21 results about "Insect Gene" patented technology

The invention is suitable for the technical field of insect genetic engineering, and provides an HparGR22 gene and application thereof in establishing a phlorizin recognition in-vitro model. The DNA sequence of the HparGR22 gene is as shown in SEQ ID No: 1, and the coded amino acid sequence is as shown in SEQ ID No: 2. The gene has the highest expression quantity in the lower labial tassel of a holotrichia parallela larva, an oocyte injected with cRNA of the gene has obvious response to stimulation of phlorizin with different concentrations, it is clear that a product expressed by the HparGR22 gene can recognize phlorizin, and the function of the HparGR22 gene as the phlorizin receptor gene of the holotrichia parallela is determined. In view of the fact that the phlorizin recognition function of the gene is clear for the first time, a basis is provided for application of the gene in establishment of a phlorizin recognition in-vitro model, a foundation is laid for exploring the potential value of the gene in the field of gene engineering for preventing and controlling holotrichia parallela, and meanwhile a new direction is opened up for further research and application of the gene.

The invention relates to the field of insect gene analysis, and discloses an insect genomics data weighted sparse principal component analysis method based on adaptive noise cancellation regularization, comprising: acquiring a data set including an insect genome data set and a gene pathway data set; carrying out noise removal processing by adopting a self-adaptive noise elimination regularizer; constructing a weighted gene network in the edge group sparse principal component analysis model, and performing model training based on the data set to obtain a gene analysis model; and outputting gene expression values with different weights based on the gene analysis model. The adaptive noise elimination regularization method is adopted to solve the noise interference in the data, and the weighted gene network is utilized to enhance the biological interpretation capability of the model so as to improve the gene analysis accuracy.

The present invention relates to the technical field of genetic transformation of insect eggs. Specifically, the present invention refers to an efficient genetic editing system to obtain recombinant or genetically modified insect eggs, by incorporating genetic material directly into oocytes of female insects, which will then generate a large number of eggs with the incorporated or recombinant genetic material.

The present application belongs to the field of biotechnology, and particularly relates to a method for inducing expression of a target protein gene based on juvenile hormone and application thereof. The present application provides a method for inducing expression of a target protein gene based on juvenile hormone on the basis of long-term research on juvenile hormone regulating expression of an insect gene. The method induces protein expression by juvenile hormone, realizes controllable switch of protein expression, and reduces side effects of protein expression on organisms. The method is harmless to human and mammals, safe to other organisms and environment, and can be used for disease treatment, immunity enhancement, and new function of organisms. The method has good practical application value in disease treatment, disease resistance of animals and plants, and new function of organisms.

This invention relates to the field of insect genetic engineering technology and provides a dark-browed scarab beetle. HparGR16 Genes and their applications. Genes from the dark-breasted scarab beetle involved in CO2 recognition. HparGR16 The gene, whose DNA sequence is shown in SEQ ID No:1, and the amino acid sequence of the protein encoded by the gene is shown in SEQ ID No:2. This invention is the first to demonstrate... HparGR16 The gene is the CO2 receptor gene of the dark-browed scarab beetle. By interfering with the expression of this gene, or by designing, synthesizing, and screening compounds that can inhibit its protein activity, the CO2 recognition process of the dark-browed scarab beetle can be effectively blocked. This characteristic makes this gene a target for designing and screening control agents, thereby developing novel pest behavior regulators and insecticides, achieving efficient and green control of the dark-browed scarab beetle. This has important application value in the field of genetic engineering for the control of the underground pest, the dark-browed scarab beetle.

The invention provides a system and a method for complement expression after migratory locust gene knockout, and belongs to the technical field of insect gene editing, the system for complement expression after migratory locust gene knockout comprises an overexpression plasmid and a living transfection reagent; the overexpression plasmid carries a GAPDH (glyceraldehyde-phosphate dehydrogenase) promoter and a complement gene; the method comprises the following steps: 1) cloning a back-filling gene to a carrier skeleton carrying a GAPDH promoter; 2) respectively dissolving the overexpression plasmid and the living transfection reagent, and then mixing and incubating to obtain a system for complement expression after migratory locust gene knockout; 3, the system is delivered to a migratory locust living body, by means of the system for complement expression after migratory locust gene knockout, the expression quantity of the gene in a migratory locust gene knockout mutant can be specifically complemented, complement expression of the target gene can be detected at the mRNA and protein level, and an effective means is provided for accurately verifying the function of the knockout gene.

The present invention belongs to the technical field of insect genetic engineering, and particularly relates to the Or‑43884 gene of Coptotermes formosanus and its application in termite control. The Or‑43884 nucleotide sequence of the gene is shown in SEQ ID NO.1. The present invention discovers an odorant receptor protein gene of Coptotermes formosanus Or‑43884 gene. Through its expression in Xenopus laevis oocytes and molecular docking analysis of the Or-43884 protein, it is found that Or‑43884 the gene and its expression product are key odorant receptor genes for Coptotermes formosanus to recognize odor substances to trigger the nest cleaning behavior. Or‑43884 This function of the gene can be combined with the control methods of applying drugs and pathogenic microorganisms against termites, providing a new approach for termite control work.

