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Bacillus licheniformis and traditional Chinese medicine polysaccharide composition and its preparation

A technology of Bacillus licheniformis and a composition, applied in the field of microorganisms, can solve problems such as unfavorable improvement of curative effect, lack of clear influence of traditional Chinese medicine components, etc., and achieve the effects of improving intestinal colonization resistance, promoting significant proliferation and fast onset of effect.

Active Publication Date: 2017-06-13
SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above clinical literature, Zhengchangsheng is usually combined with a traditional Chinese medicine mixture that can invigorate the middle and replenish qi, dispel dampness and invigorate the spleen, but this application only combines the effects of Zhengchangsheng to promote intestinal microecological balance and the spleen-strengthening benefits of traditional Chinese medicine prescriptions. Qi functions are integrated together, and there is no clear effect of traditional Chinese medicine components on the regulation of intestinal health and micro-ecosystem
Moreover, the traditional Chinese medicine prescription is a mixture of multi-flavored medicinal materials. If there is no clear effect of each medicinal material in the prescription on the growth and reproduction of Zhengchangsheng or the metabolic transformation of Zhengchangsheng on the medicinal materials, it is not conducive to the combination of this prescription and Zhengchangsheng. Increased efficacy of medication

Method used

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  • Bacillus licheniformis and traditional Chinese medicine polysaccharide composition and its preparation
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  • Bacillus licheniformis and traditional Chinese medicine polysaccharide composition and its preparation

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Experimental program
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Effect test

Embodiment 1

[0013] It proves that effective additive doses of Lycium barbarum polysaccharides, Lentinan polysaccharides, Astragalus polysaccharides, Codonopsis polysaccharides or Acanthopanax polysaccharides can significantly promote the proliferation of Bacillus licheniformis CMCC63516, prolong the logarithmic growth phase of Bacillus licheniformis and shorten the logarithmic growth The doubling time of Bacillus licheniformis bacteria in the early stage can reduce the autolysis rate of bacteria.

[0014] Bacterial species: Bacillus licheniformis CMCC63516 (the strain number of China Medical Bacteria Collection Center)

[0015] Seed medium (g / 100ml): beef powder 0.5, peptone 1.0, NaCl 0.5, pH7.0-7.2.

[0016] Basal medium: same as seed medium.

[0017] Traditional Chinese medicine polysaccharide medium (g / 100ml): beef powder 0.5, peptone 1.0, NaCl 0.5, pH7.0-7.2, add different concentrations of Chinese medicine polysaccharide (0.01, 0.05, 0.10, 0.50, 1.00, 5.00).

[0018] method 【1】 : ...

Embodiment 2

[0029] It is proved that the effective dose of traditional Chinese medicine polysaccharides has different effects on promoting the proliferation of Bacillus licheniformis CMCC63516 under aerobic and anaerobic conditions.

[0030] The preparation of strains, seed medium, basal medium and traditional Chinese medicine polysaccharide medium are all the same as in Example 1.

[0031] method:

[0032] 1, seed preparation method and detection method are all with embodiment one.

[0033] 2. Detect the effect of polysaccharides of traditional Chinese medicine on the growth of Bacillus licheniformis. Culture in anaerobic environment, the gas composition is N 2 85%, H 2 10%, CO 2 5%, the culture temperature and ventilation are the same as in Example 1.

[0034] Table 2 The test results of the ability of traditional Chinese medicine polysaccharides to promote proliferation of CMCC63516 under anaerobic conditions

[0035] (Unit of live bacteria: 10 8 CFU / ml)

[0036]

[0037] It ...

Embodiment 3

[0039] shake tube test [2,3] To confirm the effect of different traditional Chinese medicine polysaccharides on promoting the ability of Bacillus licheniformis CMCC63516 to inhibit conditionally pathogenic Staphylococcus aureus under anaerobic conditions.

[0040] [2] Cheng Xiufang, Liu Hong, Zhang Zhiyan, etc., Research on the antibacterial properties of two strains of probiotics and their synbiotics on the intestinal flora of experimental mice [J] Qilu Pharmaceutical Affairs, 2010, 29(4): 234-236 .

[0041] [3] Wu Tielin, Ge Zhengxing. In vitro antibacterial test observation of antifungal vaccine against Candida albicans [J] Journal of Traditional Chinese Medicine, 2010, 25(146): 101-102.

[0042] Indicator bacteria: Staphylococcus aureus CMCC (B) 26003 was purchased from China Medical Bacteria Collection Center.

[0043] Staphylococcus aureus culture medium and culture conditions: 0.2% glucose, 1.0% peptone, 0.5% NaCl, 0.5% beef extract, pH 7.0, 37°C, 180r / min shaker cult...

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Abstract

A composition of Bacillus licheniformis and traditional Chinese medicine polysaccharides and its preparations widely used in the field of microbial technology, including Bacillus licheniformis, Chinese medicine polysaccharides, and auxiliary materials. The mass ratio of the bacteria powder to the Chinese herbal medicine extract is 1:1.25‑25, and the Chinese medicine polysaccharide is one of Lycium barbarum polysaccharide, Lentinan polysaccharide, Astragalus polysaccharide, Codonopsis polysaccharide and Acanthopanax polysaccharide; the polysaccharide content in the Chinese medicine polysaccharide is 70 %‑95%; under aerobic and anaerobic conditions, each traditional Chinese medicine polysaccharide has a proliferation effect on Bacillus licheniformis, and the multiplication effect of each traditional Chinese medicine polysaccharide on Bacillus licheniformis is related to the amount added in the seed medium; The composition is prepared as an oral preparation, and is prepared as a common tablet. The Bacillus licheniformis and traditional Chinese medicine polysaccharide composition of the invention can promote the significant proliferation of Bacillus licheniformis under both aerobic and anaerobic conditions, enhance its ability to restore the balance of intestinal flora, and rapidly improve the resistance of intestinal colonization. The composition is effective in treating diarrhea, etc. The onset of intestinal diseases is faster and the course of treatment is shorter.

Description

technical field [0001] The invention relates to a composition of bacillus licheniformis and traditional Chinese medicine polysaccharide and its preparation in the technical field of microorganisms. Background technique [0002] Chinese medicine extracts are microecological preparations that have emerged in recent years. After probiotics and traditional Chinese medicines are taken at the same time, a large number of supplemented probiotics can ensure that Chinese medicine extracts are quickly and effectively decomposed, absorbed and utilized. Chinese medicine extracts themselves directly promote normal bacteria. It can also be said that traditional Chinese medicine indirectly maintains the balance of the flora by promoting the growth of the normal flora, while probiotics directly maintain the balance of the flora by supplementing beneficial bacteria , both of them have the same effect and lead to the same goal by different routes, but Chinese medicine preparations are better ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/742A61P1/00A61P1/12A61K31/715
Inventor 雷健彭勃吴楠邢丹何新李雪龙刘心
Owner SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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