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Method for producing high-concentration reduced sugar by combining enzymatic hydrolysis of lignocellulose and membrane separation

A lignocellulase, lignocellulose technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve problems such as unfavorable, low reducing sugar concentration, unfavorable microbial fermentation, etc. The effect of continuity, easy automation and industrialization

Inactive Publication Date: 2010-12-01
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the enzyme-membrane reactors reported in the literature are built-in, which is not conducive to industrial scale-up, and has the disadvantage of low reducing sugar concentration in the permeate, which is not conducive to subsequent microbial fermentation.

Method used

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  • Method for producing high-concentration reduced sugar by combining enzymatic hydrolysis of lignocellulose and membrane separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: A kind of method that lignocellulosic enzymatic hydrolysis and membrane separation are coupled to produce high-concentration reducing sugars comprises the following steps:

[0021] (1) add dilute acid-treated bagasse, cellulase and β-glucosidase in the enzymolysis reactor in a ratio of 5% (w / v), and the consumption of cellulase and β-glucosidase is 20IU respectively / g substrate and 20IU / g substrate. The pH was adjusted to 4.8, and incubated at 50°C for 1 day.

[0022] (2) Turn on the feed pump, pump the enzymolysis solution into the microfiltration module (polyvinylidene fluoride (PVDF) membrane), and return the retained lignocellulose substrate to the enzymolysis reactor. The pore size of the microfiltration membrane is about 0.45 microns, and the pressure inside the module is 0.1-0.2 MPa.

[0023] (3) The permeate obtained in step (2) enters the tubular polyamide ultrafiltration module with a molecular weight cut-off of 10,000 Daltons, and the retaine...

Embodiment 2

[0025] Embodiment 2: A kind of method that lignocellulosic enzymatic hydrolysis and membrane separation are coupled to produce high-concentration reducing sugars comprises the following steps:

[0026] (1) Add poplar sawdust, cellulase and β-glucosidase treated with dilute alkali in the ratio of 3% (w / v) in the enzymolysis reactor, and the consumption of cellulase and β-glucosidase is respectively 10IU / g substrate and 20IU / g substrate. The pH was adjusted to 4.8, and incubated at 50°C for 1 day.

[0027] (2) Turn on the feed pump, pump the enzymolysis solution into the microfiltration module (PVDF membrane), and return the retained lignocellulose substrate to the enzymolysis reactor. The pore size of the microfiltration membrane is about 0.45 microns, and the pressure inside the module is 0.1-0.2 MPa.

[0028] (3) The permeate obtained in step (2) enters the flat polyethersulfone ultrafiltration module with a molecular weight cut-off of 5000 Daltons, and the retained hydrola...

Embodiment 3

[0030] Embodiment 3: a kind of method for coupling lignocellulosic enzymatic hydrolysis and membrane separation to produce high-concentration reducing sugars, comprising the following steps:

[0031] (1) Add steam-exploded wheat straw, cellulase, β-glucosidase and xylanase in the enzymolysis reactor in a ratio of 10% (w / v), cellulase, β-glucoside The enzyme and xylanase dosages were 40 IU / g substrate, 20 IU / g substrate and 20 IU / g substrate, respectively. The pH was adjusted to 4.8, and incubated at 50°C for 1 day.

[0032] (2) Turn on the feed pump, pump the enzymolysis solution into the microfiltration module (ceramic membrane), and return the retained lignocellulose substrate to the enzymolysis reactor. The pore size of the microfiltration membrane is about 0.22 microns, and the pressure inside the module is 0.1-0.2 MPa.

[0033] (3) The permeate obtained in step (2) enters the hollow fiber polyethersulfone ultrafiltration module with a molecular weight cut-off of 10,000 ...

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Abstract

The invention relates to a method for producing a high-concentration reduced sugar by combining enzymatic hydrolysis of lignocellulose and membrane separation. In the method, a lignocellulose substrate, a hydrolytic enzyme and a reduced sugar hydrolysate in enzymolysis solution are separated through microfiltration and ultrafiltration, the repeated use of the substrate and the enzyme is realized, the enzymolysis solution is concentrated by nanofiltering, and thus, the solution of the high-concentration reduced sugar is prepared. In the method, due to the combination of microfiltration, ultrafiltration and nanofiltration, the contamination of the lignocellulose substrate to an ultrafiltration membrane is effectively reduced, the inhibiting effect of the hydrolysate on enzymatic hydrolysis of the lignocellulose is avoided, the enzymatic efficiency is improved, the cost of enzymolysis is reduced, energy and water in a production process are saved, the concentration of the reduced sugar in the enzymolysis solution is increased, and the production of a high-concentration product through microbial fermentation is easy.

Description

field of invention [0001] The invention relates to a method for refining sugar in lignocellulose enzymatic hydrolysis liquid, in particular to a method for producing high-concentration reducing sugar by coupling membrane separation technology with lignocellulose enzymatic hydrolysis. Background technique [0002] Lignocellulose is a renewable resource with a wide range of sources and low price in nature, and its main components are cellulose, hemicellulose and lignin. Bioconversion of sugar polymers in lignocellulose to produce biofuels or bio-based chemicals is an effective way for comprehensive utilization of lignocellulose. The lignocellulose bioconversion process includes four steps: pretreatment, enzymatic hydrolysis, microbial fermentation and product separation, among which enzymatic hydrolysis is a very important step. Currently, the cost of enzymes to hydrolyze lignocellulose is high. Therefore, after enzymatic hydrolysis, recycling and utilizing hydrolytic enzyme...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02C07H1/06
CPCY02P20/582
Inventor 万印华齐本坤陈向荣伊守亮苏仪沈飞
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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