This invention provides an injection solution for single-base site-directed editing in locusts and a gene editing method for protein sequence modification using single-base site-directed editing in locusts, belonging to the field of insect gene editing technology. The injection solution comprises the following components at final concentrations: 380-420 ng / μL Cas9, 130-170 ng / μL sgRNA, and 280-320 ng / μL ssODN donor. The injection solution is microinjected into locust eggs, and single-base edited individuals are screened. The method of this invention can specifically alter transcription factors. CAMTA A single base in the protein-coding region of a gene can cause a desired change in the amino acid encoded by the corresponding triplet codon, thereby achieving changes at the protein level from single-base editing. This enables diversified regulation of protein amino acid sequences and functions, providing a new technical approach for locust gene function analysis and pest control research.

The invention relates to an insect-resistant gene OsTIR1 and application thereof. Specifically, the invention relates to application of an OsTIR1 gene and / or an OsTIR1 protein as a target spot in regulation and control of insect resistance of plants.

The invention belongs to the technical field of gene engineering and biological prevention and control, and particularly relates to a human single-domain antibody insect-resistant gene C4D, a protein human single-domain antibody C4D coded by the same and application of the human single-domain antibody insect-resistant gene C4D. The humanized single-domain antibody C4D disclosed by the invention can be specifically combined with the BBMV of the middle intestine of the plutella xylostella. The determination result of the indoor insecticidal biological activity of the plutella xylostella shows that the death rate within 5 days is 47.78 + / -4.16% under the concentration of the specific humanized single-domain antibody C4D. Meanwhile, the human single-domain antibody C4D is obtained by screening from a human single-domain antibody library, has the characteristics of small molecular weight and simple preparation, has lower heterology compared with antibodies from other sources, causes small immune response in a human body, is beneficial for improving safety as an insect-resistant material, and has a broad application prospect. The method has important scientific and practical significance for reducing various safety risks caused by wide use of the existing Bt toxin and expanding and enriching novel insecticidal gene resources.

The invention provides an injection for migratory locust single-base fixed-point editing and a migratory locust single-base fixed-point editing gene editing method for protein sequence modification, and belongs to the technical field of insect gene editing. The injection provided by the invention comprises the following components in final concentration: 380 to 420 ng / [mu] L of Cas9, 130 to 170 ng / [mu] L of sgRNA, and 280 to 320 ng / [mu] L of an ssODN donor. The injection is microinjected into locust eggs, and single-base edited individuals are obtained through screening. According to the method disclosed by the invention, a single basic group in a transcription factor CAMTA gene protein coding region can be specifically changed, so that amino acid coded by a corresponding triad codon is subjected to expected change, the change from single basic group editing to a protein level is realized, diversified regulation and control of protein amino acid sequences and functions are realized, and the application prospect is wide. And a new technical approach is provided for migratory locust gene function analysis and pest prevention and control research.

This invention relates to a method and system for predicting insect multi-tissue expression levels based on multimodal feature fusion. Using the target gene as the basic prediction unit, it simultaneously inputs the target gene's regulatory sequence information, RNA stability characteristics, and protein representation information. Joint modeling is performed through a multi-tower deep learning network, outputting predicted expression levels of the target gene in multiple tissues. Advantages: It overcomes the limitations of traditional methods that primarily rely on single-modality data. For the first time, it integrates insect gene regulatory sequences, RNA stability features, and pre-trained protein language models into a unified expression prediction framework. Utilizing the multimodal prediction model, it extracts, fuses, and predicts multimodal data features, providing accurate and comprehensive gene expression level prediction results. Furthermore, it introduces a gating backoff mechanism for missing protein annotations, avoiding the misinterpretation of missing values ​​as zero or noise, thus ensuring prediction stability.

The invention belongs to the technical field of insect genes, and particularly relates to a brown planthopper transcription factor CEBP and application thereof. According to the invention, a promoter sequence at the upstream of a brown planthopper membrane-bound trehalase gene NlTRE2 gene segment is cloned from brown planthopper, a transcription factor CEBP is predicted and verified to promote the expression of a trehalase gene promoter, and RNAi interference is utilized to prove that CEBP enables the trehalase gene NlTRE2 gene segment to have low expression; therefore, the sensitivity of the brown planthopper to buprofezin medicines is improved. The invention provides a new method for enhancing the sensitivity of brown planthopper to buprofezin drugs, and lays a foundation for understanding the relationship between energy metabolism and drug resistance of brown planthopper and for high-efficiency and low-toxicity anti-brown planthopper drugs of brown planthopper.

The invention relates to an insect-resistant gene OsIAA10 and application thereof. Specifically, the invention relates to application of an OsIAA10 gene and / or an OsIAA10 protein as a target spot in regulation and control of insect resistance of plants.

