Composite nanodiamond medicine and preparation method and application thereof

A nano-diamond and drug technology, which is applied in drug combinations, pharmaceutical formulas, anti-tumor drugs, etc., can solve the problems of tumors that are difficult to eliminate and easy to relapse, and achieve excellent anti-cancer effects, good biocompatibility, and large specific surface area. Effect

Active Publication Date: 2018-10-16
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Even though the nano-diamond drug-carrying system is better than the free drug in many aspects, the tumor is still difficult to e

Method used

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  • Composite nanodiamond medicine and preparation method and application thereof
  • Composite nanodiamond medicine and preparation method and application thereof
  • Composite nanodiamond medicine and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A preparation method of nanodiamond / mitoxantrone / doxorubicin (ND / MTO / DOX) composite nanodiamond medicine, comprising the steps:

[0031] Weigh 1 mg ND, ultrasonically disperse it in sodium citrate (Na3Cit, 1.0 M, 1 mL) solution, add 240 μg MTO after 30 min, stir at room temperature for 1 h, centrifuge, wash with distilled water until the supernatant is colorless, collect Clear liquid, record liquid volume, get ND / MTO nano-medicine. Then ultrasonically disperse the ND / MTO nanomedicine in the sodium citrate solution. After 30 min, add 120 μg DOX, stir at room temperature for 1 h, centrifuge, wash with distilled water until the supernatant is colorless, collect the supernatant, and record the liquid volume. The obtained nano-diamond / mitoxantrone / doxorubicin (ND / MTO / DOX) composite nano-diamond drug is placed in a vacuum freeze dryer for drying, and is set aside.

[0032] By measuring the absorbance of DOX at 480nm (DOX ε480nm =11500cm -1 mol -1 ·L -1 ), the absorbance ...

Embodiment 2

[0034] Infrared spectrogram characterization

[0035] Through infrared spectroscopy, we can characterize the surface chemical properties of ND / MTO / DOX composite nanomedicine, determine whether MTO and DOX are adsorbed on the surface of ND, and whether the ND / MTO / DOX composite nanomedicine contains Na 3 Cit. Take a small amount of dried nanoparticles and KBr to mix and press into tablets, use an infrared spectrometer to measure their respective infrared spectra, and analyze the spectra.

[0036] figure 1 Infrared spectra of various materials. like figure 1 As shown in (A), in the infrared spectrum of ND, it can be seen that its surface is rich in carboxyl groups, indicating that the ND was successfully oxidized by strong acid; in the infrared spectrum of ND / MTO / DOX composite nanomedicine, the features marked by the frame Absorption peak and figure 1 (B)Na 3 The characteristic absorption peaks of the Cit infrared spectrum (marked in the frame) are consistent, indicating th...

Embodiment 3

[0038] To observe the morphology of various nanomaterials, we used scanning electron microscopy (SEM) to characterize ND, ND / MTO and ND / MTO / DOX nanomedicines. Take a small amount of dried nanoparticles on the conductive adhesive, paste the sample, spray gold, and test on the machine.

[0039] figure 2 It is the scanning electron microscope (SEM) image of ND, ND / MTO and ND / MTO / DOX nanomedicine. It can be seen from the figure that compared with ND, ND / MTO and ND / MTO / DOX have obvious stacked " Cloud clusters" (pointed by arrows) appear, and the "cloud clusters" on the ND / MTO / DOX surface are more obvious. This may be because the presence of sodium citrate induces the aggregation of MTO and DOX, and there is a π-π stacking phenomenon between ND, MTO and DOX. The experiment once again proved that the nanomedicine was successfully synthesized.

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Abstract

The invention provides a composite nanodiamond medicine and a preparation method and application thereof. Nanodiamond (ND) is adopted as a carrier by the medicine, and under the existence of sodium citrate, mitoxantrone (MTO) and doxorubicin (DOX) are sequentially loaded on the surfaces of the NDs by the way of physical adsorption, so that the composite nanomedicine (ND/MTO/DOX) is obtained. The change of cell mitochondrial membrane potential is detected by the JC-1 method, discovering that the degree of mitochondrial membrane potential decreases caused by the composite nanomedicine is greatest; the MTT method proves that the synergistic effect of MTO and DOX increases the ability of killing cancer cells to a great extent, and moreover, the composite nanomedicine has the characteristic ofslow-release medicine; a confocal result indicates that the ND/MTO/DOX composite nanomedicine is mainly located in lysosomes, moreover, as time goes on, the medicine is not migrated to cell nuclei, the system can probably cause an increase in lysosome permeability, so that a great deal of hydrolase can be released, and as a result, apoptosis is promoted. The composite nanodiamond medicine can be applied in the preparation of antitumor medicines.

Description

technical field [0001] The present invention relates to nano-medicine, in particular to a nano-diamond multi-drug loaded with two anticancer drugs at the same time, in particular to a nano-diamond / mitoxantrone / doxorubicin composite nano-diamond drug and its preparation method and application. Background technique [0002] The food safety and environmental pollution problems in today's society are becoming more and more serious, and the incidence of cancer is increasing year by year, which has become one of the most important human "killers". Currently, cancer treatment methods mainly include three conventional methods: surgery, radiation and chemical drug therapy. Among them, chemotherapy is widely used in clinic because of its simple operation, such as doxorubicin, methotrexate and mitoxantrone. Chemotherapy is effective to some extent, but its nonspecific biodistribution in normal tissues and its effects on healthy rapidly dividing cells lead to severe toxic side effects ...

Claims

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Application Information

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IPC IPC(8): A61K31/136A61K31/704A61K9/51A61K47/04A61P35/00
CPCA61K9/5115A61K31/136A61K31/704A61P35/00A61K2300/00
Inventor 李英奇杜祥斌赵文静
Owner SHANXI UNIV
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