Preparation method and application of aquatic fermented feed containing composite probiotic agent

Abstract

The invention relates to the technical field of aquaculture, and discloses a preparation method of an aquatic fermented feed containing a composite probiotic inoculant, and the preparation method comprises the following steps: mixing bacillus amyloliquefaciens, streptococcus lactis and saccharomycetes in proportion to obtain the composite probiotic inoculant; the preparation method comprises the following steps: dissolving a compound probiotic agent and a fermentation aid in water, spraying the dissolved compound probiotic agent and the fermentation aid into the raw materials, adding a probiotic agent for mixing, quantitatively sub-packaging the mixed raw materials into breathing bags, and performing solid-state fermentation at the fermentation temperature of 25-40 DEG C for 3-7 days to obtain the aquatic product fermented feed. The method has the effect of improving the feed utilization rate.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a preparation method and application of an aquatic fermented feed containing a composite probiotic agent. Background Art

[0002] Excessive breeding density and frequency, abuse of various chemical water products and antibiotics, large-scale input and residue of high-protein feed, large accumulation of aquatic animal excrement and the reproduction of harmful microorganisms will cause the breeding water to deteriorate. The discharge of polluted water sources will eventually lead to the weakening of the self-purification capacity of the breeding area environment, water quality, soil, etc. Fermented feed is an emerging safe and green product. Compared with traditional feed, it has its own unique advantages. It can not only regulate the microecological balance of aquatic animals, promote the healthy growth of animals, and improve production performance, but also regulate and protect the ecological environment of water bodies. Summary of the invention

[0003] The purpose of the present invention is to provide a method for preparing aquatic fermented feed containing a composite probiotic agent and application thereof, which has the effect of improving feed utilization.

[0004] The above technical objectives of the present invention are achieved through the following technical solutions: comprising the following steps:

[0005] (1) Bacillus amyloliquefaciens, Streptococcus lactis and yeast are mixed in proportion to form a composite probiotic agent;

[0006] (2) dissolving the composite probiotic agent and fermentation aid in water, spraying them into the raw materials, adding Yijunbao to mix, and then quantitatively packaging the mixed raw materials into breathing bags for solid-state fermentation at a fermentation temperature of 25° C. to 40° C. for 3 to 7 days to obtain aquatic fermented feed.

[0007] The present invention is further configured as follows: the Bacillus amyloliquefaciens, the lactic acid streptococcus and the yeast are mixed in a mass ratio of 1:3:1.

[0008] The present invention is further configured as follows: the raw materials are at least two of crushed corn, soybeans, soybean meal, bran, and vegetable cakes.

[0009] The present invention is further configured as follows: the addition amount of the composite probiotic agent is 1% to 3% of the mass of the raw material.

[0010] The present invention is further configured as follows: the fermentation aid is micro-doped produced by Hebei Yi Yang Biotechnology Co., Ltd.

[0011] The present invention is further configured as follows: the Yijunbao is produced by Hebei Yiyang Biotechnology Co., Ltd.

[0012] The present invention is further configured as follows: the total acid of the aquatic fermented feed is greater than 1%.

[0013] The present invention is further configured as follows: the aquatic fermented feed is added into aquaculture feed according to a weight percentage of 30%-50%.

[0014] The present invention is further configured as follows: according to the nutritional requirements of different aquatic animals, the fermented aquatic feed is crushed to a particle size of 1.0 mm, 1.5 mm, 2.5 mm or 4.5 mm.

[0015] The beneficial effects of the present invention are:

[0016] 1. The present invention improves feed utilization rate through solid-state fermentation; after fermentation, the feed produces a natural sour aroma, which can stimulate the appetite of animals, promote the secretion of digestive juices, increase the activity of digestive enzymes, accelerate the decomposition of feed nutrients, and thus promote the absorption of nutrients, so that the feed intake of fish and shrimps and the feed utilization rate are greatly improved.

[0017] 2. The present invention can improve the immunity and disease resistance of aquatic animals; after the feed is fermented by lactic acid bacteria, it becomes acidic, which can greatly reduce the number of harmful bacteria such as Salmonella and Escherichia coli, and improve the safety of the feed; fermented feed can inhibit harmful bacteria in the intestines of aquatic animals and enhance the disease resistance of aquatic animals.

[0018] 3. The present invention can improve the water environment; after feeding the fermented feed fermented by lactic acid bacteria, the harmful substances in the intestines of aquatic animals are decomposed by lactic acid, and the harmful substances in the feces of aquatic animals are reduced, so the deterioration of the water environment caused by the production of organic matter in the water by the feces can be greatly reduced. Due to the particularity of aquaculture, a variety of beneficial bacteria are put into the water body, and through oxidation, ammoniation, nitrification, denitrification and nitrogen fixation, a large amount of organic matter accumulated in the water (such as residual bait, excrement of farmed animals) and toxic and harmful gases (such as ammonia, hydrogen sulfide, etc.) produced by animal and plant remains can be quickly degraded. In the degradation process, a variety of inorganic salts that can be directly fully utilized by phytoplankton in the water can be produced. The phytoplankton that reproduces in large quantities in the water can further degrade harmful substances, improve various indicators of the aquaculture water body, and thus purify the water quality.

