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Bacillus and application thereof

The technology of Bacillus licheniformis and strains, applied in the field of microorganisms, can solve the problems of lowering the application level of animal feed, lowering enzyme activity, long fermentation cycle, etc., and achieves the effects of being conducive to industrialized popularization and use, broad application prospects and short fermentation cycle

Active Publication Date: 2018-11-23
山东宝来利来生物工程股份有限公司
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The industrial production of animal feed microecological preparations often adopts the method of heat treatment and granulation of fermented bacteria liquid. However, the heat resistance of the current Bacillus is poor, and the number of viable bacteria and the enzyme activity of the secreted alkaline phosphatase will be reduced during heat treatment, thereby The effective number of viable bacteria and enzyme activity of probiotics products cannot be guaranteed, thus reducing their application level in animal feed
[0006] In addition, the current fermentation period of the existing Bacillus is relatively long, and the growth and reproduction of the early strains occupy a part of the fermentation period, and the production of alkaline phosphatase is relatively low
[0007] To sum up, it can be seen that the production technology of alkaline phosphatase, colonization performance and high-efficiency fermentation of Bacillus currently need to be studied and solved.

Method used

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  • Bacillus and application thereof
  • Bacillus and application thereof
  • Bacillus and application thereof

Examples

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preparation example Construction

[0046] In a typical embodiment of the present invention, a kind of preparation method of probiotics with high production alkaline phosphatase and good colonization performance is provided, comprising:

[0047] A preparation method of a microecological agent with high yield of alkaline phosphatase and good colonization performance, comprising:

[0048] (1) Fermentation of strains:

[0049] Strain activation: inoculate strain BLCC1-0441 with fresh LB slant, culture at 35-40°C for 18-30 hours, and store the slant for future use after the spore rate is over 90% under the microscope.

[0050] Preparation of seed solution: prepare seed solution with LB liquid medium, the liquid volume is 18-22 (v / v)%; inoculate two loops from the slope into fresh medium, and cultivate at 35-40°C, 150-200r / min , Carry out fermentation tank inoculation when microscopic examination spore rate is more than 90%.

[0051] Fermentation culture: The formula of the fermentation medium is, by mass percentag...

Embodiment

[0059] In this project, the spore strains with strong alkaline phosphatase production were screened by the method of disodium benzene phosphate using microporous plates, the properties of the alkaline phosphatase of the strains were studied, and the fermentation conditions were optimized to improve the enzyme-producing activity of the strains.

[0060] 1 strain screening

[0061] 1.1 Strains to be screened

[0062] The strains to be screened were all obtained from the strain preservation center of Bioengineering Research Institute of Shandong Baolai Lilai Bioengineering Co., Ltd., and were isolated and screened from livestock and poultry breeding manure in Taishan District, Tai'an City.

[0063] 1.2 Reagents

[0064] (1) 0.1mol / L carbonate buffer (pH10.0): Weigh 0.64g of anhydrous sodium carbonate, 0.34g of sodium bicarbonate, and 0.15g of 4-aminoantipyrine, and dissolve them in 100mL of distilled water.

[0065] (2) 0.02 mol / L disodium phenyl phosphate solution: weigh 0.51 ...

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Abstract

The invention discloses a bacillus. The strain is classified and named as bacillus licheniformis, is numbered as BLCC1-0441 and has been preserved at China Center for Type Culture Collection (CCTCC) on May 7, 2018; the address is Wuhan University, Wuhan, China; its preservation number is CCICC NO.: 2018253. The bacillus is capable of highly yielding alkaline phosphatase as well as efficiently fermenting, has excellent intestinal colonization performance and has an excellent application in chicken breeding.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus strain and its application. Background technique [0002] At present, it has been clarified that harmful bacteria can induce adverse immune responses and lead to infectious diseases through the interaction of endotoxin lipopolysaccharides released by them with Toll-like receptors on the intestinal epithelial cell membrane of host animals. Endotoxins (LPS) are cell wall lipopolysaccharide components released by Gram-negative bacteria during growth or cleaved upon death. The basic structure of LPS is composed of three parts connected by covalent bonds, namely O-specific antigen polysaccharide, core polysaccharide and lipid A. Lipid A has been confirmed as the active molecule of endotoxin, which can mediate almost all the biological characteristics of endotoxin, and the double phosphorus bond of lipid A is the main group of its biological activity. Al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/10
CPCA23K10/18C12N1/20C12N1/205C12R2001/10
Inventor 陈雷单宝龙王春凤谷巍徐海燕汪祥燕姜延龙辛国芹董佩佩刘元香王红李金敏陈振赵影张高娜
Owner 山东宝来利来生物工程股份有限公司
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