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Compound probiotics as well as production process and application thereof to leavening agent

A technology of compound probiotics and production process, which is applied to multi-purpose probiotic starters. The application field of this composite probiotics on starters can solve the problems of expensive equipment, high cost, and high process requirements, so as to improve product quality and production Efficiency, solving safety problems, improving the effect of source safety

Inactive Publication Date: 2014-11-19
SINO BIOTECH SHANGHAI CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the fermentation process of probiotics is relatively complicated, and the strains used in fermentation are relatively single, and there are relatively large limitations in application. The existing market products and technologies mainly have the following deficiencies:
[0008] 1) The single product contains few types of probiotics, and no enzyme preparation is added;
[0009] 2) Most of them are liquid, which is very inconvenient for transportation and storage, and at the same time, it is easy to lose activity and cause fermentation failure;
[0010] 3) Some with single function are only used for kimchi fermentation, while some are only used for feed fermentation;
[0011] 4) The fermentation process is relatively complicated and requires high technology;
[0012] 5) The energy consumption of the existing freeze-drying process is high, resulting in high cost, and the activity of the spray-dried bacteria is low, and the yield is low;
[0013] 6) The safety is poor, especially in feed fermentation, spores and yeasts are mostly used, and the starters using lactic acid bacteria are less, and the safety of spores and yeasts is not as good as lactic acid bacteria, and the colonization performance in animals is poor, and the probiotic effect is poor
The product yield of freeze-drying process is high, only need ultra-low temperature under vacuum, the equipment is expensive, and the energy consumption is very high at the same time, resulting in high production cost of bacterial powder
Spray drying has the advantages of simple process and low equipment investment, but because spray drying is carried out at a relatively high temperature, and the highest temperature resistance of probiotics is only 50°C, the activity of products obtained by spray drying is extremely low, and most of them are dead bacteria

Method used

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  • Compound probiotics as well as production process and application thereof to leavening agent

Examples

Experimental program
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Effect test

example 1

[0075] Example 1: Production of compound probiotic powder

[0076] 1) activated bacterial classification: the bacterial classification that the present invention adopts is:

[0077] Lactobacillus plantarum, deposit number: CGMCC1.557

[0078] Lactobacillus fermentum, deposit number: CGMCC1.2133

[0079] Lactobacillus casei, deposit number: CICC20983

[0080] Lactobacillus rhamnosus LactobaciUus rhamnosus, preservation number: CICC20300

[0081] The above strains are purchased raw materials, and the ratio of the strains contained in them is: 6:2:1:1;

[0082] Activated strains were prepared as follows:

[0083] Insert the frozen-preserved bacteria into the sterilized MRS medium, and incubate at a constant temperature of 37°C for 24 hours. After confirming that the colonies are free of bacteria, repeat the operation three times to obtain the activated bacteria.

[0084] The MRS medium used above is: casein peptone 10.0, beef extract 10.0, yeast extract 5.0, glucose 5.0, sod...

Embodiment 2

[0112] Embodiment two: fermented pickles

[0113] Ingredients: radish, cowpea, salt, sucrose, spices.

[0114] 1. Wash 1 kg of radish and cut into pieces.

[0115] 2. Prepare 4% salt water in cold boiled water, add 1% sucrose, and stir to fully dissolve.

[0116] 3. Add 1 gram of probiotic starter into the above salt water and fully dissolve;

[0117] 4. Put the radish into the marinating container and add the seasoning together;

[0118] 5. Inject the brine of the prepared bacteria into the pickling container, and completely immerse the radish in the brine;

[0119] 6. Seal and ferment for 3 days at 30 degrees to mature.

[0120] The kimchi prepared by the process has strong flavor, crispy taste and rich nutrition. The final pH value is 3.8, and the smoked nitrate content is all below 0.5 mg / kg after testing, meeting the food safety requirements.

[0121] The above-mentioned radish can also be replaced by other vegetables, such as cabbage, cabbage, etc.

Embodiment 3

[0122] Embodiment 3: Fermentation Northeast sauerkraut

[0123] 1. Arrange the harvested vegetables to remove rotten leaves, wash them, cut them in half or quarters, and put them neatly into the fermentation tank;

[0124] 2. Prepare 4% brine and stir to fully dissolve;

[0125] 3. Add the probiotic starter into the above-mentioned brine, fully dissolve, and put in the starter at a ratio of 0.2 to 1 kg / ton;

[0126] 4. Inject the prepared brine of the bacteria into the pickling container, and completely immerse the vegetables in the brine;

[0127] 5. Start the conveying pump and return the brine to fully mix the brine in the pickling pool;

[0128] 6. Cover the mouth of the fermentation tank and ferment at room temperature.

[0129] Northeast sauerkraut is fermented with the above-mentioned process. The fermentation time is 15 days in winter and 7-8 days in summer.

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Abstract

The invention provides compound probiotics as well as a production process and an application thereof to a leavening agent. The compound probiotics are obtained as follows: strains with strong tolerance and excellent fermentation performance are combined and proportioned to prepare the dry powder of probiotics by virtue of strain activation and cultivation, fermentation, separation and vacuum low-temperature drying; and then the dry powder of probiotics is compounded and packaged to obtain the complex probiotics. A continuous vacuum low-temperature drying process is adopted, the period is shortened and the yield is improved; and the complex probiotics comprises lactobacillus plantarum, lactobacillus fermentum, lactobacillus casei and lactobacillus rhamnosus in a ratio of (2-8) to (1-6) to (1-4) to (1-3); during application, every ton of vegetable can be well fermented within 3-5 days by use of 0.2-1kg of the leavening agent at a fermenting temperature of 20-30 DEG C, wherein the fermentation time is shortened by 50% in comparison with that in the conventional method, the yield of the salted and preserved vegetable is over 75%. The multipurpose probiotics leavening agent prepared by the production process disclosed by the invention can be used for the vegetable fermenting industry and the feed fermenting industry, so that a realistic problem in agricultural production and a food processing process is solved; and meanwhile, the product quality and the production efficiency are improved.

Description

technical field [0001] The present invention relates to a biological product, in particular to a multi-purpose probiotic starter. Specifically, the present invention also provides the production process of the composite probiotic. In particular, the present invention also provides the preparation of the composite probiotic on the starter. The application belongs to the field of biotechnology. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the intestinal tract and reproductive system of humans or animals and can produce definite health effects to improve the host's micro-ecological balance and exert beneficial effects. Among them, lactic acid bacteria are a relatively common type, which are widely used in the fields of bioengineering, breeding, health food and biomedicine. Probiotic microecological preparations have great development pote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20A23L1/218A23K1/00C12R1/25C12R1/245C12R1/225A23K10/12A23L19/20
Inventor 陈瑞张继学蔡桐
Owner SINO BIOTECH SHANGHAI CO
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