Disclosed is a Chinese traditional medicine extractive for resisting lung cancer A549 cells to proliferate, which is composed of the following materials by certain weight ratio: ternate buttercup root, cymose buckwheat rhizome, herba houttuyniae, agrimony, Chinese waxgourd seed, scarlet begonia, brazilwood, safflower, prepared pinellia tuber, arisaema cum bile, root of straight ladybell, polygonatum; the preparing method is that: the above 13 materials are added with water to be decocted and filtered so as to take the filtered liquid out; and then the filtered liquid is condensed to obtain a full extracting liquid 1; and then the extracting liquid 1 is extracted by sherwood oil so as to obtain a prepared extracting liquid 2; and finally, the prepared extracting liquid 2 can be upgraded by macroporous resin, eluted by pure water, and then eluted by ethanol; the eluted liquid is collected for condensing to 10g medicine/ml so as to obtain the Chinese traditional medicine extractive; indicated by the experiment result of pharmacodynamics, the Chinese traditional medicine extractive has obvious effects on resisting the lung cancer A549 cell proliferation.