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Process for extracting asiatic acid from herba centellae

A technology of asiatic acid and centella asiatica, applied in the production of steroids, bulk chemicals, organic chemistry, etc., can solve the problem that the sample cannot be used as a qualitative reference.

Inactive Publication Date: 2017-07-14
南宁馨艺荣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention provides a process for extracting Asiatic acid from Centella asiatica, in order to solve the problem that the purity of Asiatic acid prepared by the prior art is above 95%, and the obtained sample cannot be completely used as a qualitative reference substance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A process for extracting asiatic acid from centella asiatica, comprising the following steps:

[0041] S1: crushing Centella Asiatica and passing through a 50-mesh sieve to obtain Centella Asiatica powder;

[0042] S2: Add 10 times its weight of 66% methanol to the centella asiatica powder prepared in step S1, reflux extraction twice under water bath conditions, each extraction time is 1.8h, combine the extract after the extraction is complete, and adjust the extract The pH value was adjusted to 3.5, the temperature was controlled at 56°C, decolorized and impurity removed by activated carbon for 0.8h, and a decolorized solution was obtained after filtration;

[0043] S3: Concentrate and dry the primary decolorization solution obtained in step S2 to obtain an extract extract, which is dissolved in water at 46° C. with 5.2 times the volume of the extract extract, extracted twice with anhydrous ethyl acetate solvent, and continued to use Anhydrous ethyl acetate is carried...

Embodiment 2

[0049] A process for extracting asiatic acid from centella asiatica, comprising the following steps:

[0050] S1: crushing Centella Asiatica and passing through a 30-mesh sieve to obtain Centella Asiatica powder;

[0051] S2: Add 9 times of its weight 63% methanol to the Centella asiatica powder prepared in step S1, reflux and extract twice under water bath conditions, each extraction time is 2h, combine the extract after the extraction is complete, and adjust the concentration of the extract pH value to 3.2, temperature controlled at 50°C, decolorized and impurity removed by activated carbon for 1 hour, and filtered to obtain a primary decolorized solution;

[0052]S3: Concentrate and dry the primary decolorization solution obtained in step S2 to obtain an extract extract, which is dissolved in water at 40° C. with 5 times the volume of the extract extract, extracted twice with anhydrous ethyl acetate solvent, and continued to use Anhydrous ethyl acetate is carried out cryst...

Embodiment 3

[0058] A process for extracting asiatic acid from centella asiatica, comprising the following steps:

[0059] S1: crushing Centella Asiatica and passing through a 60-mesh sieve to obtain Centella Asiatica powder;

[0060] S2: Add 67% methanol 11 times its weight to the centella asiatica powder prepared in step S1, reflux extraction under water bath conditions for 3 times, each extraction time is 1.5h, combine the extract after the extraction is complete, and adjust the extract pH value to 3.6, temperature controlled at 60°C, decolorized and impurity removed by activated carbon for 0.5h, and filtered to obtain a decolorized solution;

[0061] S3: Concentrate and dry the primary decolorization solution obtained in step S2 to obtain an extract extract, which is dissolved in water at 50°C with 5.5 times the volume of the extract extract, and extracted 3 times with anhydrous ethyl acetate solvent, and then continue to use Anhydrous ethyl acetate was crystallized three times, and t...

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Abstract

The invention discloses a process for extracting asiatic acid from herba centellae and belongs to the technical field of preparation of the asiatic acid. The asiatic acid is prepared through the following steps of crushing, carrying out primary de-coloring, crystallizing, carrying out secondary de-coloring, recrystallizing, extracting, separating and purifying, concentrating and crystallizing, drying and the like. According to the method disclosed by the invention, the extraction yield of the asiatic acid is 64.8 percent or more and the purity of the asiatic acid is 98.09 percent or more, the purity is higher than that of the asiatic acid prepared in the prior art and the asiatic acid can be used as a qualitative and quantitative reference substance.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of asiatic acid preparation, in particular to a process for extracting asiatic acid from Centella asiatica. [0003] 【Background technique】 [0004] Asiatic acid, ursane-type pentacyclic triterpene acids. Asiatic acid is insoluble in petroleum ether and water, soluble in methanol, ethanol, butanol, butanone, slightly soluble in acetone, slightly soluble in benzene, chloroform, ether and other organic solvents. [0005] Centella asiatica is the dry whole herb of the Umbelliferae plant Centella asiatica, also known as Luodeda, Bengdawan, and Banbianqian. It has been used as a medicinal plant in my country for more than 2,000 years, and it is included in the "Chinese Pharmacopoeia" commonly used traditional Chinese medicine. Centella asiatica resources are very rich in China, almost all over the south of the Yangtze River Basin. Centella asiatica has the effects of clearing heat and dampness, detoxify...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008Y02P20/54
Inventor 黄健军谢冬养梁可文黄能梁可武
Owner 南宁馨艺荣生物科技有限公司
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