Method for culturing lung cancer stem cells under 3D culture conditions
A technology of lung cancer stem cells and culture methods, applied in the field of conditional 3D cell culture, can solve the problems of small number of tumor stem cells, difficulty in separation and expansion, difficulty in establishing in vitro pharmacological models of tumor stem cells, etc.
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[0016] 1. Materials
[0017] Human lung adenocarcinoma cell line A549: purchased from the Cell Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, and preserved by our laboratory;
[0018] 10% fetal bovine serum: purchased from Hyclone Company of the United States;
[0019] RPMI-1640 complete medium: purchased from Hyclone Company of the United States;
[0020] Recovery solution: Cell Recovery Solution, 354253, purchased from BD Company in the United States;
[0021] BME (Eagle basal medium): purchased from BD Company in the United States;
[0022] PBS: Phosphate buffered saline (1x), 0.0067M (PO 4 );
[0023] Neutral metalloproteolytic enzyme: Dispase, 354235, purchased from BD Company of the United States;
[0024] Insulin-like growth factor-1 (IGF-1), purchased from Proteck;
[0025] Fibroblast growth factor (FGF) was purchased from Proteck.
[0026] 2. Method
[0027] (1) Cell culture
[0028] Six cell groups were set up, namely hig...
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