Chicken feed probiotic agent containing enterococcus faecalis and preparation method of chicken feed probiotic agent

A technology of Enterococcus faecalis and probiotics, applied in biochemical equipment and methods, methods based on microorganisms, animal feed, etc., can solve the problems of probiotic strain degradation and low biological activity

Active Publication Date: 2015-01-21
BEIJING HESWOF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of serious degradation of probiotic strains and low biological activi

Method used

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  • Chicken feed probiotic agent containing enterococcus faecalis and preparation method of chicken feed probiotic agent
  • Chicken feed probiotic agent containing enterococcus faecalis and preparation method of chicken feed probiotic agent
  • Chicken feed probiotic agent containing enterococcus faecalis and preparation method of chicken feed probiotic agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 raw material preparation

[0050] 1. Preparation of Enterococcus faecalis Active Sludge

[0051] The preparation method of described Enterococcus faecalis active bacteria slime is as follows:

[0052] Pick 2 loops of the activated Enterococcus faecalis HEW-A131 slant strain and inoculate it in the liquid seed medium at 35°C and 180r / min for 4 hours to obtain a viable bacterial concentration of 10 9 CFU / mL seed solution; take 3mL seed solution and inoculate it in a 300mL shake flask seed medium and cultivate it for 6h at 35°C and 180r / min to obtain a shake flask seed solution; take 0.30L shake flask seed solution and inoculate it in a 30L shake flask seed culture medium In a 50L fermenter based on the base, ferment at 35°C and 110r / min for 4h to obtain the seed liquid of the fermenter; take the seed liquid of the 30L fermenter and inoculate it into a 5000L fermenter with 3000L of fermentation medium, culture at 35°C and 90r / min 8h to obtain the fermented l...

Embodiment 2

[0075] A kind of chicken feed probiotic containing Enterococcus faecalis, its preparation method comprises the steps:

[0076] Put Enterococcus faecalis active bacteria sludge, Bacillus subtilis active bacteria sludge, Saccharomyces cerevisiae active bacteria sludge and stabilizing protective agent in the mixing tank at a mass ratio of 0.5:1.8:0.3:1, start the agitator, and mix evenly for 22 minutes, Stir at a speed of 50r / m, then add water to adjust the humidity of the mixture to 33% to obtain microcystis wet powder; put the microcystis wet powder into a granulator, adjust the particle size to 30 mesh, and then put it into a swirl fluidized bed for granulation Drying in the coating machine, the drying inlet temperature is 60°C, the outlet air temperature is controlled at 30°C, and the drying time is 60min; finally, the side spray coating device is started, the inlet air temperature is controlled at 55°C, and the atomization pressure is 0.15MPa. The agent solution is sprayed t...

Embodiment 3

[0079] A kind of chicken feed probiotic containing Enterococcus faecalis, its preparation method comprises the steps:

[0080] Put Enterococcus faecalis active bacteria sludge, Bacillus subtilis active bacteria sludge, Saccharomyces cerevisiae active bacteria sludge and stabilizing protective agent in the mixing tank at a mass ratio of 0.1:1:0.1:1, start the agitator, and mix evenly for 15 minutes, Stir at a speed of 60r / m, then add water to adjust the humidity of the mixture to 32%, to obtain microcystis wet powder; put the microcystis wet powder into the granulator, adjust the particle size to 20 mesh, and then put it into the swirling fluidized bed for granulation Dry in the coating machine, the drying air temperature is 55°C, the air outlet temperature is 28°C, and the drying time is 50min; finally start the side spray coating device, the air inlet temperature is controlled at 45°C, the atomization pressure is 0.1MPa, and the coating agent The solution was sprayed to the m...

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Abstract

The invention discloses a chicken feed probiotic agent containing enterococcus faecalis and a preparation method of the chicken feed probiotic agent. The chicken feed probiotic agent is prepared by virtue of the steps of evenly mixing enterococcus faecalis HEW-A131 active bacterial sludge, bacillus subtilis active bacterial sludge, saccharomyces cerevisiae active bacterial sludge and a stabilization protective agent together in the mass ratio of (0.1-0.8): (1-2.5): (0.1-0.5): 1, and then pelletizing, drying, coating and the like; the probiotic agent is capable of improving the utilization efficiency of an animal feed and saving the cost, and also capable of greatly improving the steady state of the internal environment of the digestive tract in an animal body, promoting the absorption and utilization of nutrition and promoting the growth of the animal; as a result, the productivity, the immune performance and the reproductive performance of the animal are obviously improved, the production cost is reduced and the economic benefit is increased.

Description

technical field [0001] The invention relates to a probiotic agent for chicken feed, in particular to a probiotic agent for chicken feed containing Enterococcus faecalis and a preparation method thereof. Background technique [0002] In the modern breeding industry, the addition of antibiotics to feed began in the 1950s, and it plays an important role in preventing and controlling diseases, promoting growth, saving feed, and improving economic benefits of farming. However, with the long-term addition of antibiotics and chemically synthesized drugs in large quantities in the feed, pathogenic microorganisms in animals have developed drug resistance during breeding production, the application effect has decreased, and production costs have increased; animals have drug dependence and their own immune function Inhibition, decreased immunity and disease resistance; beneficial bacteria in animals are inhibited, flora imbalance, forming endogenous infection; drug residues in animal p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18A23K1/16A23K1/18C12R1/125C12R1/46C12R1/865
CPCC12N1/18C12N1/20
Inventor 李雪平
Owner BEIJING HESWOF BIOTECH CO LTD
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