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Novel cryoprotective liquid

A cryoprotective solution and a new technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of sperm damage, reduce the conception rate and other problems, and achieve the effects of reducing toxicity, improving vitality and improving sperm quality

Pending Publication Date: 2021-03-19
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The cryopreservation of sperm can prolong its life in vitro, but due to the changes in the structure and physiological function of sperm during freezing and thawing, the sperm will be damaged, which will greatly reduce its pregnancy rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A new type of cryoprotectant solution, including: egg yolk 200mL / L, sodium citrate 13.536g / L, tris 24.224g / L, glucose 11g / L, streptomycin sulfate 1 million units 1g / L, penicillin 1.6 million units 0.8g / L, glycerin 30mL / L, trehalose 0.05moL / L.

Embodiment 2

[0015] A new type of cryoprotectant solution, including: egg yolk 200mL / L, sodium citrate 13.536g / L, tris 24.224g / L, glucose 11g / L, streptomycin sulfate 1 million units 1g / L, penicillin 1.6 million units 0.8g / L, glycerin 30mL / L, trehalose 0.1moL / L.

Embodiment 3

[0017] A new type of cryoprotectant solution, including: egg yolk 200mL / L, sodium citrate 13.536g / L, tris 24.224g / L, glucose 11g / L, streptomycin sulfate 1 million units 1g / L, penicillin 1.6 million units 0.8g / L, glycerin 30mL / L, trehalose 0.15moL / L.

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Abstract

The invention discloses a novel cryoprotective liquid, which comprises: 200mL / L of yolk, 13.536g / L of sodium citrate, 24.224g / L of trihydroxymethyl aminomethane, 11g / L of glucose, 1g / L of 1 million unit streptomycin sulfate, 0.8g / L of 1.6 million unit penicillin, 30mL / L of glycerin and 0.05moL / L-0.15moL / L of trehalose. According to the cryoprotective liquid, the toxicity of the protectant is reduced, and the freeze-thawing effect is improved; when sperms are frozen, the vitality, plasma membrane integrity, acrosome integrity, tyrosine phosphorylation level, protamine deficiency level, motilityrate and mitochondrial membrane potential of the sperms can be remarkably improved, and the quality of the frozen and thawed sperms is improved.

Description

technical field [0001] The invention relates to the technical field of cryopreservation, in particular to a novel cryoprotectant solution. Background technique [0002] Yanbian Yellow Cattle has excellent breed characteristics and meat characteristics, and has distinct regional characteristics. It is one of the five local fine breed cattle in China. The effects of cryopreservation on sperm function and fertility have been extensively studied, especially in cattle. Sperm cryopreservation is an effective way to preserve mammalian sperm. This method is often used to cultivate good offspring, the production potential of livestock, and artificial insemination for adjuvant treatment due to fertility impairment. Therefore, for genetics and breeding, sperm cryopreservation is a very important method. Viable sperm are removed from the male genitalia and processed during freezing to facilitate genetic improvement between species. The most important challenge in semen cryopreservat...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215A01N1/0221A01N1/0226
Inventor 金一吕艳秋陈璇罗晓彤韩越程咪咪曲星霖张雨阳孔令敏李春宇尹伊
Owner YANBIAN UNIV