The invention relates to a preparation method for a hydrophilic magnetic nano material for the enrichment of glycopeptides, belongs to the fields of nano materials and bio-separation, and aims at achieving the selective enrichment of the glycopeptides. The method is characterized in that a one-pot method is used for synthesizing carbohydrates, such as chitosan, cellulose and agarose, and the hydrophilic magnetic nano material compatible with ferroferric oxide, and due to the existence of -NH2 and -OH polar groups in carbohydrate molecules in the material, the material is has the certain hydrophilicity, and the glycopeptides can be specifically enriched and purified in complex hydrolyzed proteins. According to the method, good adsorption effects of the prepared enrichment material are ensured; in addition, the preparation method has the advantages of time saving, low cost, convenience in operation and the like, and is particularly applied to the large-scale enrichment of the glycopeptides, and a large amount of material can be prepared.