The invention discloses a method for measuring multiple parameters of
retina of a living mouse. The method comprises the following steps: fixing a mouse, which is successfully anesthetized, on a three-dimensional experiment table; assembling a plano-concave lens, a front lens and an
optical coherence tomography (OCT) instrument; adjusting the three-dimensional experiment table to ensure that the eyeballs of the mouse are positioned right ahead the front lens, and that a clear
retina scanning eye
fundus image appears on a display screen of the OCT instrument; selecting a high-definition one-
line scanning mode to obtain a cross-sectional image of the total
retina of the mouse, and measuring the thickness of each layer on the retina of the mouse; selecting a
retinal volume mode, aligning the center of a cross scanning line with the
optic disk of the mouse to measure the average thickness and volume of nine subareas of the retina of the mouse, and reconstructing the form of the retina of the mouse in a three-dimensional mode; and finally, selecting an
optic disk scanning mode to acquire continuous thickness data of a
nerve fiber layer of the retina. The method has the characteristics of high simplicity and feasibility, high
repeatability, accurate result and zero external environment interference and is suitable for popularization and application.