Method for producing high-quality collagen oligopeptide by using fish skin or fishbone

Abstract

The invention provides a method for producing high-quality collagen oligopeptide by using fish skin or fishbone. The method comprises the following steps of: washing and crushing the fish skin or fishbone as a raw material, mixing the crushed material and water according to a weight ratio of 1:(5-20), carrying out refining treatment, then sterilizing and cooling, simultaneously adding proteolytic enzyme and lactobacillus to carry out primary hydrolysis, then centrifuging and filtering to separate a non-hydrolyzed solid part and hydrolysate, carrying out ultrafiltration on the hydrolysate by adopting an ultrafiltration film, mixing a macromolecular peptide solution which does not pass through the ultrafiltration film and the non-hydrolyzed solid part which is separated after the primary hydrolysis is finished, simultaneously adding protease and lactobacillus to carry out secondary hydrolysis, carrying out centrifugal separation and filtration, then carrying out ultrafiltration, and sequentially purifying, sterilizing, condensing and drying a micromolecular oligopeptide solution which passes through the ultrafiltration film and micromolecular oligopeptide collected after the primary hydrolysis to obtain the collagen oligopeptide product with low molecular weight. According to the method provided by the invention, the utilization rate and the yield of the raw material protein can be effectively improved, and the product quality can be greatly improved.

Description

technical field [0001] The invention relates to a method for preparing collagen peptides, in particular to a method for producing high-quality collagen oligopeptides by using fish skin or fish bones. Background technique [0002] Collagen is a high-molecular protein, which is the most abundant protein in animals, accounting for about 25%-33% of human protein, equivalent to 6% of human body weight. Collagen is a three-dimensional helical protein, mainly found in Human skin, bones, eyes, teeth, internal organs and other parts are important raw materials for repairing various damaged tissues. Collagen can keep the skin elastic and moist, keep the skin delicate and smooth, and has obvious effects in repairing the skin. Collagen peptide is a kind of peptide that is processed by proteolytic hydrolysis with collagen as raw material, and its molecular weight is much smaller than that of protein. The peptide containing more than two but less than ten amino acids in collagen peptide i...

AI Technical Summary

Problems solved by technology

However, most of the currently disclosed collagen peptide preparation methods only use one-time hydrolysis. During the hydrolysis process, there are defects such as low degree of hydrolysis and low protein utilization rate, resulting in a lot of waste of raw materials. The protein peptide after hydrolysis has a large molecular weight, and the molecular weight of the peptide has an important impact on the quality of the peptide product

[0005] Tianjin Business School applied for a patent number 200410096892.6 on December 10, 2004. The Chinese patent titled "A technology for efficient enzymatic hydrolysis of zein to prepare biologically active oligopeptides" mentioned that secondary enzymatic hydrolysis can be carried out, but the The method is aimed at the preparation of plant protein peptides. There is a big difference between extracting protein peptides from plants and extracting protein peptides from animals.

In addition, because aquatic animals have a strong fishy smell, especially some deep-sea fish, the fishy smell is even heavier, so if the collagen peptide extracted from fish is not handled properly, there will be some peculiar smell, and the product is inferior in flavor. good

At present, some methods for preparing collagen from aquatic animals are also disclosed. These methods involve the step of removing fishy smell. Generally, they are washed with salt water before hydrolysis, and after hydrolysis, yeast or lactic acid bacteria are used to ferment for several hours to remove fishy smell. Patent application number: 200810029603.9, Invention name: A method for preparing fish protein powder without bitter smell, Chinese patent application number: 200610019333.4, Invention name: Preparation method of freshwater fish protein active polypeptide hydrolyzate, Chinese patent application number: 201110121718.2 A method for removing the fishy smell of marine fish enzymatic hydrolyzate, etc. These methods increase the treatment process, or due to the limited processing time, the fishy smell cannot be completely removed. In addition, the utilization rate of raw protein cannot be improved, resulting in production costs. increase and waste of raw materials

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