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Claims

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Application Information

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Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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