Composite microorganism preparation and preparation method thereof

A technology of compounding microorganisms and preparations, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of destroying the normal flora of animal intestines, drug residues, emergence of drug-resistant strains, etc., and achieve excellent fermentation characteristics. , The effect of promoting animal growth and good stability

Inactive Publication Date: 2016-10-05
HENAN HUITONGTIANXIA ANIMAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As drugs or feed additives, antibiotics have greatly promoted the development of animal husbandry. However, antibiotics are widely used in animal husbandry, especially as feed additives. The disadvantages of antibiotics are becoming more and more obvious, such as drug residues, emergence of drug-resistant strains, and destruction of animal intestines. Disadvantages such as normal flora

Method used

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  • Composite microorganism preparation and preparation method thereof
  • Composite microorganism preparation and preparation method thereof
  • Composite microorganism preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A composite microbial preparation, comprising the following active components: at least two of Enterococcus faecalis, Saccharomyces cerevisiae, and Bacillus subtilis, as well as zymosan and cornstarch.

[0037] It consists of the following raw materials in parts by weight: 0.1-15 parts of Enterococcus faecalis powder, 0.1-15 parts of Saccharomyces cerevisiae powder, 0.1-10 parts of Bacillus subtilis powder, 0.1 part of zymosan, and 59.9-74.9 parts of corn starch.

[0038] Specific steps are as follows:

[0039] (1) Culture of Enterococcus faecalis:

[0040] a. Activation of strains: activate the strains stored in glycerol on slant medium, and culture them at 37°C for 24 h;

[0041] b. Preparation of seed medium: The components of the medium include: peptone 20 g / L, yeast powder 5.0 g / L, glucose 20 g / L, Tween-80 1.0 mL / L, dipotassium hydrogen phosphate 2.0 g / L , triammonium citrate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, sodium acetate 2.0 g / L; ad...

Embodiment 2

[0062] A complex microbial preparation comprising the following active components: Enterococcus faecalis, Saccharomyces cerevisiae, zymosan and cornstarch.

[0063] Specific steps are as follows:

[0064] (1) Culture of Enterococcus faecalis:

[0065] a. Activation of strains: activate the strains stored in glycerol on slant medium, and culture them at 37°C for 24 h;

[0066] b. Preparation of seed medium: The components of the medium include: peptone 20 g / L, yeast powder 5.0 g / L, glucose 20 g / L, Tween-80 1.0 mL / L, dipotassium hydrogen phosphate 2.0 g / L , triammonium citrate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, sodium acetate 2.0 g / L; adjust the pH to 7.0 with sodium hydroxide solution or dilute hydrochloric acid, and sterilize at 121 °C for 20 min , after the medium is cooled, it can be used as a primary seed medium;

[0067] c. Prepare the first-grade seed solution: use the inoculation loop to pick the activated strains and inoculate them into t...

Embodiment 3

[0081] A complex microbial preparation comprising the following active components: Enterococcus faecalis, Saccharomyces cerevisiae, zymosan and cornstarch.

[0082] Specific steps are as follows:

[0083] (1) Culture of Enterococcus faecalis:

[0084] a. Activation of strains: activate the strains stored in glycerol on slant medium, and culture them at 37°C for 24 h;

[0085] b. Preparation of seed medium: The components of the medium include: peptone 20 g / L, yeast powder 5.0 g / L, glucose 20 g / L, Tween-80 1.0 mL / L, dipotassium hydrogen phosphate 2.0 g / L , triammonium citrate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, sodium acetate 2.0 g / L; adjust the pH to 7.1 with sodium hydroxide solution or dilute hydrochloric acid, and sterilize at 121 °C for 20 min , after the medium is cooled, it can be used as a primary seed medium;

[0086] c. Prepare the first-grade seed solution: use an inoculation loop to pick the activated strains and inoculate them into the ...

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Abstract

The invention discloses a composite microorganism preparation including the active components as follows: at least two of enterococcus faecalis, saccharomyces cerevisiae and bacillus subtilis, and as well as zymosan and corn starch. The preparation is composed of, by weight, 0.1-15 parts of powder of the enterococcus faecalis, 0.1-15 parts of powder of the saccharomyces cerevisiae, 0.1-10 parts of powder of the bacillus subtilis, 0.1 parts of the zymosan and 59.9-74.9 parts of the corn starch. Compared with the prior art, the enterococcus faecalis, saccharomyces cerevisiae and bacillus subtilis are organically combined so that advantages of the bacteria can be achieved completely. The preparation can adapt different hosts and conditions, can partially, even completely replace antibiotics, and can regulate ecologic balance in gastrointestinal tracts, promote growth, increase conversion rate of feeds and enhance immunity. The preparation can improve enteric microorganism environment in animals, inhibit growth of harmful bacteria and promote reproduction of beneficial bacteria, improves animal immunity and enhances disease resistance, supplies nutrients to animals, improves utilization rate of feed and promotes growth of the animals.

Description

technical field [0001] The invention belongs to the technical field of microbial feed, and in particular relates to a compound microbial preparation and a preparation method thereof. Background technique [0002] As drugs or feed additives, antibiotics have greatly promoted the development of animal husbandry, but antibiotics are widely used in animal husbandry, especially as feed additives, and their various disadvantages are becoming more and more obvious, such as drug residues, emergence of drug-resistant strains, and destruction of animal intestines. Disadvantages such as normal flora. In order to overcome the disadvantages of antibiotics, countries all over the world have strengthened the research and application of new green feed additives, especially microbial feed additives have attracted people's attention and developed rapidly. [0003] Enterococcus faecalis ( Enterococcus faecalis ) is a Gram-positive bacterium, which was listed as Enterococcus in the "Bergey's ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18A23K1/16A23K1/18C12R1/865C12R1/125C12R1/01
Inventor 张要齐宋志元张新蕾朱静静
Owner HENAN HUITONGTIANXIA ANIMAL MEDICINE
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