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A strain of Enterococcus faecium hew‑a588 and its application

A technology of HEW-A588 and Enterococcus faecium, applied in the field of microbiology, can solve the problems of Enterococcus faecium heat resistance, acid and alkali resistance, antibacterial performance and fermentation performance are not ideal, and achieve significant probiotics and stress resistance Sexuality, good application prospect, strong resistance effect

Active Publication Date: 2018-02-16
BEIJING HESWOF BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing Enterococcus faecium is not ideal in heat resistance, acid and alkali resistance, antibacterial performance and fermentation performance, and cannot adapt to the growing industrialization needs, especially from the current goals of the feed industry and animal husbandry. Therefore, , the isolation and purification of probiotic Enterococcus faecium strains that are more suitable for the development of animal husbandry has become a technical problem to be solved urgently

Method used

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  • A strain of Enterococcus faecium hew‑a588 and its application
  • A strain of Enterococcus faecium hew‑a588 and its application
  • A strain of Enterococcus faecium hew‑a588 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Isolation, screening and identification of Enterococcus faecium HEW-A588

[0036] 1. Isolation and purification of Enterococcus faecium

[0037]Under aseptic conditions, 1 g of intestinal content of healthy piglets was collected, placed in a conical flask filled with 30 mL of MRS medium, enriched for 10 h, and then 1 mL of culture solution was drawn into a test tube filled with 9 mL of sterilized saline. This dilution is 10 -1 , diluted sequentially to 10 -6 , select 10 -3 ~10 -6 For three dilutions, draw 0.1mL and spread it on the KF Streptococcus agar plate, incubate the coated plate upside down at 37°C for 24-48 hours, and pick the dark red colonies with different shapes on the MRS solid medium with an inoculation loop Separation and culture were carried out on the plate. After 48 hours of culture, vigorously growing colonies were picked, transferred to MRS slant medium with an inoculation loop for pure culture, repeated subculture for 3 times, and stor...

Embodiment 2

[0060] Example 2 Preparation of Enterococcus faecium HEW-A588 probiotics

[0061] 1. Preparation of active bacteria sludge of Enterococcus faecium HEW-A588

[0062] 1.1 Preparation of Enterococcus faecium HEW-A588 fermentation broth

[0063] Take the glycerin tube of Enterococcus faecium HEW-A588 preserved in liquid nitrogen, dissolve it in a water bath at 37°C, separate and purify it by streaking on an MRS plate, and culture it at 37°C for 24-48 hours; pick and grow on a plate in a sterile room Vigorous single colony, inoculated on fresh MRS slant, cultured at 37°C for 12-18h; add 2.0mL sterilized physiological saline to each test tube in a sterile room, scrape the bacterial lawn with an inoculation loop, and combine to make a bacterial suspension; draw 0.8 mL of the bacterial suspension was inoculated into a shaker flask medium (300 mL / 1L Erlenmeyer flask), pH 6.8, 37° C., 150 r / min shaking culture for 6.5 h, to obtain a seed liquid.

[0064] After the shake flask fermenta...

Embodiment 3

[0091] Example 3 Mice challenge test

[0092] 180 ordinary Kunming mice were selected, weighing 18-20 g, half male and half male, and fed routinely. After raising for one week, randomly select 120 and divide into two groups, wherein 60 are as test group (probiotics group, prepared in embodiment 2), feed the feed (15g probiotics / 1kg feed) that contains probiotics every day, another 60 mice were used as the control group, fed with conventional feed, and fed freely for 21 days. The feces of the mice were collected every 5 days to measure the number of E. coli, and the growth of the mice was observed. Take about 0.1g of mouse feces, add a few glass beads (0.1g of feces plus 0.9mL diluent) into the sterile operating table, dilute and inoculate MacConkey agar medium, and calculate the E. coli per gram of wet feces number.

[0093] After feeding for 21 days, mice were injected intraperitoneally with Escherichia coli bacteria solution, 0.5mL / only, and the concentration of the bacter...

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Abstract

The present invention provides a newly isolated strain of Enterococcus faecium (Enterococcus faecium) HEW‑A588, which has remarkable probiotic and stress resistance, and the microecological preparation prepared by it enters the intestinal tract of animals, rapidly activates and proliferates, and can Forming beneficial dominant flora and maintaining intestinal balance can not only improve the feed utilization efficiency of animals and save costs, but also improve the stability of the environment in the digestive tract of animals, promote the absorption and utilization of nutrients, promote animal growth, and improve economic benefits, etc. , has a good application prospect.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a strain of Enterococcus faecium HEW-A588 and its application. Background technique [0002] Enterococcus faecium (Enterococcus faecium) is a normal flora in the intestinal tract of humans and animals. It grows and reproduces quickly. It is a Gram-positive streptococcus or coccus, mostly arranged in pairs or short chains, usually immobile, and without spores; Its colonies are round, milky white, with raised surfaces, neat edges, and moist luster. Enterococcus faecium can produce lactic acid and antibacterial substances, which can inhibit the reproduction of pathogenic bacteria and spoilage bacteria in the intestinal tract, reduce the rate of diarrhea and mortality, and at the same time promote digestion and absorption of animals, improve the micro-ecological balance in the intestinal tract, and increase food intake Feed conversion rate, improve immunity and protect animal health. En...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/46
CPCC12N1/20C12N1/205C12R2001/46
Inventor 李雪平
Owner BEIJING HESWOF BIOTECH CO LTD
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