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Method for preparing asiatic acid by hydrolyzing asiaticoside

A technology of asiaticoside and asiatic acid, which is applied in the field of hydrolyzing asiaticoside to prepare asiatic acid, which can solve the problems of reducing the yield and purity of target components, and achieve the effect of complete hydrolysis and mild hydrolysis process

Active Publication Date: 2017-01-25
广西惠康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method adopts high temperature hydrolysis, which will cause dehydration, cyclization, double bond displacement, substituent displacement, configuration transformation and other changes of asiatic acid, which will greatly reduce the yield and purity of the target component

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) 1g asiaticoside, 3.44×10 -2 Mix 1 mol sodium lauryl sulfate and 0.01 mol NaOH, add water to 1L, stir and dissolve to obtain a mixed solution, hydrolyze at normal temperature and pressure for 10-15 minutes, and obtain a hydrolyzate;

[0019] 2) Use HCl to adjust the pH value of the hydrolyzate to 2, let stand, filter, and collect the precipitate;

[0020] 3) Dissolve the precipitate in ethanol with a volume concentration of 50% according to the solid-liquid ratio of 1g: 5ml, filter, add the filtrate to water 8 times the volume of ethanol for crystallization, filter to obtain crystals, and dry to obtain Asiatic acid The finished product weighs 0.434g, and its purity is 95.6% by HPLC analysis. The yield was 90.59%.

Embodiment 2

[0022] 1) 1g asiaticoside, 4.3×10 -2 Mix 1 mol sodium lauryl sulfate and 0.02 mol NaOH, add water to 1L, stir and dissolve to obtain a mixed solution, hydrolyze at normal temperature and pressure for 15 minutes, and obtain a hydrolyzed solution;

[0023] 2) Use HCl to adjust the pH value of the hydrolyzate to 5, let stand, filter, and collect the precipitate;

[0024] 3) Dissolve the precipitate in ethanol with a volume concentration of 70% according to the ratio of material to liquid: 1g: 30ml, filter, add the filtrate to water 16 times the volume of ethanol to crystallize, filter to obtain crystals, and dry to obtain asiatic acid The finished product weighs 0.445g, and its purity is 95.8% by HPLC analysis. The yield is 93.08%.

Embodiment 3

[0026] 1) 1g asiaticoside, 3.87×10 -2 Mix 1 mol sodium lauryl sulfate and 0.015 mol NaOH, add water to 1 L, stir and dissolve to obtain a mixed solution, hydrolyze at normal temperature and pressure for 12 minutes, and obtain a hydrolyzate;

[0027] 2) Use HCl to adjust the pH value of the hydrolyzate to 3, let it stand, filter, and collect the precipitate;

[0028] 3) Dissolve the precipitate in ethanol with a volume concentration of 60% according to the solid-liquid ratio of 1g: 15ml, filter, add the filtrate to water 12 times the volume of ethanol for crystallization, filter to obtain crystals, and dry to obtain Asiatic acid The finished product weighs 0.439g, and its purity is 95.0% by HPLC analysis. The yield is 91.06%.

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PUM

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Abstract

The invention discloses a method for preparing asiatic acid by hydrolyzing asiaticoside. The method comprises the following steps: 1) mixing asiaticoside, lauryl sodium sulfate and NaOH, adding water for dissolution, and hydrolyzing at normal temperature for 10-15 minutes; 2) regulating the pH value of the hydrolysate to 2-5, standing, filtering, and collecting the precipitate; and 3) dissolving the precipitate in ethanol, filtering, adding water to the filtrate, crystallizing, filtering to obtain the crystal, and drying to obtain the finished product. The method can quickly cut off glycosidic bonds under the conditions of normal temperature and pressure, has the advantages of mild hydrolytic process and thorough hydrolysis, and can not generate configurative changes of asiatic acid.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for preparing asiatic acid by hydrolyzing asiaticoside. Background technique [0002] Centella asiatica (L.) Urban is a Centella asiatica tree plant in the Umbelliferae family. Because its leaves resemble a horseshoe or half a copper coin, it is also known as horseshoe grass, even money grass, etc., and some people call it Peppermint, Dimograss, etc. Centella asiatica has a long history of application in the field of traditional medicine in many countries and regions. The internal and external use of Centella asiatica in traditional Chinese medicine in my country has a history of two thousand years, and it was first recorded in the book "Shen Nong's Herbal Classic". According to the "Compendium of Materia Medica", Centella asiatica tastes bitter, pungent, cold in nature, non-toxic, and returns to the liver, spleen, kidney, and stomach meridians...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 谢冬养黄健军陆美珍叶出良
Owner 广西惠康生物科技股份有限公司
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