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Microbial L. rhamnosus GM-020 and its use for treating obesity

A technology of Lactobacillus rhamnosus and microorganisms, applied in the direction of medical raw materials derived from bacteria, medical preparations containing active ingredients, bacteria, etc., can solve the problem of unobserved weight changes

Active Publication Date: 2005-09-21
GENMONT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, no changes in body weight were observed when known strains of L. rhamnosus were administered

Method used

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  • Microbial L. rhamnosus GM-020 and its use for treating obesity
  • Microbial L. rhamnosus GM-020 and its use for treating obesity
  • Microbial L. rhamnosus GM-020 and its use for treating obesity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Isolation of Lactobacillus rhamnosus GM-020

[0060] A piece of human stomach tissue taken out through an endoscope was cultured in 2 mL of Lactobacillus MRS medium (DIFCO(R) 0881). The medium containing the tissue was laid flat on the selective agar of Lactobacillus and incubated at 37°C for one day. A single colony grown on the culture plate was selected and subjected to Gram staining. Then select Gram Yang bacteria. A strain was cloned, which was called Lactobacillus rhamnosus GM-020.

Embodiment 2

[0061] Example 2 Cell wall protein extraction and analysis of GM-020

[0062] The 24-hour-old cells of Lactobacillus mesophila were harvested in MRS medium (Difco®), and the cells contained 0.1M CaCl 2 Rinse twice in 0.05M Tris-HCl (pH 7.5), and resuspend in A 600 10.0 in 1ml of the same buffer. After centrifugation at 8,000×g for 5 minutes, 1.0ml extraction buffer (pH 8.0) containing 0.01M EDTA, 0.01M NaCl, and 2% (wt / vol) SDS can be obtained from these pellets ( pellet) to extract cell wall proteins. The suspension was stored for 60 minutes at room temperature, heated at 100°C for 5 minutes, and centrifuged at 11,600×g for 10 minutes at 4°C. The supernatant was analyzed by 12% SDS-PAGE and stained with Comassie blue.

Embodiment 3

[0063] Example 3 2-D total protein electrophoresis of GM-020

[0064] Along with 0.5 mL of dissolution buffer C (7M urea, 4% CHAPS, 2M thiourea, 40 mM tris, 0.5% IPG buffer and 5 mM TBP) and 100 L glass beads, 10 mg GM-020 was added. Then, the solution was subjected to ultrasonic degradation treatment and centrifuged at 10,000 rpm for 30 minutes. Available through Bio-rad PlusOne TMProtein assay to determine total protein, and then subject to 2-D electrophoresis with a pH of 3 to 10 IEF. The electrophoresis as designed in Table 5 was performed for 4 hours with a 10% gel and 40 mA / gel. Then the gel was fixed for 30 minutes with 10% methanol and 7% acetic acid. After fixing, the gel was stained with sypro ruby ​​stain for 5 hours, and then the gel was decolorized with 10% methanol and 7% acetic acid for 6 hours. The result is like image 3 Shown.

[0065] 30V

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PUM

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Abstract

The invention provides a strain of separated microbiological isodulcitol lactobacillus GM-020, it is found that the Lactobacillus rhamnosus GM-020 can be used for effectively treating obesity and its complication. The invention also provides the use of Lactobacillus rhamnosus GM-020 for treating obesity and its complication.

Description

Technical field [0001] The present invention mainly relates to a new strain of microorganism Lactobacillus rhamnosus GM-020 and its use in treating obesity or its complications. Background technique [0002] Obesity is usually caused by excess body fat due to changes in physiological or biochemical functions, and it can significantly damage health. Fats generally include neutral lipids, phospholipids and cholesterol. The increase in weight depends on a person's energy intake greater than energy expenditure. There are two types of obesity: (a) simple obesity and (b) second obesity. Simple obesity can be divided into congenital obesity (idiopathic obesity) and acquired obesity (acquired obesity), which can account for more than 95% of obesity. Congenital obesity is caused by a large number of fat cells and is common in childhood obesity. Acquired obesity is caused by larger fat cells and is common in adult obesity. Secondary obesity is also called symptomatic obesity, which is usua...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P3/04C12N1/20
Inventor 许清祥苏伟志
Owner GENMONT BIOTECH
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