Bifidobacterium for selenium-enriched culture and its active protein and application thereof

Abstract

The present invention discloses a novel Bifidobacterium DD98 having a 16SrDNA nucleotide sequence shown in SEQ ID No: 1. Further, the Bifidobacterium is preserved in the General Microbial Culture Collection Management Center of China, and a preservation number is CGMCC No. 16573. The Bifidobacterium DD98 of the invention can tolerate higher selenium pressure, can be used for selenium-enriched culture, and has good intestinal colonization ability, and the extracted water-soluble protein has intestinal protection function. The Bifidobacterium DD98 of the invention can alleviate liver damage caused by a high-fat diet and alcohol, and has the protective effect on the liver.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a bifidobacterium and its active protein and its application. Further, the bifidobacteria can be prepared into selenium-enriched probiotics through selenium-enriched culture. Background technique [0002] Probiotics are a class of microorganisms that can have beneficial effects on the host and are closely related to human health. Probiotics generally colonize the intestinal tract and reproductive system of the human body, and play an important role in maintaining the micro-ecological balance in the human body. Existing research results have shown that the stability of human microbiota is very important for maintaining human health. Aging, hypertension, diabetes and intestinal diseases are all related to human microbiota. The imbalance of microbiota in the human body will directly or indirectly lead to the occurrence of these diseases. Bifidobacterium is a common ...

AI Technical Summary

Problems solved by technology

However, there are very few Bifidobacterium strains available in food at home and abroad. This is because after Bifidobacteria are orally ingested into the human body, various factors will affect their colonization in the human intestinal tract, for example, the low pH value of the stomach The high acidic environment and the digestive enzymes in it, the acidic environment will reduce the proton power and destroy the osmotic pressure balance inside and outside the bifidobacteria cells; the high concentration of bile salts and endogenous antimicrobial peptides in the small intestine and the intestinal bacteria The group's defense system to the body, glycocholate and bezoar bile salt have cell membrane toxicity, bifidobacteria with high activity of bile salt hydrolyzing enzyme can decompose bile salt into secondary bile salt, which can reduce its cell membrane toxicity; Bifidobacterium adhesion The ability to enter the intestinal epithelial cells and the ability to utilize nutrients that are not digested and metabolized by the host (some protein substances, prebiotics such as oligosaccharides, etc.) will affect the intestinal colonization of bifidobacteria

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