Composite prebiotics with intestinal tract improving function and preparation method and application thereof

Abstract

The invention belongs to the technical field of medicines and health-care products, and particularly relates to compound prebiotics with an intestinal tract improving function as well as a preparation method and application of the compound prebiotics. The compound prebiotics with the function of improving the intestinal tract, disclosed by the invention, are prepared from the following components in parts by mass: 2 to 5 parts of polydextrose, 1 to 2 parts of erythritol, 8 to 10 parts of oat beta-glucan, 4 to 6 parts of stachyose, 12 to 15 parts of beta-galactomannan oligosaccharide, 20 to 30 parts of isomaltooligosacharide and 2 to 4 parts of a multifunctional additive. The multifunctional additive comprises fructo-oligosaccharide, red wine polyphenol and fatty acid. The compound prebiotics provided by the invention not only can promote the growth and reproduction of probiotics, improve the micro-ecological balance in intestinal tracts, improve the immunity of the intestinal tracts and improve the functions of the intestinal tracts, but also can prevent diarrhea.

Description

Technical Field

[0001] The invention belongs to the technical field of medicines and health products, and specifically relates to a composite prebiotic capable of improving intestinal function, and a preparation method and application thereof. Background Art

[0002] The intestine is one of the most important digestive tracts in the human body, providing the body with the nutrients it needs. The four major barrier functions of the intestine refer to the protective effects of the intestine against harmful microorganisms, toxins, and other harmful substances. These barriers include the following four aspects: (1) Physiological barrier: The intestinal mucus layer and natural bactericidal substances produced by the intestinal wall (such as digestive enzymes and bile) can prevent harmful bacteria and toxins from entering the body. (2) Immune barrier: There are a large number of immune cells in the intestine that can identify and attack harmful microorganisms and toxins to prevent them from entering the body through the intestine. (3) Ecological barrier: The normal flora in the intestine can inhibit the growth and reproduction of pathogenic microorganisms, maintain the ecological balance of intestinal microorganisms, and protect the intestine from invasion by harmful microorganisms. (4) Barrier repair: When the intestine is injured or damaged, intestinal cells can re-establish the intestinal barrier through the mechanism of cell regeneration and repair to prevent harmful substances from penetrating into the body. The four major barrier functions of the intestine are important factors in maintaining human health. By maintaining the integrity and function of the intestinal barrier, many diseases can be prevented and the health of the body can be promoted. There are many factors that damage the intestinal mucosal barrier. When one or more destructive factors act together or successively on the intestinal mucosa, the homeostasis of the intestinal mucosa is easily damaged. The interaction between the host and the intestinal flora is complex. The host provides nutrients and environment for the growth and reproduction of normal microbial communities, and the intestinal flora, as a "microbial organ" in the gastrointestinal tract, plays a key role in the health, metabolism and homeostasis of the body. Under normal circumstances, the intestinal flora of healthy people is balanced. However, due to changes in the types, numbers and functions of microorganisms, the negative impact of internal and external environments on the state of the human body will lead to an imbalance in the intestinal flora. At the same time, the composition of the intestinal flora also affects the state of the host. Therefore, the intestinal flora plays an important role in maintaining human health.

