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Acidproof and bile-salt-resisting rhamnose lactobacillus strain with anti-enterovirus and antioxidant functions

A technology of Lactobacillus rhamnosus and microbial strains, applied in the field of microbiology, can solve problems such as failure to pass, lactic acid bacteria intolerant to gastric acid and bile, etc.

Active Publication Date: 2007-06-20
PRESIDENT ENTERPRISES (CHINA) INVESTMENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, although lactic acid bacteria strains have the ability to adhere to the small and large intestines, when they are ingested as food, many lactic acid bacteria cannot tolerate gastric acid and bile, and thus cannot pass through the stomach and reach the intestinal tract

Method used

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  • Acidproof and bile-salt-resisting rhamnose lactobacillus strain with anti-enterovirus and antioxidant functions
  • Acidproof and bile-salt-resisting rhamnose lactobacillus strain with anti-enterovirus and antioxidant functions
  • Acidproof and bile-salt-resisting rhamnose lactobacillus strain with anti-enterovirus and antioxidant functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Source and Collection of Samples

[0063] The bacterial strain of the present invention comes from two longevity villages in China, which are also the 4th and 5th longevity villages in the world - Bama Yao Autonomous County of Guangxi and feces of long-lived old people in Hotan area of ​​Xinjiang.

[0064] Bama County is located in the northwest of Guangxi and belongs to the mountainous and hilly area. The average altitude is 400-600 meters, there are many negative oxygen ions, and the air is fresh. Most of the locals live in mountains, river valleys, and forest areas without noise. Bama is located in the subtropical zone, where plants grow luxuriantly and are evergreen all year round, which is good for protecting eyesight. There are more than 2,000 species of plants, and abundant precipitation and water resources. Drinking water is a considerable degree of hard water, containing a large number of various trace elements. The rain and heat are in the same ...

Embodiment 2

[0068] Embodiment 2: Isolate lactic acid bacteria from feces of long-lived old people

[0069] The bacterial strain screening was carried out for 5 years (2000-2005). 54 feces samples from long-lived elderly (over 90 years old) in Bama, Guangxi and Hotan, Xinjiang were immersed in MRS broth culture and cultured anaerobically at 37°C for 24 hours. This culture was spread on an MRS agar plate, and then cultured at 37°C for 3 days. After cultivation, the colonies that appeared on the agar medium were collected and further purified. More than 1,000 strains of acid-producing bacteria were obtained by colony morphology screening, and lactic acid bacteria were obtained by measuring their products. According to the growth characteristics and metabolic characteristics, specific cultures were selected. Base, get the advantage of lactic acid bacteria. According to its characteristics, one strain of lactic acid bacteria with excellent characteristics is obtained from the three dominant l...

Embodiment 3

[0072] Example 3: Gram staining of lactic acid bacteria strains

[0073] The characteristics of the strains were detected by Gram staining. The results are shown in Figure 1, the bacterial strain of the present invention is a Gram-positive bacterium with no spores, a rod shape, and a size of 1.0-1.5 μm, which is a typical feature of the genus Lactobacillus.

[0074] Then, the lactic acid bacteria were studied to elucidate their culture characteristics. As a result, the strain grew into gray colonies with a diameter of 2-3mm on the MRS agar plate, and the optimum culture temperature was 37°C.

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Abstract

Lactobacillus rhamnosus and the use for it and its metabolin are disclosed. The strain is sieved and obtained from excrement of long-lived old. It's safe, has excellent antibacterial, acid-resisting and antioxidant performances. It can be used in dairy process and functional food production.

Description

technical field [0001] The invention belongs to the field of microbiology, and more specifically, the invention relates to a novel strain of Lactobacillus rhamnosus with acid resistance, bile salt resistance, intestinal pathogenic bacteria resistance and oxidation resistance. Background technique [0002] Lactic acid bacteria is a general term for bacteria that can ferment carbohydrates and mainly produce lactic acid. At present, a large number of researches on lactic acid bacteria have been carried out internationally, and lactic acid bacteria have been applied in many fields in practice. [0003] In general, lactic acid bacteria have an affinity for epithelial cells of digestive organs that contain a large amount of lectins. Thus, lactic acid bacteria obtained from the external environment tend to bind to this area in vivo and proliferate thereon. And, lactic acid bacteria that are daily present in the body form colonies on this accessory surface. [0004] The presence ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P1/00A23L1/29C12R1/225A23L33/00A61K35/747
Inventor 陈顺利顾瑞霞李正华席文博沈琴
Owner PRESIDENT ENTERPRISES (CHINA) INVESTMENT CO LTD
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