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A strain of Bifidobacterium lactis jybr-390 with therapeutic effect on constipation and its application and products

A technology of JYBR-390 and Bifidobacterium lactis, which is applied in the field of probiotics, can solve the problems of obvious, many adverse reactions, and little curative effect, and achieve the effect of improving constipation, avoiding negative effects, and improving the water content of feces

Active Publication Date: 2022-07-12
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the technical problems that intestinal prokinetic drug therapy and laxative drug therapy have many adverse reactions, traditional Chinese medicine therapy has little curative effect or is not suitable for long-term use, and diet and exercise therapy problems cannot be cured unilaterally, the present invention provides a strain that has the effect of treating constipation Bifidobacterium lactis JYBR-390 and its application and products. The present invention uses the characteristics of Bifidobacterium lactis JYBR-390, which has strong intestinal colonization ability and the ability to inhibit pathogenic bacteria, to prepare a product for treating constipation by clarifying the mechanism of constipation. , the laxative effect of the product is obvious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The preparation method of the used PBS buffer is as follows: weigh 0.27 g of potassium dihydrogen phosphate, 1.42 g of disodium hydrogen phosphate, 8 g of sodium chloride and 0.2 g of potassium chloride, add about 800 mL of deionized water, fully stir and dissolve, add concentration Adjust the pH of the solution to 7.4 with hydrochloric acid, then dilute to 1 L with deionized water, and sterilize at 121 °C for 20 min.

[0029] The components of the DMEM complete medium used were: 78% DMEM medium, 20% fetal bovine serum, 1% streptomycin (100 μg / mL), and 1% penicillin (100 U / mL).

[0030] The preparation method of the used ink is as follows: take 10 g of gum arabic, add a small amount of water to boil, then add 5 g of activated carbon powder to boil for three times, set the volume to 100 mL after placing at room temperature, store at 4°C, and shake well before use.

Embodiment 1

[0031] Example 1 Isolation and identification of bacterial species

[0032] 1. Strain screening and purification

[0033] (1) Sampling: In August 2014, 1 mL of naturally fermented yogurt was taken from herdsmen's homes in Ili Prefecture, Xinjiang, for use;

[0034] (2) Preparation of samples: ① Take sterilized physiological saline (0.85%) and put it in a sterile conical flask, then add the sample of step (1) into it, shake it, and set it aside for use;

[0035] ② Dilute the solution in step ① to prepare samples with different concentration gradients, 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 , the labels are 1#, 2#, 3#, 4#, 5#, 6#, 7#, and they are ready for use;

[0036] (3) Cultivation: Coat the 1#, 2#, 3#, 4#, 5#, 6#, and 7# solutions on the MRS plate medium using a spreader respectively, at 37°C under anaerobic conditions Culture for 48h;

[0037] (4) Select colonies: select colonies according to the characteristics of colonies with a diameter of 1-2m...

Embodiment 2

[0044] Example 2 Preparation of products for treating constipation

[0045] (1) The preserved Bifidobacterium lactis JYBR-390 was activated on the MRS plate medium, and then inoculated into the MRS liquid medium at an inoculum of 1% after activation, and cultured at 37 °C for 48 hours anaerobically to obtain bacteria. liquid;

[0046] (2) collecting the bacterial cells after centrifuging the bacterial liquid, washing with sterile physiological saline, and resuspending in 15wt% reconstituted skim milk to obtain a suspension;

[0047] (3) Adjust the concentration of the suspension to 1.0×10 10 cfu / mL to obtain bacterial suspension, freeze-dry the bacterial suspension to obtain bacterial powder;

[0048] (4) Mix the bacterial powder and isomaltose oligosaccharide to prepare bacterial preparations with the number of bacterial cells of 100 million, 1 billion, 10 billion, and 15 billion cfu / g.

[0049] In other embodiments, when preparing a product for treating constipation, step...

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PUM

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Abstract

The present invention relates to a strain of Bifidobacterium lactis JYBR-390 with a therapeutic effect on constipation, and applications and products thereof. Microbiology Center, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No. 18093, and the classification name is Bifidobacterium lactis Bifidobacterium lactis ; Described Bifidobacterium lactis JYBR-390 is used to prepare the product for the treatment of constipation. By clarifying the mechanism of action of constipation, the invention utilizes the characteristics that Bifidobacterium lactis JYBR-390 has strong intestinal colonization ability and pathogenic bacteria inhibition ability to prepare a product for treating constipation, and the product has an obvious laxative effect.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a strain of Bifidobacterium lactis JYBR-390 with constipation treatment effect and its application and products. Background technique [0002] Constipation is a condition in which the frequency of defecation decreases, at the same time, it is difficult to defecate, and the stool is dry. The cause of constipation is usually related to bad living habits, intestinal diseases, and psychological disorders. At present, the treatment methods of constipation mainly include intestinal motility drug treatment, laxative drug treatment, traditional Chinese medicine treatment and diet and exercise treatment. [0003] Intestinal prokinetic drug therapy: Currently, the main clinical prokinetic drugs are 5-HT4 receptor agonists, which can stimulate enteric plexus neurons, cause intestinal peristalsis, intestinal secretion, shorten intestinal transit time, and reduce feces. The recovery of in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10C12R1/01
CPCC12N1/20A61K35/745A61P1/10Y02A50/30
Inventor 张华磊马磊裴景广王丽媛司书锋曹维超金伟刘红亮赵广义孙川
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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