[0003] In 1995, the concept of prebiotics was first proposed. Prebiotics refer to a class of bacteria that can selectively stimulate the growth and / or activity of specific types or colonies of bacteria in the colon and have a beneficial effect on the host, thereby improving the health of the host. With the rapid advancement of science and technology and the deepening of research, the definition of prebiotics was redefined in 2008 as "a selective fermentation ingredient that can cause specific changes in the imbalance of gastrointestinal microecology locally or as a whole to improve the health of the host." The International Scientific Association of Probiotics and Prebiotics (ISAPP) re-proposed a new definition of prebiotics in 2016, describing it as a substrate with health benefits that is selectively utilized by host microorganisms. There are a large number of microorganisms in the human intestine, which form a symbiotic relationship with humans. The intestinal flora is vital to human health and is called an "invisible organ." When the human intestinal flora is unbalanced, it may cause a variety of diseases, such as allergies, irritable bowel syndrome and depression. Prebiotics can restore the balance of the human intestinal flora by proliferating beneficial bacteria and inhibiting the growth and activity of harmful bacteria. At present, there are many studies on prebiotics. For example, Chinese patent application CN100571521A discloses a liquid milk containing prebiotics. Each 100g contains the following components by weight: 50-95g milk, 0.01-10.0g isomaltooligosaccharide, 0.01-0.7g xylooligosaccharide, 0.01-5.0g fructooligosaccharide, 0.1-1.0g stabilizer, and water is added to 100g. CN117941765A discloses a method for preparing a plurality of prebiotic tablet candies, S101, sieving inulin, oligofructose, oligoxylose, cottonseed oligosaccharide, sorbitol and resistant dextrin to remove unqualified particles; S102, mixing the finely sieved inulin, oligofructose, oligoxylose, cottonseed oligosaccharide, sorbitol and resistant dextrin to obtain a mixture; S103, adding a binder to the mixture, drying the whole particles to obtain a semi-finished product A. However, the above prebiotics have the problem of poor improvement of intestinal function, and prebiotics can stimulate intestinal peristalsis or increase intestinal sensitivity to cause diarrhea. Summary of the invention

[0004] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a composite prebiotic that improves intestinal function and its preparation method and application. The composite prebiotic provided by the present invention can not only promote the growth and reproduction of probiotics, improve the microecological balance in the intestine, enhance the immunity of the intestine, improve intestinal function, but also prevent the occurrence of diarrhea.

[0005] The technical solution of the present invention is:

[0006] A composite prebiotic for improving intestinal function, comprising the following components and their weight proportions: 2-5 parts of polydextrose, 1-2 parts of erythritol, 8-10 parts of oat beta-glucan, 4-6 parts of stachyose, 12-15 parts of beta-galactomannan oligosaccharide, 20-30 parts of isomaltooligosaccharide, and 2-4 parts of a multifunctional additive;

[0007] The multifunctional additive comprises oligofructose, red wine polyphenol and fatty acid.

[0008] Due to the influence of factors such as different degrees of polymerization and chemical structures of different prebiotics, the types of probiotics proliferated by different prebiotics are also different, the fermentation rates and intestinal fermentation sites of different prebiotics are different, and different effects are produced on the proliferation of different probiotics. As for intestinal probiotics, their utilization of prebiotics is also different, so when different prebiotics are used simultaneously, synergistic or antagonistic effects may occur. The present invention has found through a large number of creative experiments that the reasonable combination of polydextrose, erythritol, oat β-glucan, stachyose, β-galactomannan oligosaccharide and oligosaccharide isomaltose can not only promote the growth and reproduction of probiotics, improve the microecological balance in the intestine, but also increase the amount of short-chain fatty acids (acetic acid, propionic acid, butyric acid) produced by intestinal bacterial fermentation, improve the immunity of the intestine, and improve intestinal function.

[0009] Furthermore, the composite prebiotic for improving intestinal function comprises the following components and their mass fractions: 3 parts of polydextrose, 1.2 parts of erythritol, 9 parts of oat β-glucan, 5 parts of stachyose, 13 parts of β-galactomannan oligosaccharide, 26 parts of isomaltooligosaccharide, and 3 parts of multifunctional additive.

[0010] Furthermore, the multifunctional additive is composed of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 15-20:5-8:1-3.

[0011] Furthermore, the multifunctional additive consists of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 16:7:2.

[0012] Furthermore, the fatty acid is composed of linoleic acid and arachidonic acid in a mass ratio of 8-13:2-4.

[0013] Furthermore, the fatty acid consists of linoleic acid and arachidonic acid in a mass ratio of 11:3.

[0014] Although prebiotics can promote the growth and reproduction of probiotics, improve the microecological balance in the intestine, enhance the immunity of the intestine, and improve intestinal function, prebiotics can stimulate intestinal peristalsis to accelerate or cause increased intestinal sensitivity and lead to diarrhea. The multifunctional additive composed of oligofructose, red wine polyphenols and fatty acids added by the present invention can not only further enhance the effect of prebiotics on improving intestinal function, but also the fatty acids composed of linoleic acid and arachidonic acid can promote the growth and adhesion function of Lactobacillus casei and prevent the occurrence of diarrhea.

