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Bifidobacterium lactis JYBR-390 with constipation treatment effect as well as application and product of bifidobacterium lactis JYBR-390

A technology of JYBR-390 and Bifidobacterium lactis, applied in the field of probiotics, can solve the problems of obvious, many adverse reactions, and little efficacy, and achieve the effect of improving constipation, avoiding negative effects, and improving the water content of feces

Active Publication Date: 2022-01-25
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the technical problems that intestinal prokinetic drug therapy and laxative drug therapy have many adverse reactions, traditional Chinese medicine therapy has little curative effect or is not suitable for long-term use, and diet and exercise therapy problems cannot be cured unilaterally, the present invention provides a strain that has the effect of treating constipation Bifidobacterium lactis JYBR-390 and its application and products. The present invention uses the characteristics of Bifidobacterium lactis JYBR-390, which has strong intestinal colonization ability and the ability to inhibit pathogenic bacteria, to prepare a product for treating constipation by clarifying the mechanism of constipation. , the laxative effect of the product is obvious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The preparation method of the PBS buffer used is: Weigh 0.27g of potassium dihydrogen phosphate, 1.42g of disodium hydrogen phosphate, 8g of sodium chloride and 0.2g of potassium chloride, add about 800mL of deionized water to fully stir to dissolve, add concentrated Adjust the pH of the solution to 7.4 with hydrochloric acid, then dilute to 1 L with deionized water, and sterilize at 121°C for 20 minutes.

[0029] The components of the DMEM complete culture solution used are: 78% DMEM culture solution, 20% fetal bovine serum, 1% streptomycin (100 μg / mL), and 1% penicillin (100 U / mL).

[0030] The preparation method of the ink used is: take 10g gum arabic, add a small amount of water to boil, then add 5g of activated carbon powder and boil three times, set the volume to 100mL after standing at room temperature, store at 4°C, and shake well before use.

Embodiment 1

[0031] Example 1 Isolation and identification of strains

[0032] 1. Strain screening and purification

[0033] (1) Sampling: In August 2014, 1 mL of naturally fermented yoghurt was taken from the herdsman's home in Yili Prefecture, Xinjiang, and set aside;

[0034] (2) Preparation of samples: ① Take sterilized physiological saline (0.85%) and place it in a sterile Erlenmeyer flask, then add the sample from step (1) into it, oscillate, and set aside;

[0035] ②Dilute the solution in step ① to make samples with different concentration gradients, each of which is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 , the labels are 1#, 2#, 3#, 4#, 5#, 6#, 7#, to be used;

[0036] (3) Cultivation: Spread 1#, 2#, 3#, 4#, 5#, 6#, and 7# solutions on the MRS plate medium with a spreader, and place them under anaerobic conditions at 37°C Cultivate for 48 hours;

[0037] (4) Select colonies: select colonies according to the characteristics of colonies with a diameter of 1-2 mm, s...

Embodiment 2

[0044] Embodiment 2 Preparation of products for treating constipation

[0045] (1) Activate the preserved Bifidobacterium lactis JYBR-390 on the MRS plate medium. After activation, inoculate 1% of the inoculum in the MRS liquid medium, and culture it anaerobically at 37°C for 48 hours to obtain bacteria liquid;

[0046] (2) After centrifuging the bacterial liquid, collect the bacterial cells, wash with sterile saline, and resuspend in 15wt% reconstituted skim milk to obtain a suspension;

[0047] (3) Adjust the concentration of the suspension to 1.0×10 10 cfu / mL, obtain the bacterial suspension, freeze-dry the bacterial suspension to obtain bacterial powder;

[0048] (4) Mix the bacterial powder and isomalto-oligosaccharide to make bacterial agents with bacterial counts of 100 million, 1 billion, 10 billion, and 15 billion cfu / g, respectively.

[0049] In other embodiments, when preparing the product for treating constipation, step (3) can adjust the suspension to 1.5×10 1...

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Abstract

The invention relates to bifidobacterium lactis JYBR-390 with a constipation treatment effect as well as an application and product of the bifidobacterium lactis JYBR-390. The bifidobacterium lactis JYBR-390 is preserved in China General Microbiological Culture Collection Center on July 8, 2019, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18093, and the bifidobacterium lactis JYBR-390 is classified and named as Bifidobacterium lactis; and the bifidobacterium lactis JYBR-390 is used for preparing a product for treating constipation. The bifidobacterium lactis JYBR-390 has the characteristics of strong intestinal colonization capability and pathogenic bacterium inhibition capability by determining a constipation action mechanism, so that the product for treating constipation is prepared, and the product has an obvious bowel relaxing effect.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a strain of Bifidobacterium lactis JYBR-390 with a therapeutic effect on constipation and its application and products. Background technique [0002] Constipation refers to the symptoms of decreased defecation frequency, difficulty in defecation, and dry stool. The etiology of constipation is usually related to bad living habits, intestinal diseases, and psychological disorders. At present, the treatment methods for constipation mainly include intestinal prokinetic drug therapy, laxative drug therapy, traditional Chinese medicine therapy, diet and exercise therapy, etc. [0003] Intestinal prokinetic drug therapy: At present, the main clinical intestinal prokinetic drugs are 5-HT4 receptor agonists, which can stimulate enteric plexus neurons, cause intestinal peristalsis, intestinal fluid secretion, shorten intestinal transit time, and reduce feces The recovery of internal wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10C12R1/01
CPCC12N1/20A61K35/745A61P1/10Y02A50/30
Inventor 张华磊马磊裴景广王丽媛司书锋曹维超金伟刘红亮赵广义孙川
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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