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Method for preparing asiatic centella total aglycone by asiatic centella total glycosides basic hydrolysis

A technology of centella asiaticosides and centella asiatica, which is applied in the field of preparing centella asiatica total aglycones by alkali hydrolysis of centella asiatica, can solve the problems of consuming a large amount of solvents, time-consuming and laborious, etc., and achieve the goal of inducing tumor cell apoptosis, Cardiovascular and cerebrovascular protection, easy to realize the effect of industrialization

Inactive Publication Date: 2009-06-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, the preparation method of total aglycones of Centella asiatica is mainly obtained by direct extraction from Centella asiatica grass, supplemented by purification processes such as extraction and chromatography, which not only consumes a lot of solvents, but also is time-consuming and laborious.

Method used

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  • Method for preparing asiatic centella total aglycone by asiatic centella total glycosides basic hydrolysis
  • Method for preparing asiatic centella total aglycone by asiatic centella total glycosides basic hydrolysis
  • Method for preparing asiatic centella total aglycone by asiatic centella total glycosides basic hydrolysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Add 500mL of 0.1mol / L NaOH aqueous solution into a 1L three-neck flask with a stirring and water condenser, put it in a constant temperature water bath at 90°C, start stirring and condensing water, and add 10g of the raw material of asiaticoside in 2 batches (after 2 hours of reaction Add the second batch) and hydrolyze for 8 hours; add hydrochloric acid to the hydrolyzate, adjust the pH value to 2-4, filter after precipitation to obtain a precipitate, and weigh the precipitate to obtain 4.2g after drying; collect the dry precipitate and add 21mL Dissolve in methanol, decolorize with activated carbon, filter, add 168mL of water to the filtrate to crystallize, obtain crystals after filtration, and then vacuum dry the crystals to obtain 2.5g total aglycone products of Centella asiatica, the product is analyzed by HPLC The mass content is 79.5%.

Embodiment 2

[0029] Add 500mL of 0.3mol / L KOH aqueous solution into a 1L three-necked flask with a stirring and water condenser, put it in a constant temperature water bath at 85°C, start stirring and condensing water, and add 20g of the raw material of Centella glycosides in 3 batches (total addition time 1h), hydrolyzed for 4h; add sulfuric acid to the hydrolyzate, adjust the pH value to 2-4, filter after precipitation to obtain a precipitate, and weigh the precipitate to obtain 8.1g after drying; collect the dried precipitate and add 81mL of ethanol to dissolve , activated carbon decolorization, filtration, the filtrate was added 810mL of water crystallization, the crystals were obtained after filtration, and the crystals were vacuum dried to obtain 5.8g total aglycone products of Centella asiatica, the product was analyzed by HPLC for the mass content of total aglycones of centella asiatica 89.6%.

Embodiment 3

[0031] Add 0.5mol / L Na to a 1L three-neck flask with stirring and water condenser 2 CO 3Put 500mL of aqueous solution in a constant temperature water bath at 80°C, turn on stirring and condense water, add 30g of asiaticoside raw material in 4 batches (the total feeding time is 1.5h), and hydrolyze for 5h; add hydrochloric acid to the hydrolyzate, and adjust the pH value to 2~4, filter after precipitation to get precipitate, weigh the precipitate to 12.3g after drying; add 184.5mL ethanol to dissolve the collected dry precipitate, decolorize with activated carbon, filter, add 2214mL water crystal to the filtrate, after filtration Crystals were obtained, and the crystals were vacuum dried to obtain 8.8 g of Centella asiatica aglycone product, the mass content of which was 90.1% by HPLC analysis.

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Abstract

The invention discloses a method for preparing asiaticoside by alkali hydrolysis of centella selected triterpenes, which comprises the following steps: 1) adding centella selected triterpenes raw material into an aqueous solution of inorganic base in batch for hydrolysis at a temperature of between 45 and 90 DEG C for 1 to 24 hours; 2) adding acid into the hydrolysate solution to adjust the pH value to be 2-4, depositing and filtering the hydrolysate solution to obtain deposit; and 3) drying the deposit, adding the deposit into an organic solvent for dissolution, decoloring the solution by active carbon, filtering the solution, adding water into filtrate for crystallization, filtering the filtrate to obtain crystals, and drying the crystals in vacuum to obtain the asiaticoside product. The asiaticoside product has high yield and purity, and can be used as bulk drug or further separated into different asiatic acid products; the asiaticoside product taken as a medicine can be used to treat skin wound, resist depressive illness and Alzheimer's disease, protect liver and veins of heart and brain, induce tumour cell apoptosis and so on; meanwhile, the method has simple technological process, small investment, and easy industrialization.

Description

technical field [0001] The invention relates to a method for preparing total aglycones of centella asiatica by hydrolyzing total glycosides of centella asiatica. Background technique [0002] Centella asiatica (L.) Urban is a plant of the genus Centella asiatica in the Umbelliferae family. It has a long history of application in the field of traditional medicine in many countries and regions. The internal and external use of Centella asiatica in traditional Chinese medicine has been It has a history of two thousand years. Centella asiatica is bitter in the mouth, pungent, cold in nature, returns liver, spleen, kidney, stomach meridian. According to ancient pharmacopoeia records, it is mainly used to treat diseases such as damp-heat jaundice, heatstroke diarrhea, sand and blood, carbuncle sores, and bruises. Modern pharmacological research and application have shown that Centella asiatica can be used to treat depression, skin wounds, gastric ulcers, infectious hepatitis, sk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 吕秀阳郑兴芳李音
Owner ZHEJIANG UNIV
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