[0015] The present invention also provides a method for preparing the composite prebiotics capable of improving intestinal function, comprising the following steps:

[0016] Polydextrose, erythritol, oat β-glucan, stachyose, β-galactomanno oligosaccharide and isomaltooligosaccharide are mixed, and the mixture is sieved through a 60-100 mesh sieve to obtain a mixed powder. A multifunctional additive is added to the mixed powder, and the mixture is stirred and dried to obtain the product.

[0017] Furthermore, the stirring speed is 50-80 r / min, and the stirring time is 15-25 min.

[0018] Another object of the present invention is to provide the use of the composite prebiotics having the function of improving intestinal function in the preparation of medicines or health products having the function of improving intestinal function.

[0019] Compared with the prior art, the present invention has the following advantages:

[0020] (1) The composite prebiotics provided by the present invention can not only promote the growth and reproduction of probiotics and improve the microecological balance in the intestine, but also prevent the occurrence of diarrhea.

[0021] (2) The composite prebiotics provided by the present invention are highly safe and have no toxic side effects.

[0022] (3) The preparation method of the composite prebiotic provided by the present invention is simple and the raw materials are easily available.

[0023] (4) Short-chain fatty acids can not only regulate immune cell differentiation and immune response, but also play an important role in protecting the intestinal mucosal barrier function. The composite prebiotics provided by the present invention can increase the amount of short-chain fatty acids (acetic acid, propionic acid, butyric acid) produced by intestinal bacterial fermentation, improve intestinal immunity, and improve intestinal function. DETAILED DESCRIPTION

[0024] The present invention is further illustrated below through the description of specific implementation modes, but this is not a limitation of the present invention. Those skilled in the art can make various modifications or improvements based on the basic idea of ​​the present invention, but as long as they do not deviate from the basic idea of ​​the present invention, they are all within the scope of the present invention.

[0025] Unless otherwise specified, the raw materials used in the present invention are all commercially available. For example, polydextrose can be purchased from Shandong Bailong Chuangyuan Biotechnology Co., Ltd.; oat β-glucan can be purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., item number: TGYSW-4286900; β-galactomannan oligosaccharide can be purchased from Zhengzhou Best Food Additive Co., Ltd.; isomaltooligosaccharide (C 12 H 22 O 11 ) can be purchased from Shenzhen Jinfuyuan Biotechnology Co., Ltd.; oligofructose can be purchased from Xi'an Lavia Biotechnology Co., Ltd.; galacto-oligosaccharide can be purchased from Shandong Bailong Chuangyuan Biotechnology Co., Ltd.; red wine polyphenols can be purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., item number: TGYHJDF-4151; tea polyphenols can be purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., item number: TGYCDF-5115.

[0026] Example 1: A composite prebiotic for improving intestinal function

[0027] The composite prebiotic with the function of improving intestinal function comprises the following components and their mass fractions: 2 parts of polydextrose, 1 part of erythritol, 8 parts of oat β-glucan, 4 parts of stachyose, 12 parts of β-galactomannan oligosaccharide, 20 parts of isomaltooligosaccharide, and 2 parts of a multifunctional additive; the multifunctional additive is composed of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 15:8:3; the fatty acids are composed of linoleic acid and arachidonic acid in a mass ratio of 8:4.

[0028] The method for preparing the composite prebiotics capable of improving intestinal function comprises the following steps:

[0029] Mix polydextrose, erythritol, oat β-glucan, stachyose, β-galactomanno oligosaccharide and isomaltooligosaccharide, pass through a 60-mesh sieve to obtain a mixed powder, add a multifunctional additive to the obtained mixed powder, stir at a speed of 50 r / min for 15 min, and dry to obtain the product.

