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Method for preparing total triterpenoids with hypoglycemic effect from leaves of Cyclocarya paliurus and application

A technology for total triterpenes and hypoglycemia, applied in the field of medicine and food, can solve problems such as no research or report on the preparation process of total triterpenes, and achieve the effects of simple and easy process steps, strong practicability, and increased glucose intake

Active Publication Date: 2019-02-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research or report about the preparation process for the preparation of total triterpenes with the polyamide material normal phase elution system as the core disclosed in this application

Method used

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  • Method for preparing total triterpenoids with hypoglycemic effect from leaves of Cyclocarya paliurus and application
  • Method for preparing total triterpenoids with hypoglycemic effect from leaves of Cyclocarya paliurus and application
  • Method for preparing total triterpenoids with hypoglycemic effect from leaves of Cyclocarya paliurus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dry 5 kg of Cyclocarya paliurus leaves, grind into powder, and extract twice at room temperature with 50 L of 80% ethanol solution, each time for 5 days. The combined extracts were concentrated under reduced pressure to dryness to obtain a total extract of 750 g. The total extract was dispersed with 1 L of water, and extracted with petroleum ether and chloroform in equal volumes respectively to obtain 280 g of the chloroform extract. The chloroform extraction part was eluted by a 60-80 mesh polyamide chromatographic column (dichloromethane: methanol = 15:1), and the eluted part was collected to obtain an extract. Then dissolve the extract with water, adsorb on the AB-8 macroporous resin column, first wash away impurities with 30% ethanol, and then elute with 60% ethanol. The 60% ethanol eluate was collected and concentrated to dryness under reduced pressure to obtain 105 g of dry powder of total triterpenes, with a total extraction rate of 2.1%. The total triterpene c...

Embodiment 2

[0022] 10 kg of dried Cyclocarya paliurus leaves were powdered and refluxed with 80 L of 70% ethanol solution twice for 2 hours each time. The combined extracts were concentrated under reduced pressure to dryness to obtain 1600 g of the total extract. The total extract was dispersed with 2L of water, extracted successively with equal volumes of petroleum ether and ethyl acetate to obtain 560 g of ethyl acetate extract. The ethyl acetate extraction part was eluted by a 100-200 mesh polyamide column (petroleum ether: acetone = 1:1), and the eluted part was collected to obtain an extract. Then dissolve the extract with water, adsorb on D101 macroporous resin column, first wash away impurities with 40% ethanol, and then elute with 70% ethanol. The 70% ethanol eluate was collected and concentrated to dryness under reduced pressure to obtain 280 g of dry powder of total triterpenes, with a total extraction rate of 2.8%. The measured triterpene content was 85.4%.

Embodiment 3

[0024] Dry 1 kg of Cyclocarya paliurus leaves, powder them, and extract twice with 10 L of 90% ethanol under reflux, each time for 100 min. The combined extracts were concentrated under reduced pressure to dryness to obtain a total extract of 182 g. The total extract was dispersed with 500 mL of water, extracted successively with equal volumes of petroleum ether and ethyl acetate: n-butanol (10:1), to obtain 65 g of ethyl acetate-n-butanol extract. The extracted part was eluted by a 100-200 mesh polyamide column (dichloromethane: ethyl acetate = 2:1), and the eluted part was collected to obtain the total triterpene extract. Then dissolve the extract with water, adsorb on the DM130 macroporous resin column, first wash away impurities with 50% ethanol, and then elute with 90% ethanol. The 90% ethanol eluate was collected and concentrated to dryness under reduced pressure to obtain 25 g of dry powder of total triterpenes with a total extraction rate of 2.5%. The measured triter...

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Abstract

The invention provides a method for preparing total triterpenoids with a hypoglycemic effect from leaves of Cyclocarya paliurus. The leaves of the Cyclocarya paliurus are taken as a raw material and subjected to extraction with an ethanol-water mixed solvent and liquid-liquid extraction with an organic solvent, polyamide column chromatography based organic solvent elution and macroporous resin column chromatography based ethanol-water elution are performed for separation and purification, and high-purity total triterpenoids can be obtained. Research proves that main components such as arjunolic acid, asiatic acid, cyclocaric acid B and a compound 4 in the total triterpenoids can effectively increase glucose uptake of 3T3-L1 adipose cells and have antidiabetic activity, and the total triterpenoids and the main components of the total triterpenoids can be used for preparing products such as hypoglycemic and antidiabetic drugs, health food and functional food. The method for preparing thetotal triterpenoids from the leaves of Cyclocarya paliurus and an application of the total triterpenoids and the compound 4 to preparation of the antidiabetic drugs are developed, reference is provided for popularization, utilization and deep processing of traditional Chinese medicinal materials, and a new application direction is provided.

Description

technical field [0001] The invention belongs to the technical field of medicine and food, and in particular relates to a method for preparing high-purity total triterpenes with hypoglycemic effect from Cyclocarya paliurus leaves, and the use of total triterpenes in the preparation of antidiabetic drugs, health food and functional food and other products in the application. Background technique [0002] Cyclocarya paliurus leaves are dried leaves of Cyclocarya paliurus (Batalin) Iljinsk. Cyclocarya paliurus is a unique single-species plant in my country, widely distributed in Jiangxi, Zhejiang, Anhui, Hunan, Guangdong and other areas south of the Yangtze River. Folks use its leaves as a tea drink. The tea tastes sweet and has the effects of promoting body fluids and quenching thirst, clearing heat and relieving summer heat, lowering blood sugar, lowering blood pressure and prolonging life. At the end of 2013, green willow leaves were approved by the National Health and Fami...

Claims

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Application Information

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IPC IPC(8): A61K36/52A61P3/10A23L33/105A61K127/00
CPCA23V2002/00A61K36/52A61K2236/333A61K2236/39A61K2236/51A61K2236/53A23L33/105A61P3/10A23V2200/328A23V2250/21
Inventor 甘礼社周长新方祝君莫建霞何钢锋
Owner ZHEJIANG UNIV
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