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Novel microorganism strain GM-020 of Lactobacillus rhamnosus and its use for treating obesity

a technology of lactobacillus rhamnosus and microorganisms, applied in the field of new lactobacillus rhamnosus microorganism strain gm020, can solve problems such as significant impairment of health

Inactive Publication Date: 2005-08-25
GENMONT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obesity is an excess of body fat usually due to changes of physiological or biochemical function and is a significant impairment of health.

Method used

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  • Novel microorganism strain GM-020 of Lactobacillus rhamnosus and its use for treating obesity
  • Novel microorganism strain GM-020 of Lactobacillus rhamnosus and its use for treating obesity
  • Novel microorganism strain GM-020 of Lactobacillus rhamnosus and its use for treating obesity

Examples

Experimental program
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Effect test

example 1

Isolation of Lactobacillus rhamnosus GM-020

[0058] A piece of human stomach tissue taken by an endoscope was cultured in 2 mL of Lactobacillus MRS Broth (DIFCO® 0881). The broth containing the tissue was plated on Lactobacillus selective agar and incubated at 37° C. for one day. Single colony growing on the plate was selected and subjected to Gram-stain. Gram-positive bacteria were then selected. One strain, called as Lactobacillus rhamnosus GM-020, was cloned.

example 2

Cell Wall Proteins Extraction and Analysis of GM-020

[0059] Twenty-four-hour-old cells of mesophilic lactobacilli cultivated in MRS broth (Difo®)) were harvested, washed twice in 0.05 M Tris-HCl (pH 7.5) containing 0.1 M CaCl2, and resuspended in 1 ml of the same buffer at an A600 of 10.0. After centrifugation at 8,000×g for 5 min, cell wall proteins were extracted from the pellets with 1.0 ml of extraction buffer (pH 8.0) containing 0.01 M EDTA, 0.01 M NaCl, and 2% (wt / vol) SDS. Suspensions were stored at room temperature for 60 min, heated at 100° C. for 5 min, and centrifuged at 11,600×g for 10 min at 4° C. The supernatants were analyzed by 12% SDS-PAGE and stained with Comassie blue.

example 3

Two-D Total Proteins Electrophoresis of GM-020

[0060] Ten mg of GM-020 cells were added with 0.5 mL of lysis buffer C (7M urea, 4% CHAPS, 2 M thiourea, 40 mM tris base, 0.5% IPG buffer amd 5 mM TBP) and 100 μL of glass beads. The solution was then subjected to sonicate and centrifuged at 10,000 rpm for 30 min. Total proteins were determined by Bio-rad PlusOne™ protein assay and then subjected to 2-D electrophoresis with pH 3 to 10 IEF. The electrophresis as programmed in Table 5 was performed with 10% gel and with 40 mA / gel for 4 hours. The gel was then fixed with 10% methanol and 7% acetic acid for 30 min. After fixation, the gel was colorized by sypro ruby stain for 5 hours and then destained with 10% methanl and 7% acetic acid for 6 hours. The result was shown in FIG. 3.

TABLE 5 30 V  12 hr 100 V0:10 hr 250 V0:10 hr 500 V0:10 hr1000 V0:30 hr4000 V0:30 hr6000 V  60 KVhr

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Abstract

The present invention provides an isolated microorganism strain, Lactobacillus rhamnosus GM-020, which is found to be effective in treating obesity and a complication thereof. The use of the Lactobacillus rhamnosus GM-020 in treating obesity and a complication thereof is also provided.

Description

BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] The invention mainly relates to a novel microorganism strain Lactobacillus rhamnosus GM-020 and its use for treating obesity or complications thereof. [0003] 2. Description of the Related Art [0004] Obesity is an excess of body fat usually due to changes of physiological or biochemical function and is a significant impairment of health. Fat usually contains neutral lipids, phospholipids and cholesterols. Weight gain is dependent on a person's energy intake being greater than energy expenditure. There are two types of obesity: (a) simple obesity, and (b) second obesity. Simple obesity is divided into idiopathic obesity and acquired obesity accounts for more 95% of obesity. Idiopathic obesity results from a large number of fat cell, and is usually found in childhood obesity. Acquired obesity results from a larger size of fat cell, and is usually found in adult obesity. Secondary obesity is known as symptomatic obesity...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K35/747A61K45/00C12N1/20C12R1/225
CPCA61K35/747A61K36/07C12N1/20C12N1/205C12R2001/225A61K2300/00
Inventor HSU, CHING-HSIANGSU, WEI-CHIH
Owner GENMONT BIOTECH
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