Preparation method for hydrophilic magnetic nano material for enrichment of glycopeptides

A magnetic nanometer and glycosylation technology, applied in the field of separation and purification of glycosylated peptides, can solve the problems of complicated operation and time-consuming

Inactive Publication Date: 2014-08-27
XINJIANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the synthesis of commonly used hydrophilic magnetic nanomaterials requires the synthesis of Fe 3 o 4 Nanoparticles coated with SiO 2 , then the SiO 2 The multi-step reaction such as grafting hydrophilic molecules after functionalization on the surface is cumbersome and time-consuming (Xiong Z. C. et al., Synthesis of branched PEG brushes hybrid hydraulic magnetic nanoparticles for the selective enrichment of N-linked glycopeptides, Chemical Communications, 48 ​​(2012 ), P8138-8140)

Method used

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  • Preparation method for hydrophilic magnetic nano material for enrichment of glycopeptides
  • Preparation method for hydrophilic magnetic nano material for enrichment of glycopeptides
  • Preparation method for hydrophilic magnetic nano material for enrichment of glycopeptides

Examples

Experimental program
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Effect test

Embodiment 1

[0015] 3 g FeCl 3 ·6H 2 O was added to 70 mL of ethylene glycol, ultrasonically dissolved for 20 min to form a yellow transparent solution, and 5 g of NH 4 Ac and 1.5 g chitosan, under nitrogen protection at 150 o C heated and stirred for 2.4 h, then transferred to a 100 mL reactor for 190 o C hydrothermal reaction for 18 h. After the reaction was completed, the cooled black solution was magnetically separated, and the product was washed with high-purity water and absolute ethanol respectively, 65 o C vacuum dried. The TEM and hysteresis loops of the material are shown in figure 1 : Its size is about 140 nm, and the material maintains good magnetic properties.

Embodiment 2

[0017] 1 g FeCl 3 ·6H 2 O was added to 25 mL of ethylene glycol, ultrasonically dissolved for 10 min to form a yellow transparent solution, and 1 g of NH 4 Ac and 0.5 g cellulose, under nitrogen protection at 180 o C heated and stirred for 4 h, then transferred to a 100 mL reactor at 250 o C hydrothermal reaction for 14 h. After the reaction was completed, the cooled black solution was magnetically separated, and the product was washed with high-purity water and absolute ethanol, respectively, for 50 o C vacuum dried.

Embodiment 3

[0019] 5 g FeCl 3 ·6H 2 O was added to 100 mL of ethylene glycol, ultrasonically dissolved for 30 min to form a yellow transparent solution, and 10 g of NH 4 Ac and 4 g agarose, under nitrogen protection at 130 o C heated and stirred for 1 h, then transferred to a 100 mL reactor for 150 o C hydrothermal reaction for 24 h. After the reaction was completed, the cooled black solution was magnetically separated, and the product was washed with high-purity water and absolute ethanol, respectively, for 80 o C vacuum dried.

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Abstract

The invention relates to a preparation method for a hydrophilic magnetic nano material for the enrichment of glycopeptides, belongs to the fields of nano materials and bio-separation, and aims at achieving the selective enrichment of the glycopeptides. The method is characterized in that a one-pot method is used for synthesizing carbohydrates, such as chitosan, cellulose and agarose, and the hydrophilic magnetic nano material compatible with ferroferric oxide, and due to the existence of -NH2 and -OH polar groups in carbohydrate molecules in the material, the material is has the certain hydrophilicity, and the glycopeptides can be specifically enriched and purified in complex hydrolyzed proteins. According to the method, good adsorption effects of the prepared enrichment material are ensured; in addition, the preparation method has the advantages of time saving, low cost, convenience in operation and the like, and is particularly applied to the large-scale enrichment of the glycopeptides, and a large amount of material can be prepared.

Description

technical field [0001] The invention relates to the separation and purification of glycosylated peptides, in particular to a method for preparing a hydrophilic magnetic nanometer material for enriching glycosylated peptides. Background technique [0002] The identification of protein post-translational modification sites is one of the important challenges in current proteomics research, and glycosylation modification is one of the most important post-translational modifications of proteins. Glycosylated proteins are involved in many important life processes such as cell signal transduction, cell adhesion, molecular clearance, and receptor activation. Abnormal glycosylation is closely related to the occurrence and development of many diseases. Many disease markers discovered so far are glycosylated proteins. The study of glycosylated proteins is of great significance for the diagnosis and treatment of biomedicine and diseases. Recently, mass spectrometry has been developed a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30B82Y40/00C07K1/22
Inventor 封顺方春丽徐世美王吉德
Owner XINJIANG UNIVERSITY
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