Novel microbial feed additive and method of producing the same

A microbial feed and additive technology, applied in animal feed, animal feed, bacteria, etc., can solve the problem of high cost, achieve the effect of increasing lean meat rate, improving feed remuneration, and improving the quality of meat products

Active Publication Date: 2008-10-08
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to use Lactobacillus plantarum and Enterococcus faecalis capable of converting linoleic acid into conjugated linoleic acid to make feed additives, so as to solve the problem of high cost caused by directly adding conjugated linoleic acid

Method used

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  • Novel microbial feed additive and method of producing the same
  • Novel microbial feed additive and method of producing the same
  • Novel microbial feed additive and method of producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The detection method of conjugated linoleic acid in the fermentation broth of Lactobacillus plantarum and Enterococcus faecalis is as follows:

[0024] 1) Liquid activation: Lactobacillus plantarum and Enterococcus faecalis were respectively inoculated in MRS liquid medium, and cultured statically at 37°C for 20 hours to make it in the mid-to-late logarithmic period; the MRS liquid medium (g / L): glucose 20; tryptone 10; beef extract 10; yeast extract 5; diammonium citrate 2; dipotassium hydrogen phosphate 2; MgSO 4 ·7H 2 O 0.58; MnSO 4 4H 2 O 0.25; Tween 801 mL.

[0025] Preparation method: MgSO 4 ·7H 2 O, MnSO 4 4H 2 O, dissolve the components other than glucose and Tween 80, cool to 50°C, adjust the pH value to 6.0-6.5 with acetic acid, and then add MgSO 4 ·7H 2 O, MnSO 4 4H 2 O, add glucose and Tween 80 at the end. Autoclave at 121°C for 20 minutes.

[0026] 2) Liquid fermentation and the measurement of conjugated linoleic acid: inoculate the seed solution...

Embodiment 2

[0028] Use a 50L fermenter to carry out liquid submerged high-density fermentation of Lactobacillus plantarum and Enterococcus faecalis, the steps are as follows:

[0029] 1) slant activation: Lactobacillus plantarum and Enterococcus faecalis strains were respectively transferred to eggplant bottle slant medium, and cultured at 37°C for 20h to make it in the late logarithmic period; the slant medium was MRS (g / L): glucose 20; tryptone 10; beef extract 10; yeast extract 5; diammonium citrate 2; dipotassium hydrogen phosphate 2; MgS0 4 ·7H 2 O 0.58; MnSO 4 4H 2 O0.25; Tween 801mL; agar 20.

[0030] Preparation method: MgSO 4 ·7H 2 O, MnSO 4 4H 2 O, dissolve the components other than glucose and Tween 80, cool to 50°C, adjust the pH value to 6.0-6.5 with acetic acid, and then add MgSO 4 ·7H 2 O, MnSO 4 4H 2 O, add glucose and Tween 80 at the end. Autoclave at 121°C for 20 minutes.

[0031] 2) Preparation of bacterial suspension: The seeds cultivated to the mid-to-la...

Embodiment 3

[0034] Post-treatment was carried out by spray drying. Centrifuge and concentrate the fermented liquid that has passed the inspection, and the obtained bacteria are used as the core material, sodium alginate is used as the wall material, Tween-80 and Span-80 are used as emulsifiers, the emulsifier dosage is 1%, and the homogeneous pressure is 30MPa , the feed temperature is 20°C; the inlet temperature is 110°C, and the outlet temperature is 45°C. Finally, the quality inspection is carried out, and after passing the test, it is packaged and put into storage.

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Abstract

The invention relates to a novel microorganism feed additive, in particular to a microorganism feed additive which contains bacterial strain that can produce conjugated linoleic acid and a preparation method thereof, which belongs to the microorganism feed additive technical field. The preparation method of the microorganism feed additive is that plant lactobacillus and enterococcus faecalis are respectively processed by zymotic liquid which is cultured by liquid deep-layer high-density fermenting and are made into powder by a micro-capsule peridum spray drying method and a frozen-dried preparation method. The microorganism feed additive transforms linoleic acid into the conjugated linoleic acid in the intestinal tracts of poultry and livestock, which takes effects of promoting the growth performance of the poultry and the livestock, reducing fat, increasing meat factor and regulating immunity reaction, etc., and the microorganism feed additive also solves the problem of the high expense caused by adding the conjugated linoleic acid into the feed directly. The microorganism feed additive prepared by the method has the advantages of high viable bacteria content, long holding period and little microbial pollution.

Description

technical field [0001] The invention belongs to the technical field of microbial feed additives, and particularly relates to a novel microbial feed additive and a preparation method thereof. The microorganisms used in the microbial feed additive can convert linoleic acid into conjugated linoleic acid. Background technique [0002] Antibiotics have been used as feed additives for 50 years, and have made great contributions to the growth and health of animals, but at the same time, they have brought many potential threats to human health and the development of the feed industry, mainly manifested in the production of antibiotics by animal pathogens. Drug properties, leading to an increase in the amount of antibiotics, causing endogenous infection and superinfection. Especially long-term use can cause microecological imbalance and environmental pollution in the digestive tract itself. At the same time, the residues of antibiotics in products also directly affect human immunity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16A23K1/00C12N1/20A23K10/18A23K50/30
Inventor 张玳华孙占敏莫云赵雁青宋维平
Owner BEIJING DABEINONG TECH GRP CO LTD
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