[0030] Example 2: A composite prebiotic for improving intestinal function

[0031] The composite prebiotic with improved intestinal function comprises the following components and their mass fractions: 5 parts of polydextrose, 2 parts of erythritol, 10 parts of oat β-glucan, 6 parts of stachyose, 15 parts of β-galactomannan oligosaccharide, 30 parts of isomaltooligosaccharide, and 4 parts of a multifunctional additive; the multifunctional additive is composed of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 20:5:1; the fatty acids are composed of linoleic acid and arachidonic acid in a mass ratio of 13:2.

[0032] The method for preparing the composite prebiotics capable of improving intestinal function comprises the following steps:

[0033] Mix polydextrose, erythritol, oat β-glucan, stachyose, β-galactomanno oligosaccharide and isomaltooligosaccharide, pass through a 100-mesh sieve to obtain a mixed powder, add a multifunctional additive to the obtained mixed powder, stir at a speed of 80 r / min for 25 min, and dry to obtain the product.

[0034] Example 3: A composite prebiotic for improving intestinal function

[0035] The composite prebiotic with improved intestinal function comprises the following components and their mass fractions: 3 parts of polydextrose, 1.2 parts of erythritol, 9 parts of oat beta-glucan, 5 parts of stachyose, 13 parts of beta-galactomanno oligosaccharide, 26 parts of isomaltooligosaccharide, and 3 parts of a multifunctional additive; the multifunctional additive is composed of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 16:7:2; the fatty acids are composed of linoleic acid and arachidonic acid in a mass ratio of 11:3.

[0036] The method for preparing the composite prebiotics capable of improving intestinal function comprises the following steps:

[0037] Mix polydextrose, erythritol, oat β-glucan, stachyose, β-galactomanno oligosaccharide and isomaltooligosaccharide, pass through an 80-mesh sieve to obtain a mixed powder, add a multifunctional additive to the obtained mixed powder, stir at a speed of 60 r / min for 20 min, and dry to obtain the product.

[0038] Comparative Example 1: A composite prebiotic for improving intestinal function

[0039] The composite prebiotic with improved intestinal function comprises the following components and their mass fractions: 3 parts of polydextrose, 1.2 parts of erythritol, 9 parts of oat beta-glucan, 5 parts of stachyose, 13 parts of galacto-oligosaccharide, 26 parts of isomaltose, and 3 parts of a multifunctional additive; the multifunctional additive is composed of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 16:7:2; the fatty acids are composed of linoleic acid and arachidonic acid in a mass ratio of 11:3.

[0040] The preparation method of the composite prebiotics for improving intestinal function is similar to that of Example 3;

[0041] The difference from Example 3 is that β-galactomanno oligosaccharide is replaced by galacto-oligosaccharide.

[0042] Comparative Example 2: A composite prebiotic for improving intestinal function

[0043] The composite prebiotic for improving intestinal function comprises the following components and their mass fractions: 3 parts of polydextrose, 1.2 parts of erythritol, 9 parts of oat beta-glucan, 5 parts of stachyose, 13 parts of beta-galactomanno oligosaccharide, 26 parts of isomaltooligosaccharide, and 3 parts of a multifunctional additive; the multifunctional additive is composed of oligofructose, tea polyphenols and fatty acids in a mass ratio of 16:7:2; the fatty acids are composed of linoleic acid and arachidonic acid in a mass ratio of 11:3.

[0044] The preparation method of the composite prebiotics for improving intestinal function is similar to that of Example 3;

[0045] The difference from Example 3 is that the multifunctional additive consists of oligofructose, tea polyphenols and fatty acids in a mass ratio of 16:7:2.

[0046] Comparative Example 3: A composite prebiotic for improving intestinal function

[0047] The composite prebiotic with improved intestinal function comprises the following components and their mass fractions: 3 parts of polydextrose, 1.2 parts of erythritol, 9 parts of oat beta-glucan, 5 parts of stachyose, 13 parts of beta-galactomanno oligosaccharide, 26 parts of isomaltooligosaccharide, and 3 parts of a multifunctional additive; the multifunctional additive is composed of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 16:7:2; the fatty acids are composed of linoleic acid and docosahexaenoic acid in a mass ratio of 11:3.

