Multitudinous transplant method for woody flower group cultivated seedling

Abstract

The invention relates to a planting method for culturing test-tube seedlings by plant tissues, in particular to the mass transplanting technique for tissue cultured seedlings of woody flowers. The invention adopts a container which is different from conventional culture flasks for one-time transplanting. The structure of the container used for transplanting is like a cup without a handle, and the bottom of the cup is provided with at least one hole, the mouth of the cup is provided with a cover, and the cover and the upper part of the container are transparent. The transplanting method using the container comprises the following steps that: substrates are prepared, the substrates are sterilized and filled in the container; the tissue cultured seedlings are sterilized; the tissue cultured seedlings are transplanted; the substrates in which the tissue cultured seedlings are planted are drenched by thimerosal; and the mouth of the container is sealed by the cover. Due to the adoption of the method, the seedling raising time is shortened, the transplanting survival rate can be kept at more than 90 percent, and the manufacturing cost is saved by 30 percent; moreover, the method is also wide in application.

Description

technical field [0001] The invention relates to a cultivation method of plant tissue culture test-tube seedlings, in particular to a mass transplanting technique of woody flower tissue culture seedlings. Background technique [0002] As a means of rapid propagation, plant tissue culture has been widely used in the production of seedlings of forest trees, flowers and other crops. Whether the tissue-cultured seedlings propagated in vitro can be applied in large quantities and obtain good benefits depends on the transplanting of the tissue-cultured test-tube seedlings. The survival rate and survival quality of transplanting directly affect the production cost of seedlings. [0003] In the prior art, culture bottles are often used in the first generation, subgeneration, and rooting culture of plant tissue culture. When the tissue culture seedlings are cultivated in the bottle, they are always under high humidity, constant temperature, and weak light environment, resulting in the...

AI Technical Summary

Problems solved by technology

There are many procedures and complicated operations, which make it easy to reduce the survival rate of tissue cultured seedlings due to water loss during transplantation, and also take up a lot of labor, resulting in high production costs and low profits for tissue cultured seedlings.

Because of the difficulty in transplanting woody flower tissue cultured seedlings, people have done more research on the transplanting technology of tissue cultured seedlings, and have made some progress, but their research often stays in the laboratory research stage, or is limited to small batches. The scale of production does not meet the needs of large-scale production. The survival rate of large-scale transplanting is only 60% to 75%. Few people actually realize the industrialized production of woody flower tissue culture seedlings.

So far, there have been no reports on high-efficiency tissue-cultured seedling transplanting techniques applicable to many woody flower species with a survival rate of over 90% and low cost for mass transplanting of woody flower tissue-cultured seedlings