Method for preventing and/or treating infections, colonisations, or illnesses related to staphylococcus aureus, pseudomonas aeruginosa, streptococcus pyogenes, enterococcus faecium, enterobacter cloacae, proteus mirabilis, bacteroides fragilis, staphylococcus epidermidis, propionibacterium acnes, candida albicans and/or malassezia furfur

A streptococcal, colonization technique for the prophylaxis and/or treatment of Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pyogenes, Enterococcus faecium, Enterobacter cloacae, Proteus mirabilis, Bacteroides fragilis, Epidermal Staphylococcus, Propionibacterium acnes, Candida albicans, and/or Malassezia furfur-associated infection, colonization, or disease domain, capable of addressing pathogenic microbial colonization, commensal flora imbalance, etc.

Active Publication Date: 2015-12-23
URGO RECH INNOVATION & DEVEMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] - These antibiotics often have a considerable spectrum of action, which may create an imbalance in the commensal flora and thus cause colonization by pathogenic microorganisms;

Method used

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  • Method for preventing and/or treating infections, colonisations, or illnesses related to staphylococcus aureus, pseudomonas aeruginosa, streptococcus pyogenes, enterococcus faecium, enterobacter cloacae, proteus mirabilis, bacteroides fragilis, staphylococcus epidermidis, propionibacterium acnes, candida albicans and/or malassezia furfur
  • Method for preventing and/or treating infections, colonisations, or illnesses related to staphylococcus aureus, pseudomonas aeruginosa, streptococcus pyogenes, enterococcus faecium, enterobacter cloacae, proteus mirabilis, bacteroides fragilis, staphylococcus epidermidis, propionibacterium acnes, candida albicans and/or malassezia furfur
  • Method for preventing and/or treating infections, colonisations, or illnesses related to staphylococcus aureus, pseudomonas aeruginosa, streptococcus pyogenes, enterococcus faecium, enterobacter cloacae, proteus mirabilis, bacteroides fragilis, staphylococcus epidermidis, propionibacterium acnes, candida albicans and/or malassezia furfur

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0124] Example 1: Bacterial strain LactobacillussaniviriF3C5p (CNCMI-4650), Lactobacillus salivarius F50C2p (CNCMI-4651), Lactobacillus salivarius F52C3p (CNCM I-4652), Lactobacillus salivarius F41C3p (CNCMI-4653), Streptococcus light type F3C2v (CNCMI-4654) and Lactobacillus pentosus or Lactobacillus plantarum L1C1 (CNCMI-4655) Antibacterial activity

[0125] Lactobacillus saniviri F3C5p (CNCMI-4650), Lactobacillus salivarius F50C2p (CNCMI-4651), Lactobacillus salivarius F52C3p (CNCMI-4652), Lactobacillus salivarius F41C3p (CNCMI) were cultured in ManRogosaSharpe (MRS) medium at 37°C under anaerobic conditions -4653), Streptococcus light F3C2v (CNCMI-4654) and Lactobacillus pentosus or Lactobacillus plantarum L1C1 (CNCMI-4655) for 16 hours. Place 10 μl of these culture droplets on the surface of tryptone agar (TSA) medium. The tryptone agar (TSA) medium uses methicillin-resistant Staphylococcus aureus (MRSA) ATCC43300 or Pseudomonas aeruginosa ATCC9027 or Bacteroides fragil...

