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Method for extracting asiatic acid from centella

A technology of asiatic acid and centella asiatica, applied in the field of biological extraction, can solve the problems of large loss of target components, cumbersome, low purity of asiatic acid, etc.

Active Publication Date: 2017-01-25
桂林泰氪生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method is not only cumbersome, the organic solvent is not easy to separate, the loss of the target component is large, the yield is low, and the purity of the extracted Asiatic acid is also small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Crush Centella asiatica, add 60% ethanol solution 10 times the total weight of Centella asiatica, and reflux extract once, each time for 1 hour, filter the extract, recover the solvent from the filtrate, add 40°C water to dissolve, filter, and filter the residue Dissolve in 50v% ethanol solution to obtain crude extract;

[0021] 2) Adding 0.1% by weight of lanthanum chloride to the crude extract and mixing, stirring evenly to obtain a mixed solution;

[0022] 3) Put the mixed solution on the X-5 type macroporous resin column, the column speed is 0.5BV / h, first wash with water until colorless, and then elute with 20v% ethanol at a volume concentration of 2BV / h, thin Layer chromatography tracking detection, collecting ethanol eluate containing target components, recovering ethanol, drying to obtain asiatic acid.

[0023] Through HLPC analysis, the recovery rate of asiatic acid is above 95.16%, and the purity is as high as above 99.50%.

Embodiment 2

[0025] 1) Pulverize Centella asiatica, add 70% ethanol solution 20 times the total weight of Centella asiatica, and reflux extract 3 times, each time for 3 hours, combine the extracts, filter, recover the solvent from the filtrate, add 50 ° C water to dissolve, filter, Dissolving the filter residue in 60v% ethanol solution to obtain a crude extract;

[0026] 2) Add 0.5% of its weight of cerium sulfate to the crude extract and mix, stir well to obtain the mixed solution;

[0027] 3) Put the mixed solution on the X-5 type macroporous resin column, the column loading speed is 0.8BV / h, first wash with water until colorless, then elute with volume concentration of 30v% ethanol, the elution speed is 2.5BV / h, Thin-layer chromatography tracking detection, collecting ethanol eluate containing target components, recovering ethanol, drying to obtain asiatic acid.

[0028] Through HLPC analysis, the recovery rate of asiatic acid is above 95.08%, and the purity is as high as above 99.58%....

Embodiment 3

[0030] 1) Pulverize Centella asiatica, add 65% ethanol solution 15 times the total weight of Centella asiatica, and reflux extract twice, each time for 2 hours, combine the extracts, filter, recover the solvent from the filtrate, add 45 ° C water to dissolve, filter, Dissolving the filter residue in 55v% ethanol solution to obtain a crude extract;

[0031] 2) Adding 0.3% by weight of praseodymium nitrate to the crude extract and mixing, stirring evenly to obtain a mixed solution;

[0032] 3) Put the mixed solution on the X-5 type macroporous resin column, the column loading speed is 0.6BV / h, wash with water until colorless, and then elute with ethanol with a volume concentration of 25v%, the elution speed is 2.2BV / h, Thin-layer chromatography tracking detection, collecting ethanol eluate containing target components, recovering ethanol, drying to obtain asiatic acid.

[0033] Through HLPC analysis, the recovery rate of asiatic acid is above 95.42%, and the purity is as high a...

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Abstract

The invention discloses a method for extracting asiatic acid from centella. The method comprises the following steps: 1) pulverizing centella, extracting with a 60-70 v% ethanol solution under reflux, filtering the extracting solution, recovering the solvent from the filtrate, adding 40-50-DEG C for dissolution, filtering, and dissolving the filter residue in a 50-60 v% ethanol solution to obtain a crude extract; 2) adding a rare-earth salt into the crude extract, and mixing and stirring uniformly to obtain a mixed solution; and 3) passing the mixed solution through an X-5 macroporous resin column, washing with water to a colorless state, eluting with 20-30 v% ethanol, carrying out thin-layer chromatography tracking detection, collecting the ethanol eluate containing the target component, recovering the ethanol, and drying to obtain the asiatic acid. The asiatic acid extracted by the method has the advantages of high purity and high yield.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting asiatic acid from centella asiatica. Background technique [0002] Centella asiatica (L.) Urban is a Centella asiatica tree plant in the Umbelliferae family. Because its leaves resemble a horseshoe or half a copper coin, it is also known as horseshoe grass, even money grass, etc., and some people call it Peppermint, Dimograss, etc. Centella asiatica has a long history of application in the field of traditional medicine in many countries and regions. The internal and external use of Centella asiatica in traditional Chinese medicine in my country has a history of two thousand years, and it was first recorded in the book "Shen Nong's Herbal Classic". According to the "Compendium of Materia Medica", Centella asiatica tastes bitter, pungent, cold in nature, non-toxic, and returns to the liver, spleen, kidney, and stomach meridians. T...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 谢冬养黄健军陆美珍叶出良
Owner 桂林泰氪生物科技有限公司
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