The invention belongs to the technical field of insect genes, and particularly relates to a transcription factor of a brown planthopper trehalase gene and application of the transcription factor. According to the invention, a soluble trehalase NlTRE1-1 gene promoter sequence is cloned from brown planthopper, an active fragment is analyzed through dual luciferase, and an efficient transcription factor Adf-1 combined with the active fragment is obtained. Furthermore, the invention designs and synthesizes an RNAi fragment combined with Adf-1, and finds that silence of Adf-1 can influence the expression of NlTRE1-1 and influence the sensitivity of brown planthopper to buprofezin drugs. The invention lays a foundation for deeper understanding of the relationship between energy metabolism and drug resistance of brown planthopper, and can be used for development of anti-brown planthopper drugs.

The invention discloses miRNA (micro Ribonucleic Acid) for regulating and controlling the diapause behavior of insects and application of the miRNA. The miRNA is aal-miR-137, and the nucleotide sequence of the aal-miR-137 is as shown in SEQ ID NO. 1. The invention also discloses an application of the miRNA in regulation and control of the insect aalClk gene, an application in preparation of products for regulation and control of insect diapause and an application in preparation of biological medium control products. Through high-throughput sequencing, aal-miR-137 is found to be differentially expressed in diapause and non-diapause groups in aedes albopictus and acts on a target gene aalClk, the aalClk participates in rhythm regulation of biological clock genes, then the diapause behavior of seasonal rhythm is regulated and controlled, and the aal-miR-137 has potential application value in diapause regulation and control of aedes albopictus.

This invention relates to the field of nanomaterials and gene interference, specifically disclosing a method for interfering with insect gene expression by infiltrating dsRNA through the insect's body wall. The method involves complexing a nanocarrier with a functional macromolecule of dsRNA, and with the aid of a surfactant, enabling exogenous dsRNA molecules to attach, spread, penetrate the body wall, and enter various organs and tissues within the insect, thus efficiently interfering with gene expression. This invention utilizes the dual synergistic effect of the nanocarrier and the surfactant to achieve efficient and non-invasive delivery of exogenous dsRNA molecules into insect cells, ultimately promoting the correct targeting of dsRNA molecules and improving RNA interference efficiency. The method described in this invention is simple, efficient, and has low error, and has significant practical implications for scientific research on insect gene interference and the application of highly efficient, safe, and targeted RNA pesticide formulations.

The present invention provides a Lepidoptera insect Caspase-5 gene and its application in enhancing the insecticidal virulence of baculovirus against Lepidoptera pests. The nucleotide sequence of the open reading frame of the gene is SEQ ID NO:1, and the amino acid sequence of the encoded protein is SEQ ID NO:2. The screened SeCaspase-5 of the present invention has an apoptosis-promoting function and can enhance the insecticidal virulence of viral polyhedron OB against Spodoptera exigua. Therefore, by constructing a recombinant virus bacmid carrying the SeCaspase-5 gene and transfecting insect cells, the obtained progeny virus polyhedron OB is further fed to Spodoptera exigua larvae, which can enhance the insecticidal virulence of the recombinant virus against pests.

The invention is suitable for the technical field of insect genetic engineering, and provides a holotrichia parallela HparGR16 gene and application thereof. The DNA (Deoxyribose Nucleic Acid) sequence of the HparGR16 gene from holotrichia parallela and participating in CO2 recognition is shown as SEQ ID No: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID No: 2. The invention proves that the HparGR16 gene is the CO2 receptor gene of the holotrichia parallela for the first time, and the CO2 recognition process of the holotrichia parallela can be effectively hindered by interfering the expression of the gene or designing, synthesizing and screening a compound capable of inhibiting the protein activity of the gene. Due to the characteristic, the gene can serve as a target to be used for designing and screening prevention and control agents, then novel pest behavior regulating agents, insecticides and the like are developed, efficient and green prevention and control of holotrichia parallela are achieved, and the gene has important application value in the field of underground pest holotrichia parallela prevention and control gene engineering.

This invention is applicable to the field of insect genetic engineering technology and provides... HparGR22 Genes and their application in establishing an in vitro model for phlorizin recognition. HparGR22 The DNA sequence of the gene is shown in SEQ ID No:1, and the encoded amino acid sequence is shown in SEQ ID No:2. This gene is expressed at the highest level in the labial palps of the larvae of the dark-browed scarab beetle. Oocytes injected with the cRNA of this gene showed a significant response to stimulation with different concentrations of phlorizin, clearly demonstrating... HparGR22 The product of gene expression can recognize phlorizin, thus establishing its function as a phlorizin receptor gene for the black-breasted beetle. Given that the phlorizin recognition function of this gene is clearly defined for the first time, this invention provides a basis for its application in establishing an in vitro model of phlorizin recognition, lays the foundation for exploring its potential value in the field of genetic engineering for the control of the black-breasted beetle, and opens up new directions for further research and application of this gene.