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Peach gum polyferose and application thereof

A peach gum polysaccharide and reaction technology, applied in peach gum polysaccharide iron and its application field, can solve the problems of high price of lactoferrin, etc., achieve the effects of preventing respiratory tract infection and diarrhea, improving the immune system, and high iron content

Inactive Publication Date: 2019-08-27
深圳保时健生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, relying solely on food supplements cannot meet the needs of iron-deficient infants. The digestive system of infants is not yet perfect, and the requirements for the safety and effectiveness of iron supplements are higher. At present, the common iron supplements for infants are: gluconate Iron, ferrous lactate, lactoferrin, etc.
Among them, lactoferrin is an important non-heme iron-binding glycoprotein in breast milk. Lactoferrin in human colostrum can reach 6-14 mg / ml, while that in normal breast milk is only 1-3.2 mg / ml. 0.02—0.35mg / ml, so the price of real lactoferrin is high

Method used

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  • Peach gum polyferose and application thereof
  • Peach gum polyferose and application thereof
  • Peach gum polyferose and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of Peach Gum Polysaccharide Extract:

[0035] S11: Wash 50g of raw peach gum to remove impurities, add 1000ml of water to soak for 8h, heat the homogenate to 100°C for 1h after crushing, centrifuge at 2000rpm / min for 3min, take the supernatant to obtain a crude sugar solution;

[0036]S12: Add absolute ethanol to the crude sugar solution described in step S11 at a ratio of 1:1, fully shake it and let it stand at 4°C for 12 hours, centrifuge at 4000rpm / min, take the precipitate and repeat step S12 twice, then freeze-dry the precipitate, Pulverize to obtain the peach gum polysaccharide crude extract as 42g;

[0037] S13: Purification, the peach gum polysaccharide crude extract in step S12 is added with water to form a 2mol / mL polysaccharide solution, the fiber column is pretreated, and then deionized water is used to equilibrate the DEAE-52 cellulose column, and the polysaccharide solution is slowly passed through the sample, Finally, it was eluted with 0.2mo...

Embodiment 2

[0044] Preparation of Peach Gum Polysaccharide Extract:

[0045] S11: Wash 50g of raw peach gum to remove impurities, add 500ml of water to soak for 10h, heat the homogenate to 100°C for 2h after crushing, centrifuge at 4000rpm / min for 3min, take the supernatant to obtain a crude sugar solution;

[0046] S12: Add absolute ethanol to the crude sugar solution described in step S11 at twice the amount of the crude sugar solution, shake it fully and let it stand at 0°C for 12 hours, centrifuge at 4000rpm / min, take the precipitate and repeat step S12 for 3 times, then freeze the precipitate Dried and pulverized to obtain the crude extract of peach gum polysaccharide;

[0047] S13: Add water to the peach gum polysaccharide crude extract in step S12 to form a 2.5mol / mL polysaccharide solution, pass through a DEAE-52 cellulose column, elute with 0.5mol / mL NaCl solution, collect the eluted samples, and Filter through a μm microporous membrane, concentrate and dry to obtain 30 g of pea...

Embodiment 3

[0054] Preparation of Peach Gum Polysaccharide Extract:

[0055] S11: Wash 50g of raw peach gum to remove impurities, add 800ml of water to soak for 10h, heat the homogenate to 100°C for 2h after crushing, centrifuge at 4000rpm / min for 3min, take the supernatant to obtain a crude sugar solution;

[0056] S12: Add 5 times the volume of absolute ethanol to the crude sugar solution described in step S11, shake it fully, let it stand at 4°C for 12 hours, centrifuge at 6000rpm / min, take the precipitate and repeat step S12 for 3 times, freeze-dry the precipitate, Pulverize to get peach gum polysaccharide crude extract;

[0057] S13: Add water to the crude peach gum polysaccharide extract in step S12 to form a 5mol / mL polysaccharide solution, pass through a DEAE-52 cellulose column, elute with a 1mol / mL NaCl solution, collect the eluted sample, and filter it in a dialysis bag , concentrated and dried to obtain 37g of peach gum polysaccharide extract.

[0058] Preparation of peach g...

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Abstract

The invention provides peach gum polyferose which is a peach gum polyferose compound having an iron core center similar to iron proteinate and formed by chelating a peach gum polysaccharide with iron.The peach gum polyferose provided by the invention has beneficial effects that the peach gum polyferose has high iron content and complexation stability; no free Fe<2+> exists in the product; no irritation is caused to the gastrointestinal tract; the iron supplementing effect is good; no toxic substances are used in the production process, and the product has good safety. The adding amount is low. After the iron in the peach gum polyferose is released, the peach gum polysaccharide is a supplement of probiotic nutrition, thereby promoting the biological activity of the probiotic bacteria in intestinal colonization; moreover, the peach gum polysaccharide has a natural function of adsorbing harmful substances, and is also a very good water-soluble dietary fiber, which has good water retention property, can increase the moisture and looseness of feces, and is help to improve the intestinal functions.

Description

technical field [0001] The invention belongs to the technical field of food and medicine, and more specifically relates to iron peach gum polysaccharide and its application. Background technique [0002] Iron is an essential trace element for the human body. If the human body does not have enough iron intake, the synthesis of hemoglobin will be reduced, and the content of hemoglobin in red blood cells will be reduced, resulting in a decrease in the number of red blood cells. As a result, various tissues and organs in the human body will insufficient oxygen supply. Iron deficiency anemia is one of the nutrient deficiency diseases with the highest incidence rate, which seriously threatens human health. According to the 2013 Global Burden of Disease (GBD) study, anemia is the top five diseases that impair healthy lifespan. Anemia damages the functions of human organs and tissues, hinders the growth and development of people at all stages, and easily causes functional developm...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K33/26A61K35/745A61K35/747A61P3/02A61P7/06A61P37/04
CPCA61K33/26A61K35/745A61K35/747A61P3/02A61P7/06A61P37/04C08B37/0003C08B37/006A61K2300/00
Inventor 占晖
Owner 深圳保时健生物工程有限公司
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