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Bacillus licheniformis/traditional Chinese medicine polysaccharide composition and preparations thereof

A technology of Bacillus licheniformis and a composition, applied in the field of microorganisms, can solve the problems of lack of clear influence of traditional Chinese medicine components, unfavorable improvement of curative effect, etc., and achieve the effects of improving intestinal colonization resistance, promoting significant proliferation, and shortening the course of treatment

Active Publication Date: 2014-09-03
SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above clinical literature, Zhengchangsheng is usually combined with a traditional Chinese medicine mixture that can invigorate the middle and replenish qi, dispel dampness and invigorate the spleen, but this application only combines the effects of Zhengchangsheng to promote intestinal microecological balance and the spleen-strengthening benefits of traditional Chinese medicine prescriptions. Qi functions are integrated together, and there is no clear effect of traditional Chinese medicine components on the regulation of intestinal health and micro-ecosystem
Moreover, the traditional Chinese medicine prescription is a mixture of multi-flavored medicinal materials. If there is no clear effect of each medicinal material in the prescription on the growth and reproduction of Zhengchangsheng or the metabolic transformation of Zhengchangsheng on the medicinal materials, it is not conducive to the combination of this prescription and Zhengchangsheng. Increased efficacy of medication

Method used

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  • Bacillus licheniformis/traditional Chinese medicine polysaccharide composition and preparations thereof
  • Bacillus licheniformis/traditional Chinese medicine polysaccharide composition and preparations thereof
  • Bacillus licheniformis/traditional Chinese medicine polysaccharide composition and preparations thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] It proves that effective additive doses of Lycium barbarum polysaccharides, Lentinan polysaccharides, Astragalus polysaccharides, Codonopsis polysaccharides or Acanthopanax polysaccharides can significantly promote the proliferation of Bacillus licheniformis CMCC63516, prolong the logarithmic growth phase of Bacillus licheniformis and shorten the logarithmic growth The doubling time of Bacillus licheniformis bacteria in the early stage can reduce the autolysis rate of bacteria.

[0014] Bacterial species: Bacillus licheniformis CMCC63516 (the strain number of China Medical Bacteria Collection Center)

[0015] Seed medium (g / 100ml): beef powder 0.5, peptone 1.0, NaCl 0.5, pH7.0-7.2.

[0016] Basal medium: same as seed medium.

[0017] Traditional Chinese medicine polysaccharide medium (g / 100ml): beef powder 0.5, peptone 1.0, NaCl 0.5, pH7.0-7.2, add different concentrations of Chinese medicine polysaccharide (0.01, 0.05, 0.10, 0.50, 1.00, 5.00).

[0018] method 【1】 : ...

Embodiment 2

[0029] It is proved that the effective dose of traditional Chinese medicine polysaccharides has different effects on promoting the proliferation of Bacillus licheniformis CMCC63516 under aerobic and anaerobic conditions.

[0030] The preparation of strains, seed medium, basal medium and traditional Chinese medicine polysaccharide medium are all the same as in Example 1.

[0031] method:

[0032] 1, seed preparation method and detection method are all with embodiment one.

[0033] 2. Detect the effect of polysaccharides of traditional Chinese medicine on the growth of Bacillus licheniformis. Culture in anaerobic environment, the gas composition is N 2 85%, H 2 10%, CO 2 5%, the culture temperature and ventilation are the same as in Example 1.

[0034] Table 2 The test results of the ability of traditional Chinese medicine polysaccharides to promote proliferation of CMCC63516 under anaerobic conditions

[0035] (Unit of live bacteria: 10 8 CFU / ml)

[0036]

[0037] It ...

Embodiment 3

[0039] shake tube test [2,3] To confirm the effect of different traditional Chinese medicine polysaccharides on promoting the ability of Bacillus licheniformis CMCC63516 to inhibit conditionally pathogenic Staphylococcus aureus under anaerobic conditions.

[0040] [2] Cheng Xiufang, Liu Hong, Zhang Zhiyan, etc., Research on the antibacterial properties of two strains of probiotics and their synbiotics on the intestinal flora of experimental mice [J] Qilu Pharmaceutical Affairs, 2010, 29(4): 234-236 .

[0041] [3] Wu Tielin, Ge Zhengxing. In vitro antibacterial test observation of antifungal vaccine against Candida albicans [J] Journal of Traditional Chinese Medicine, 2010, 25(146): 101-102.

[0042] Indicator bacteria: Staphylococcus aureus CMCC (B) 26003 was purchased from China Medical Bacteria Collection Center.

[0043] Staphylococcus aureus culture medium and culture conditions: 0.2% glucose, 1.0% peptone, 0.5% NaCl, 0.5% beef extract, pH 7.0, 37°C, 180r / min shaker cult...

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Abstract

A bacillus licheniformis / traditional Chinese medicine polysaccharide composition and preparations thereof, which are widely used in the technical field of microorganism, are disclosed. The composition comprises bacillus licheniformis, traditional Chinese medicine polysaccharides and auxiliary materials. The bacillus licheniformis relates to bacillus licheniformis CMCC63516 living bacteria powder. The weight ratio of the bacillus licheniformis CMCC63516 living bacteria powder to a traditional Chinese medicine extract product is 1:1.25-25. The traditional Chinese medicine polysaccharides are one of lycium barbarum polysaccharides, lentinan, astragalus polysaccharides, codonopsis pilosula polysaccharides, and acanthopanax senticosus polysaccharides. The polysaccharide content in the traditional Chinese medicine polysaccharides is 70-95%. In aerobic and anaerobic conditions, each traditional Chinese medicine polysaccharide has a multiplication effect for the bacillus licheniformis, and the multiplication effect of the traditional Chinese medicine polysaccharides is associated with the adding amount in a seed culture medium. The composition is prepared into an oral preparation and prepared into common tablets. The composition promotes significant multiplication of the bacillus licheniformis under aerobic and anaerobic conditions, enhances the capability of restoring intestinal flora balance, and rapidly enhances the intestinal colonization resistance. The composition is rapid to take effect and short in course of treatment when treating diarrhea and other intestine diseases.

Description

technical field [0001] The invention relates to a composition of bacillus licheniformis and traditional Chinese medicine polysaccharide and its preparation in the technical field of microorganisms. Background technique [0002] Chinese medicine extracts are microecological preparations that have emerged in recent years. After probiotics and traditional Chinese medicines are taken at the same time, a large number of supplemented probiotics can ensure that Chinese medicine extracts are quickly and effectively decomposed, absorbed and utilized. Chinese medicine extracts themselves directly promote normal bacteria. It can also be said that traditional Chinese medicine indirectly maintains the balance of the flora by promoting the growth of the normal flora, while probiotics directly maintain the balance of the flora by supplementing beneficial bacteria , both of them have the same effect and lead to the same goal by different routes, but Chinese medicine preparations are better ...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P1/00A61P1/12
Inventor 雷健彭勃吴楠李雪龙刘心
Owner SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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