Method for genetic modification of intestinal protobacteria based on microencapsulation treatment

A genetic modification and microencapsulation technology, applied in microorganism-based methods, transforming growth factors, chemical instruments and methods, etc., can solve the problems of not fully meeting research needs, high price of germ-free mice, and long transplantation period, etc. To achieve the effect of efficient and convenient colonization, improved biological compliance, and low cost
CN110051698AInactive Publication Date: 2019-07-26TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2019-07-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for genetic modification of intestinal protobacteria based on microencapsulation treatment. The method mainly includes utilizing the method of synthetic biology to genetically transform the selected intestinal primary escherichia coli and then perform microencapsulation treatment. The biosafety synthetic biology technology is combined with the non-invasive optogenetic technique for the treatment of intestinal diseases, and the method has many advantages compared with the traditional methods for treating the intestinal diseases. The intestinal primary escherichia coli are subjected to genetic modification, and the intestinal colonization compliance of the microencapsulated protobacteria is improved in the later stage. A single functional bioactive molecule,that is, a transforming growth factor (TGF-beta1) secreted by the primary escherichia coli is taken as an example to study the application in treatment of inflammatory bowel disease (IBD). The methodprovides a new idea for studying the interaction between enteric microorganisms and the body.
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Description

technical field

[0001] The invention relates to the field of microorganisms and clinical treatment, in particular to a method for genetic modification of intestinal protobacteria based on microencapsulation treatment. Background technique

[0002] As the largest organ in an organism, the intestine plays an important role in digestion and absorption, immune regulation and other aspects. In addition to epithelial cells and immune cells that perform digestion, absorption, and immune regulation functions, the intestinal tract also contains many microorganisms. More than 99.9% of the microorganisms are bacteria, which are collectively known as the gut flora.

[0003] Gut flora, as a "symbiont" in the gut of organisms, participates in the regulation of many important life processes and diseases. Most of the existing methods for studying the relationship between intestinal microbes and the body are to use germ-free mice or extensive flora transplantation, combined with microbiome...

Claims

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