[0048] The preparation method of the composite prebiotics for improving intestinal function is similar to that of Example 3;

[0049] The difference from Example 3 is that the fatty acid consists of linoleic acid and docosahexaenoic acid in a mass ratio of 11:3.

[0050] Test Example 1: Experiment on improving intestinal function

[0051] 1. Test materials: composite prebiotics for improving intestinal function prepared in Example 1, Example 2, Example 3, Comparative Example 1 and Comparative Example 2.

[0052] 2. Experimental subjects: Kunming mice, 2 months old, weighing 20 g, a total of 70 mice, half male and half female.

[0053] 3. Test methods:

[0054] The mice were raised under standard conditions (25±2)°C, relative humidity (60±5)% and 12h light / 12h dark cycle), and free access to food and water. After 7 days, the mice were randomly divided into a blank control group, a model group, an embodiment 1 group, an embodiment 2 group, an embodiment 3 group, a comparative example 1 group, and a comparative example 2 group, each group having 10 mice, half male and half female, the blank control group was fed with a common feed containing 10% of fat calories, the model group, the embodiment 1 group, the embodiment 2 group, the embodiment 3 group, the comparative example 1 group, and the comparative example 2 group were fed with a high-fat feed containing 60% of fat calories, and the feeding was continued for 9 weeks to construct an intestinal barrier injury model.

[0055] After the intestinal barrier injury model was successfully established, the mice in the blank control group and the model group were gavaged with sterile saline every day, at a volume of 0.1 mL / 10 g; the composite prebiotics prepared by Example 1, Example 2, Example 3, Comparative Example 1, and Comparative Example 2 were gavaged daily, respectively, at 0.03 g / kg BW, for 7 weeks. During the experiment, the blank control group was fed with ordinary feed, which contained 10% fat calories, and the model group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, and Comparative Example 2 group were fed with high-fat feed, which contained 60% fat calories. The weight of the mice was monitored and recorded once a week. After the experiment, the mice were anesthetized, killed by cervical dislocation, and the colon contents were collected, weighed, and then quickly frozen in liquid nitrogen and stored at -80 ° C for standby use. The concentration of short-chain fatty acids in the colon contents was determined by gas chromatography-mass spectrometry.

[0056] 4. Experimental results: The concentrations of short-chain fatty acids in the colon contents of different groups are shown in Table 1.

[0057] Table 1: Concentrations of short-chain fatty acids in colonic contents of different groups

[0058] Group Acetic acid (μg / g) Propionic acid (μg / g) Butyric acid (μg / g) Blank control group 395.28 802.45 756.17 Model Group 287.1 571.56 603.84 Example 1 Group 405.73 781.6 773.29 Example 2 Group 578.62 833.74 869.63 Example 3 Group 665.81 908.19 1025.87 Comparative Example 1 368.37 736.75 734.09 Comparative Example 2 342.15 684.32 652.43

[0059] As can be seen from Table 1, compared with the blank control group, the concentrations of acetic acid, propionic acid and butyric acid in the colon contents of the mice in the model group were significantly reduced, indicating that the modeling was successful. Compared with the model group, the concentrations of acetic acid, propionic acid and butyric acid in the colon contents of the mice in Example 1, Example 2 and Example 3 groups were greatly increased, among which the concentrations of acetic acid, propionic acid and butyric acid in the mice in Example 3 group, which were intragastrically administered with the composite prebiotics with improved intestinal function prepared in Example 3 of the present invention, were the most significant, and significantly better than the blank control group. It can be seen that the composite prebiotics with improved intestinal function of the present invention can promote the production of short-chain fatty acids, which is beneficial to the protection of the intestinal mucosal barrier and other effects.

[0060] Test Example 2: Diarrhea Prevention Test

[0061] 1. Test materials: the composite prebiotics for improving intestinal function prepared in Example 3 and Comparative Example 3.

[0062] 2. Experimental subjects: 40 Kunming mice, 2 months old, weighing 20 g, half male and half female.