Embodiment 2

[0131] Example 2: Bacterial strain LactobacillussaniviriF3C5p (CNCMI-4650), Lactobacillus salivarius F50C2p (CNCMI-4651), Lactobacillus salivarius F52C3p (CNCM I-4652), Lactobacillus salivarius F41C3p (CNCMI-4653), Streptococcus light type F3C2v (CNCMI-4654) and Lactobacillus pentosus or Lactobacillus plantarum L1C1 (CNCMI-4655) Antibacterial activity after adding dressing

[0132] F3C5p, F50C2p, F52C3p, F41C3p, F3C2v and L1C1 bacteria were cultured in ManRogosaSharpe (MRS) medium at 37°C under anaerobic conditions for 16 hours. The dressing sheet (1cm×1cm) composed of polyurethane foam and lipid water glue net was used with 500μl of the culture solution of the bacteria according to the present invention (concentration of 10 9 -10 10 CFU.ml -1 ) Soaked and placed on the surface of tryptone agar (TSA) medium. The tryptone agar (TSA) medium is used for 10 6 -10 7 Two Staphylococcus aureus MRSAATCC43300 or Pseudomonas aeruginosa ATCC9027 cells were inoculated in advance in agar m...

Embodiment 3

[0134] Example 3: Attachment ability of bacteria according to the present invention on collagen

[0135] EpiSkin (SkinEthic) type recombinant epidermis (1.07cm 2 Lactobacillus saniviri F3C5p, Lactobacillus salivarius F50C2p, Lactobacillus salivarius F52C3p, Lactobacillus salivarius F41C3p, Streptococcus light type F3C2v and Lactobacillus pentosus or Lactobacillus plantarum L1C1 bacteria were attached to the test. The insert containing the epidermis was placed in a 12-well plate containing 2 ml of maintenance medium, and incubated at 35°C±2°C for 24 hours to regenerate the epidermis.

[0136] After incubation, the maintenance medium was removed and 2ml of MRS medium was added. Then use the bacteria according to the invention (with a concentration of approximately 2.5x10 7 CFU.ml -1 ) Inoculate a medium that simulates wound exudate, called simulated wound fluid (50 / 50vol / vol) (described in Werthén et al., 2010). After incubating for 24 hours, wash with saline to remove unattached b...

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Abstract

The subject matter of the present invention is a bacteria or mix of bacteria having an antagonistic activity with respect to stains of S. aureus, P. aeruginosa, Streptococcus pyogenes, Enterococcus faecium, Enterobacter cloacae, Proteus mirabilis, Bacteroides fragilis Staphylococcus epidermidis, Propionibacterium acnes, Candida albicans and/or Malassezia furfur as well as the use thereof in the treatment and/or prevention of infections or colonisations related to those pathogens. The invention pertains to care products containing one or more non-pathogenic antagonistic strains intended to prevent and/or treat infections or colonisations on skin, wounds, mucous membranes and appendages.

Description

Technical field [0001] The present invention relates to bacteria having antagonistic activity against pathogenic bacteria or yeasts (hereinafter referred to as "antagonistic bacteria") and their use as active ingredients or in medical equipment, especially in the treatment and / or prevention of these pathogenic bacteria Or yeast-related colonization and / or use in infections, the pathogenic bacteria or yeasts belong to the following genera and species: Staphylococcus aureus, Pseudomonasaeruginosa, Streptococcus pyogenes , Enterococcusfaecium, Enterobactercloacae, Proteusmirabilis, Bacteroidesfragilis, Staphylococcusepidermidis, Propionibacterium acnes, Candidaalbicans And / or Malasseziafurfur. Bacteria that are antagonistic to pathogenic bacteria or yeast are selected because they can inhibit the attachment and development and / or proliferation of pathogenic bacteria or yeast, thereby stabilizing and / or mediating the ecosystem. The present invention relates to a care product conta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61K35/744A61K45/06A61K8/99A61P31/04A61P31/10A61P37/02A61L15/44C12R1/225C12R1/25C12R1/46
CPCA61K35/747A61P17/02A61P31/02C12N1/205C12R2001/225C12R2001/25C12R2001/46A61K35/741A61K35/744A61K36/06A61K45/06
Inventor N·德罗什P·阿尔博J·居佐S·罗德里格斯C·洛朗苏S·梅里L·阿佩尔
Owner URGO RECH INNOVATION & DEVEMENT
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