Method for preparing edible mushroom synbiotics microcapsule
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A technology of microcapsules and edible fungi, applied in the field of bioengineering, can solve the problems of reduced proliferation and colonization, waste of probiotics, etc.
Inactive Publication Date: 2009-04-08
NORTHEAST AGRICULTURAL UNIVERSITY
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[0002] Microcapsule technology has long been widely used in medicine, chemical industry, food and other fields. With the continuous development and improvement of probiotic microencapsulation technology, probiotic microcapsules have excellent acid resistance, bile salt resistance, storage resistance and enteric dissolution. Although the probiotics are fully protected, new problems arise one after another: after the probiotics reach the intestinal tract, their proliferat
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Embodiment 1
[0030] The 4 strains of probiotics used in the present invention were all purchased from China Industrial Microorganism Culture Collection Center, respectively: Lactobacillus acidophilus CICC 6074, Lactobacillus plantarum CICC 6002, Lactobacillus casei CICC20284, Lactobacillus rhamnosus CICC6001, in the present invention The above bacterial strains are referred to as bacteria A, bacteria B, bacteria C, and bacteria D for short.
[0031] 1. Selection of probiotics and preparation of medium
[0032] Bacteria A——Lactobacillus acidophilus CICC6074 (Lactobacillus acidophilus), medium: 10 grams of peptone, 10 grams of beef extract, 5 grams of yeast extract, 20 grams of glucose, 2 grams of dipotassium hydrogen phosphate, 5 grams of sodium acetate, lemon 2 grams of ammonium sulfate, 0.2 grams of magnesium sulfate, 0.2 grams of manganese sulfate, 801 ml of Tween, 1000 ml of water, pH 6.2-6.6.
[0033] B bacteria - Lactobacillus plantarum CICC6002 (Lactobacillus plantarum) 5 ° Bé wort,...
Embodiment 2
[0049] 1. Selection of probiotics and preparation of medium
[0050] Bacteria A——Lactobacillus acidophilus CICC6074 (Lactobacillus acidophilus), medium: 10 grams of peptone, 10 grams of beef extract, 5 grams of yeast extract, 20 grams of glucose, 2 grams of dipotassium hydrogen phosphate, 5 grams of sodium acetate, lemon 2 grams of ammonium sulfate, 0.2 grams of magnesium sulfate, 0.2 grams of manganese sulfate, 801 ml of Tween, 1000 ml of water, pH 6.2-6.6.
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Abstract
The invention provides a method for preparing edible bacteria synbiotic microcapsules. The method comprises the following steps: firstly, zymotic fluids of four strains of probiotic bacteria, namely Lactobacillus acidophilus, lactobacillus plantarum, lactobacillus casei and lactobacillus rhamnosus are prepared, then supernatants are removed after the speed of 5000r/min, and bacterial sludges are embedded for standby. 10g of each bacterial sludge of the probiotic bacteria to be embedded and 4g of edible bacteria Agaricus blazei powder with the fineness of 200 meshes are mixed with 40g of mixed colloid containing 2.5 percent of sodium alginate and 0.5 percent of flaxseed gum respectively; the first stirring is performed for 20min at a rotating speed of 200 r/min; the mixture is poured into a triangular flask which contains 1.5 times of volume of edible oil; the second stirring is performed for 20min at a rotating speed of 400r/min; a 1.5 percent CaCl2 solution is added into the mixture to be calcified for 3h; sterilized physiological saline is used to wash gel beads for three times; the gel beads are placed into a 0.6 percent chitosan solution with a pH value of 5.5 to perform a film-forming reaction for 15min, taken out, washed by the sterilized physiological saline and then placed into a citric acid solution with the concentration of 55mM to be liquefied for 30min; and wet microcapsules are obtained after the washing of the sterilized physiological saline, collected by centrifugating for 10min at a rotating speed of 150r/min, precooled, and finally freeze-dried to obtain the microcapsules.
Description
(1) Technical field [0001] The invention relates to a preparation method of edible fungus synbiotic microcapsules, belonging to the field of bioengineering. (2) Background technology [0002] Microcapsule technology has long been widely used in medicine, chemical industry, food and other fields. With the continuous development and improvement of probiotic microencapsulation technology, probiotic microcapsules have excellent acid resistance, bile salt resistance, storage resistance and enteric dissolution. Although the probiotics are fully protected, new problems arise one after another: after the probiotics reach the intestinal tract, their proliferation is inhibited due to factors such as nutrient deficiency and bile salt erosion, resulting in a certain amount of probiotics entering the human body. , but cannot proliferate in the intestinal tract, will not produce new bacteriocins, and the probability of colonization on the intestinal mucosa is also reduced. The probiotics ...
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