[0019] 4. The present invention can shorten the aquatic animal breeding cycle and reduce costs; the use of fermented feed fermented by lactic acid bacteria can increase the feed intake of aquatic animals, and the growth of aquatic animals in the later stage is relatively faster, shortening the breeding cycle; the various live microorganisms in the fermented feed can absorb a large amount of organic nitrogen that is difficult for animals to use, and convert it into a variety of bacterial proteins, namely protein feed, which significantly increases the nutritional content of the feed and reduces the feed cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1 is a picture of the first embodiment of the present invention;

[0022] Figure 2 This is a picture of the second embodiment of the present invention;

[0023] Figure 3 This is a picture of Embodiment 3 of the present invention;

[0024] Figure 4 This is a picture of the fourth embodiment of the present invention;

[0025] Figure 5 This is a picture of Embodiment 5 of the present invention. DETAILED DESCRIPTION

[0026] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] 1. Preparation of aquatic fermented feed

[0028] (1) Bacillus amyloliquefaciens, Streptococcus lactis, yeast and protease are mixed in a mass ratio of 1:3 and 1:0.2;

[0029] (2) The raw materials corn, soybeans, soybean meal, bran, and vegetable cakes are crushed and sieved into the required particle sizes. The particle sizes after sieving are 0.5 mm, 1 mm, 1.5 mm, 2 mm, and 2.5 mm, respectively.

[0030] (3) Dissolve the composite probiotic agent in water according to 1% of the mass of the raw material and an appropriate amount of fermentation aid, spray it into the raw material through an air pump, add Yijunbao and mix it using a closed tank mixer for 10 to 20 minutes, then pack the mixed raw materials into breathing bags through a quantitative valve, seal them, and carry out solid-state fermentation at a fermentation temperature of 25°C to 40°C for 3 to 7 days to obtain aquatic fermented feed.

[0031] The protein content (dry basis) in aquatic fermented feed is 10%-35%.

[0032] The fermentation aid is Microdo (the main ingredients are trace elements and vitamins, etc.) produced by Hubei Fengzhiyi Biotechnology Co., Ltd.

[0033] Yijunbao is produced by Hubei Fengzhiyi Biotechnology Co., Ltd. (the main ingredients are probiotic culture media such as brown sugar, glucose, and molasses powder).

[0034] 2. Experimental data

[0035] Table 1 Statistics on the actual use of fermented feed

[0036]

[0037]

[0038] According to Table 1, it can be seen that adding fermented feed to aquaculture feed can significantly improve the growth of river crabs, catfish and yellow catfish, promote the absorption of nutrients by aquatic organisms, and enhance the disease resistance of aquatic animals.

[0039] The above description is only a description of the preferred embodiments of the present invention, and is not intended to limit the scope of the present invention. Any changes or modifications made by a person skilled in the art in the field of the present invention based on the above disclosure shall fall within the scope of protection of the claims.

Claims

1. A method for preparing aquatic fermented feed containing a composite probiotic agent, characterized in that: The steps include: (1) Bacillus amyloliquefaciens, Streptococcus lactis and yeast are mixed in proportion to form a composite probiotic agent; (2) dissolving the composite probiotic agent and fermentation aid in water, spraying them into the raw materials, adding Yijunbao to mix, and then quantitatively packaging the mixed raw materials into breathing bags for solid-state fermentation at a fermentation temperature of 25° C. to 40° C. for 3 to 7 days to obtain aquatic fermented feed.

2. The method for preparing aquatic fermented feed containing a composite probiotic agent according to claim 1, characterized in that: The amyloliquefaciens bacillus, the lactic acid streptococcus and the yeast are mixed in a mass ratio of 1:3:

1.

3. The method for preparing aquatic fermented feed containing a composite probiotic agent according to claim 1, characterized in that: The raw materials are at least two of crushed corn, soybeans, soybean meal, bran and vegetable cake.

4. The method for preparing aquatic fermented feed containing a composite probiotic agent according to claim 2, characterized in that: The added amount of the composite probiotic agent is 1% to 3% of the mass of the raw material.

5. The method for preparing aquatic fermented feed containing a composite probiotic agent according to claim 1, characterized in that: The fermentation aid is Weiduo produced by Hubei Fengzhiyi Biotechnology Co., Ltd.

6. The method for preparing aquatic fermented feed containing a composite probiotic agent according to claim 1, characterized in that: The Yijunbao is produced by Hubei Fengzhiyi Biotechnology Co., Ltd.

7. The method for preparing aquatic fermented feed containing a composite probiotic agent according to claim 1, characterized in that: The total acid of the aquatic fermented feed is greater than 1%.

8. Use of aquatic fermented feed prepared by the method for preparing aquatic fermented feed containing a composite probiotic agent according to any one of claims 1 to 7, characterized in that: The aquatic fermented feed is added into aquaculture feed according to 30%-50% by weight.

9. The method for preparing aquatic fermented feed containing a composite probiotic agent according to claim 8, characterized in that: According to the nutritional requirements of different aquatic animals, the fermented aquatic feed is crushed to a particle size of 1.0 mm, 1.5 mm, 2.5 mm or 4.5 mm.

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