Lactobacillus rhamnosus capable of relieving chronic alcohol liver injury and application thereof

A kind of Lactobacillus rhamnosus, the technology of use, applied in the field of microbiology

Active Publication Date: 2012-08-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents do not fully relate to a probiotic Lactobacillus that can regulate intestinal flora and alleviate chronic alcoholic liver injury

Method used

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  • Lactobacillus rhamnosus capable of relieving chronic alcohol liver injury and application thereof
  • Lactobacillus rhamnosus capable of relieving chronic alcohol liver injury and application thereof
  • Lactobacillus rhamnosus capable of relieving chronic alcohol liver injury and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1: 16S rDNA sequence identification of Lactobacillus rhamnosus CCFM1107 of the present invention

[0084] Lactobacillus rhamnosus CCFM1107 was inoculated in MRS liquid medium, and cultured at 37°C for 18 hours. Take 1 mL of the culture liquid and use the bacterial genome DNA extraction kit to operate according to its instructions. Genomic DNA was used as a template, and bacterial identification universal primers published in literature (Critical Evaluation of Two Primers Commonly Used for Amplification of Bacterial 16S rRNA Genes. Applied and Environmental Microbiology, 2008, 74(8): 2461-2470) were used as primers, and 50 μL The reaction system is amplified by PCR, and then the amplified product is purified, recovered and sequenced.

[0085] The sequencing of the target gene PCR amplification product was completed by Shanghai Bioengineering Technology Service Co., Ltd. The sequencing results of the 16S rDNA of Lactobacillus rhamnosus CCFM1107 were compared us...

Embodiment 2

[0086] Example 2: Determination of microbiological properties of Lactobacillus rhamnosus CCFM1107

[0087] Lactobacillus rhamnosus CCFM1107 was inserted into the MRS liquid medium according to the inoculation amount of 5% based on the volume of the MRS liquid medium. At these time points of 18h, 20h, 22h and 24h, the pH value of the culture solution was measured with a pH meter, and the OD at 600nm was measured with a spectrophotometer 600 value. The pH meter that the present invention uses is the 320-S pH meter that Mettler-Toledo Instrument (Shanghai) Co., Ltd. sells, and the spectrophotometer that uses is the UV-2100 ultraviolet-visible spectrometer that Unico (Shanghai) Instrument Co., Ltd. sells Photometer.

[0088] by OD 600 Value and pH value are plotted against culture time, and the growth curve of Lactobacillus rhamnosus CCFM1107 in MRS medium can be obtained, and the results are as follows image 3 shown. In the MRS medium, the lag phase of Lactobacillus rhamnos...

Embodiment 3

[0089] Example 3, the antioxidant capacity of Lactobacillus rhamnosus CCFM1107

[0090] First, whole cells and cell-free extracts of Lactobacillus rhamnosus CCFM1107 were prepared.

[0091] Inoculate Lactobacillus rhamnosus CCFM1107 of the present invention into MRS liquid medium after activated culture, culture at 37°C for 24h, centrifuge at 6000r / min and 4°C for 10min to obtain culture supernatant and cell precipitation, the cell pellet was washed twice with sterile normal saline, resuspended in sterile normal saline, and the cell concentration was adjusted to about 10 9 cfu / mL. The obtained bacterial cell suspension was divided into two groups, one group was the complete cell group (IC), and the other group was used for the preparation of the cell-free extract (CFE). The cell suspension was ultrasonically crushed with an ultrasonic breaker (Sonics & Materials Company, VCX500 type) at 200W and a temperature of 4°C. Each treatment was carried out for 5s, and the interval be...

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Abstract

The invention relates to lactobacillus rhamnosus CCFM1107 capable of resisting oxidation and relieving chronic alcohol liver injury and also relates to application of the lactobacillus rhamnosus CCFM1107 to preparation of dairy products by using a working fermentation agent of the lactobacillus rhamnosus CCFM1107. The dairy product is milk, milk power, milk capsule products or fermented milk containing the lactobacillus rhamnosus CCFM1107. The lactobacillus rhamnosus CCFM1107 disclosed by the invention has high oxidation resistance, the capability of removing diphenyl picrylhydrazyl (DPPH) free radical and hydroxyl free radical, the capability of inhibiting lipid peroxidation and the capabilities of tolerating cholate, sodium chloride and pH. According to the lactobacillus rhamnosus CCFM1107 disclosed by the invention, the liver functions of a mouse suffered from the chronic alcohol liver injury and the antioxidant indexes can be improved; the serum endotoxin level is reduced; the distribution of intestinal flora is adjusted; and the chronic alcohol liver injury can be effectively relieved.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to a Lactobacillus rhamnosus capable of alleviating chronic alcoholic liver injury, and the present invention also relates to the use of the Lactobacillus rhamnosus. 【Background technique】 [0002] Alcohol abuse and alcohol dependence have become increasingly serious public health problems in the world today. In my country, alcohol-induced liver damage is on the rise year by year. Alcohol has become the second leading cause of liver damage after viral hepatitis. Alcohol damage to the liver mainly includes alcoholic fatty liver, alcoholic hepatitis, alcoholic liver fibrosis and alcoholic liver cirrhosis. Alcoholic liver damage can also lead to other hazards, such as causing the liver to fail to completely filter blood, causing hyperlipidemia, cardiovascular and cerebrovascular diseases; reducing liver catabolism, concurrent diabetes, chole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23C9/18A23C9/127C12R1/225A61K35/747
CPCA23Y2220/73A61K35/747A23C9/1234A61K35/74C12R1/225C12N1/20A23C9/18C12R2001/225C12N1/205A23V2400/175A23V2400/123A23V2400/249A23C9/1232A61K9/48A61K47/46A61K2035/11
Inventor 陈卫田丰伟黄文利赵建新张灏王刚张秋香刘小鸣范大明迟菲菲
Owner JIANGNAN UNIV
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