[0063] 3. Test methods:

[0064] The mice were randomly divided into a blank control group, a model group, an embodiment 3 group, and a comparative example 3 group, with 10 mice in each group, half male and half female. The blank control group did not build a model, and the other groups were built using the following method: ampicillin + streptomycin + clindamycin, the dose was 700 + 790 + 395 mg / kg, dissolved or suspended in saline, and administered by gavage for 14 consecutive days. After the animal diarrhea model was successfully established, the blank control group was continuously gavaged with sterile saline every day, at 0.1 mL / 10 g; the model group was continuously gavaged with saline every day, at 0.1 mL / 10 g; the embodiment 3 group and the comparative example 3 group were respectively continuously gavaged with the composite prebiotics with improved intestinal function prepared in the embodiment 3 and the comparative example 3 every day, at 0.03 g / kg BW, and the feces dry-wet weight ratio of each group was recorded until the mice recovered.

[0065] 4. Test results: The results of the diarrhea prevention test are shown in Table 2.

[0066] Table 2: Results of the diarrhea prevention experiment

[0067]

[0068] As can be seen from Table 2, on day 0, the feces dry-wet weight ratio of mice in the model group, Example 3 group, and Comparative Example 3 group was significantly lower than that of the blank control group, indicating that the modeling was successful. After intragastric administration of the composite prebiotics with improved intestinal function prepared in Example 3 of the present invention for 4 days, the feces dry-wet weight ratio of mice in the Example 3 group was close to that of the blank control group, indicating that the diarrheal mice were cured. However, the mice that were intragastrically administered with the composite prebiotics with improved intestinal function prepared in Comparative Example 3 were cured only on the 6th day. It can be seen that the composite prebiotics with improved intestinal function prepared in the present invention has the effect of preventing diarrhea.

[0069] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A composite prebiotic for improving intestinal function, characterized in that: The invention comprises the following components and their weight proportions: 2-5 parts of polydextrose, 1-2 parts of erythritol, 8-10 parts of oat beta-glucan, 4-6 parts of stachyose, 12-15 parts of beta-galactomannan oligosaccharide, 20-30 parts of isomaltooligosaccharide, and 2-4 parts of multifunctional additives; The multifunctional additive comprises oligofructose, red wine polyphenol and fatty acid.

2. The composite prebiotic for improving intestinal function according to claim 1, characterized in that: The invention comprises the following components and their weight proportions: 3 parts of polydextrose, 1.2 parts of erythritol, 9 parts of oat beta-glucan, 5 parts of stachyose, 13 parts of beta-galactomannan oligosaccharide, 26 parts of isomaltooligosaccharide and 3 parts of multifunctional additive.

3. The composite prebiotic for improving intestinal function according to claim 1 or 2, characterized in that: The multifunctional additive consists of oligofructose, red wine polyphenol and fatty acid in a mass ratio of 15-20:5-8:1-3.

4. The composite prebiotic for improving intestinal function according to claim 3, characterized in that: The multifunctional additive consists of oligofructose, red wine polyphenols and fatty acids in a mass ratio of 16:7:

2.

5. The composite prebiotic for improving intestinal function according to claim 1 or 2, characterized in that: The fatty acid is composed of linoleic acid and arachidonic acid in a mass ratio of 8-13:2-4.

6. The composite prebiotic for improving intestinal function according to claim 5, characterized in that: The fatty acid consists of linoleic acid and arachidonic acid in a mass ratio of 11:

3.

7. The method for preparing the composite prebiotic for improving intestinal function according to any one of claims 1 to 6, characterized in that: The following steps are involved: Polydextrose, erythritol, oat β-glucan, stachyose, β-galactomanno oligosaccharide and isomaltooligosaccharide are mixed, and the mixture is sieved through a 60-100 mesh sieve to obtain a mixed powder. A multifunctional additive is added to the mixed powder, and the mixture is stirred and dried to obtain the product.

8. The method for preparing the composite prebiotic for improving intestinal function according to claim 7, characterized in that: The stirring speed is 50-80r / min and the stirring time is 15-25min.

9. Use of the composite prebiotic having the function of improving intestinal function as claimed in any one of claims 1 to 6 in the preparation of a medicine or health product having the function of improving intestinal function.

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