Bird's nest oligopeptide composition and preparation method and use thereof

A composition and oligopeptide technology, used in health care products, cosmetics, compositions containing small molecular oligopeptides, in the field of preparation of medicine, to achieve the effect of improving immunity and unique biological functions

Active Publication Date: 2011-06-22
BEIJING INST OF OLIGO PEPTIDE BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the prior art can solve the problem of obtaining bird’s nest peptide chain lengths mainly from dipeptides to pentapeptides through relatively simple and convenient methods and steps, and small molecule nano-oligopeptides whose molecular weight is mainly distributed between 180-1000D.

Method used

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  • Bird's nest oligopeptide composition and preparation method and use thereof
  • Bird's nest oligopeptide composition and preparation method and use thereof
  • Bird's nest oligopeptide composition and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Clean the fresh bird's nest, mix it with deionized water at a ratio of 1:40, and soak until the bird's nest absorbs water completely. The soaking liquid is heated up to 95°C and kept for 15 minutes to denature the protein; Incubate at 55°C for 5 hours; adjust the pH to 1.8 with 10% hydrochloric acid solution after the incubation, and hydrolyze at 90°C for 3 hours; filter the liquid after the incubation, concentrate the filtrate to 1 / 8 of the original liquid, and vacuum freeze-dry to obtain Light yellow low molecular weight bird's nest oligopeptide powder.

[0054] According to the QB / T22492-2008 standard of HPLC analysis method commonly used in this industry to detect the molecular weight of peptides, the molecular weight distribution of the extract was measured, and the Gaussian distribution results were obtained as follows:

[0055]

Embodiment 2

[0057] Clean the fresh bird's nest, mix it with deionized water at a ratio of 1:40, and soak until the bird's nest absorbs water completely. The soaking solution was heated up to 95°C and kept for 15 minutes to denature the protein; after the heat preservation was over, the temperature was lowered to 55°C, and papain (4 million u / g) was added according to 2.3% of the dry weight of the bird's nest to adjust the pH to 5.2. Incubate at 55°C for 5 hours; after the incubation, the liquid is filtered, the filtrate is concentrated to 1 / 8 of the original liquid, and vacuum freeze-dried to obtain a light yellow low molecular weight bird's nest oligopeptide powder.

[0058] As a result of the test, it was found that the composition with the target low-molecular-weight oligopeptide could not be obtained. In addition, it was found that the content of polysaccharides and other nutrients in the extract was low, and there were impurities.

Embodiment 3

[0060] Clean the fresh bird's nest, mix it with deionized water at a ratio of 1:40, and soak until the bird's nest absorbs water completely. The soaking liquid is heated up to 95°C and kept for 15 minutes to denature the protein; Incubate at 55°C for 5 hours; cool down to 40-50°C, add 150,000 u / g neutral protease according to 1%-3% by weight of fruiting body dry weight, adjust pH to 7-7.5, and incubate at 40-50°C for 2-4h ; adjust the pH to 3.0-4.0, add 50,000 u / g pepsin according to 1%-3% by weight of the dry weight of the fruit body, heat up to 50-60°C, and keep for 2-4h; Concentrate to 1 / 8 of the original liquid amount, and carry out vacuum freeze-drying to obtain light yellow bird's nest molecular weight oligopeptide powder.

[0061] As a result of the test, it was found that the composition with the target low-molecular-weight oligopeptide could not be obtained. In addition, it was found that the content of polysaccharides and other nutrients in the extract was low, and ...

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Abstract

The invention provides a method for extracting small molecule oligopeptides from bird's nests by hydrolysis, which is to obtain a small molecular nano oligopeptide containing composition by hydrolyzing the bird's nests through the combination of enzymolysis and acid hydrolysis. The bird's nest oligopeptide composition is characterized in that: the molecular weight of the nano oligopeptides mainlyranges from 180 to 1,000D; preferably, the percentage of Gaussian distribution peak area of the nano oligopeptides with a molecular weight ranging from 180 to 1,000D in the composition is over 40 percent. The Cordyceps militarisoligopeptide extract provided by the invention retain other active ingredients such as polysaccharide, bird's nest sialic acid, micro elements and the like. The invention also relates to the use of the nano oligopeptide containing composition extracted from bird's nests in the preparation of medicines, health-care products and cosmetics.

Description

technical field [0001] The invention relates to a method for extracting oligopeptides from bird's nest and a composition obtained by the method. The invention also relates to the application of the composition containing the small molecular oligopeptide extracted from the bird's nest in the preparation of medicines, health products and cosmetics. Background technique [0002] Bird's nests are several kinds of birds of the Swift family Apodidae swiftlets of the genus Collocalia. After swallowing small fish in the sea or other small organisms such as silkworm snails and seaweeds, the gastric juice secreted and their down feathers are mixed and condensed on the cliffs to build nests. nest. Bird's nest is one of the famous and precious tonics with the same reputation as ginseng and deer antler. [0003] There are mucins in bird's nest, which include sixteen kinds of amino acids necessary for the human body, as well as active ingredients such as sialic acid and trace elements. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04A23L1/305A61K38/03A61K8/64
Inventor 胡如桂杜以波
Owner BEIJING INST OF OLIGO PEPTIDE BIOLOGY
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