10 results about "Insect Gene" patented technology

The present application belongs to the field of biotechnology, and particularly relates to a method for inducing expression of a target protein gene based on juvenile hormone and application thereof. The present application provides a method for inducing expression of a target protein gene based on juvenile hormone on the basis of long-term research on juvenile hormone regulating expression of an insect gene. The method induces protein expression by juvenile hormone, realizes controllable switch of protein expression, and reduces side effects of protein expression on organisms. The method is harmless to human and mammals, safe to other organisms and environment, and can be used for disease treatment, immunity enhancement, and new function of organisms. The method has good practical application value in disease treatment, disease resistance of animals and plants, and new function of organisms.

The invention provides a system and a method for complement expression after migratory locust gene knockout, and belongs to the technical field of insect gene editing, the system for complement expression after migratory locust gene knockout comprises an overexpression plasmid and a living transfection reagent; the overexpression plasmid carries a GAPDH (glyceraldehyde-phosphate dehydrogenase) promoter and a complement gene; the method comprises the following steps: 1) cloning a back-filling gene to a carrier skeleton carrying a GAPDH promoter; 2) respectively dissolving the overexpression plasmid and the living transfection reagent, and then mixing and incubating to obtain a system for complement expression after migratory locust gene knockout; 3, the system is delivered to a migratory locust living body, by means of the system for complement expression after migratory locust gene knockout, the expression quantity of the gene in a migratory locust gene knockout mutant can be specifically complemented, complement expression of the target gene can be detected at the mRNA and protein level, and an effective means is provided for accurately verifying the function of the knockout gene.

This invention provides an injection solution for single-base site-directed editing in locusts and a gene editing method for protein sequence modification using single-base site-directed editing in locusts, belonging to the field of insect gene editing technology. The injection solution comprises the following components at final concentrations: 380-420 ng / μL Cas9, 130-170 ng / μL sgRNA, and 280-320 ng / μL ssODN donor. The injection solution is microinjected into locust eggs, and single-base edited individuals are screened. The method of this invention can specifically alter transcription factors. CAMTA A single base in the protein-coding region of a gene can cause a desired change in the amino acid encoded by the corresponding triplet codon, thereby achieving changes at the protein level from single-base editing. This enables diversified regulation of protein amino acid sequences and functions, providing a new technical approach for locust gene function analysis and pest control research.

The invention relates to an insect-resistant gene OsTIR1 and application thereof. Specifically, the invention relates to application of an OsTIR1 gene and / or an OsTIR1 protein as a target spot in regulation and control of insect resistance of plants.

The invention belongs to the technical field of gene engineering and biological prevention and control, and particularly relates to a human single-domain antibody insect-resistant gene C4D, a protein human single-domain antibody C4D coded by the same and application of the human single-domain antibody insect-resistant gene C4D. The humanized single-domain antibody C4D disclosed by the invention can be specifically combined with the BBMV of the middle intestine of the plutella xylostella. The determination result of the indoor insecticidal biological activity of the plutella xylostella shows that the death rate within 5 days is 47.78 + / -4.16% under the concentration of the specific humanized single-domain antibody C4D. Meanwhile, the human single-domain antibody C4D is obtained by screening from a human single-domain antibody library, has the characteristics of small molecular weight and simple preparation, has lower heterology compared with antibodies from other sources, causes small immune response in a human body, is beneficial for improving safety as an insect-resistant material, and has a broad application prospect. The method has important scientific and practical significance for reducing various safety risks caused by wide use of the existing Bt toxin and expanding and enriching novel insecticidal gene resources.

The invention provides an injection for migratory locust single-base fixed-point editing and a migratory locust single-base fixed-point editing gene editing method for protein sequence modification, and belongs to the technical field of insect gene editing. The injection provided by the invention comprises the following components in final concentration: 380 to 420 ng / [mu] L of Cas9, 130 to 170 ng / [mu] L of sgRNA, and 280 to 320 ng / [mu] L of an ssODN donor. The injection is microinjected into locust eggs, and single-base edited individuals are obtained through screening. According to the method disclosed by the invention, a single basic group in a transcription factor CAMTA gene protein coding region can be specifically changed, so that amino acid coded by a corresponding triad codon is subjected to expected change, the change from single basic group editing to a protein level is realized, diversified regulation and control of protein amino acid sequences and functions are realized, and the application prospect is wide. And a new technical approach is provided for migratory locust gene function analysis and pest prevention and control research.

This invention relates to a method and system for predicting insect multi-tissue expression levels based on multimodal feature fusion. Using the target gene as the basic prediction unit, it simultaneously inputs the target gene's regulatory sequence information, RNA stability characteristics, and protein representation information. Joint modeling is performed through a multi-tower deep learning network, outputting predicted expression levels of the target gene in multiple tissues. Advantages: It overcomes the limitations of traditional methods that primarily rely on single-modality data. For the first time, it integrates insect gene regulatory sequences, RNA stability features, and pre-trained protein language models into a unified expression prediction framework. Utilizing the multimodal prediction model, it extracts, fuses, and predicts multimodal data features, providing accurate and comprehensive gene expression level prediction results. Furthermore, it introduces a gating backoff mechanism for missing protein annotations, avoiding the misinterpretation of missing values ​​as zero or noise, thus ensuring prediction stability.

The invention relates to an insect-resistant gene OsIAA10 and application thereof. Specifically, the invention relates to application of an OsIAA10 gene and / or an OsIAA10 protein as a target spot in regulation and control of insect resistance of plants.

This invention relates to the field of nanomaterials and gene interference, specifically disclosing a method for interfering with insect gene expression by infiltrating dsRNA through the insect's body wall. The method involves complexing a nanocarrier with a functional macromolecule of dsRNA, and with the aid of a surfactant, enabling exogenous dsRNA molecules to attach, spread, penetrate the body wall, and enter various organs and tissues within the insect, thus efficiently interfering with gene expression. This invention utilizes the dual synergistic effect of the nanocarrier and the surfactant to achieve efficient and non-invasive delivery of exogenous dsRNA molecules into insect cells, ultimately promoting the correct targeting of dsRNA molecules and improving RNA interference efficiency. The method described in this invention is simple, efficient, and has low error, and has significant practical implications for scientific research on insect gene interference and the application of highly efficient, safe, and targeted RNA pesticide formulations.