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A kind of probiotics for pigs and preparation method thereof

A technology of probiotics and seeds, which is applied in the field of pig probiotics and its preparation, can solve the problems of low probiotic content affecting the use effect, inconsistent product standards, and unknown strain sources, so as to improve health and autoimmunity The effect of increasing the power, improving economic benefits, and reducing the incidence of sow postpartum gynecomastia

Active Publication Date: 2018-06-29
江西好实沃生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also many problems in the preparation technology of microecological preparations, such as inconsistent product standards, too high or too low content of probiotics, which affects the use effect; the source of the bacteria is unknown, easy to degrade, and low in activity; the compatibility and synergy of the bacteria are poor; the fermentation process is chaotic, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of Enterococcus faecium HEW-A588 Active Sludge

[0040] 1.1 Preparation of Enterococcus faecium HEW-A588 fermentation broth

[0041] Take the glycerin tube of Enterococcus faecium HEW-A588 preserved in liquid nitrogen, dissolve it in a water bath at 37°C, separate and purify it by streaking on an MRS plate, and culture it at 37°C for 24-48 hours; pick and grow on a plate in a sterile room Vigorous single colony, inoculated on fresh MRS slant, cultured at 37°C for 12-18h; add 2.0mL sterilized physiological saline to each test tube in a sterile room, scrape the bacterial lawn with an inoculation loop, and combine to make a bacterial suspension; draw 0.8 mL of the bacterial suspension was inoculated into a shaker flask medium (300 mL / 1L Erlenmeyer flask), pH 6.8, 37° C., 150 r / min shaking culture for 6.5 h, to obtain a seed liquid.

[0042] After the shake flask fermentation, carry out the fermenter pilot test, take 100mL shake flask fermentation see...

Embodiment 2

[0047] The preparation of embodiment 2 Bacillus licheniformis active bacteria slime

[0048] 2.1 Preparation of Bacillus licheniformis fermentation broth

[0049] Bacillus licheniformis was cultured in the order of slope, liquid seed and liquid fermentation. Take Bacillus licheniformis preserved on the slant and inoculate it into 300mL first-grade seed liquid medium, and carry out shake-flask fermentation culture. The fermentation temperature is 25-45°C, the initial pH is 7.1, 180r / min, the fermentation time is 8-20h, and the first-grade seed is obtained. Shake the bottle with seed solution. Transfer the primary seed solution to 35L secondary seed culture medium (50L fermenter), inoculum size is 1.5%, fermentation temperature is 35°C, initial pH value is 7.1, stirring speed is 140r / min, cultivated for 8h to obtain secondary seeds liquid, the secondary seed liquid was transferred to the tertiary fermentation medium (2×10 4 L fermentation tank), after inoculation, the tank te...

Embodiment 3

[0053] Example 3 Preparation of Yeast Active Sludge

[0054] 3.1 Preparation of yeast fermentation broth

[0055] The Pichia pastoris is cultivated in the order of slant surface, liquid seed and liquid fermentation. Take the Pichia pastoris preserved on the slant and inoculate it into 300mL first-class seed liquid medium, and carry out shake flask fermentation culture. The fermentation temperature is 30°C, pH 6.8, 120r / min, and the fermentation time is 10-15h, and the first-grade shake flask seeds are obtained. liquid. Transfer the primary seed liquid to 35L secondary seed medium (50L fermenter), inoculum size is 2%, fermentation temperature is 30°C, pH value is 6.8, stirring speed is 100r / min, cultivated for 9h to obtain secondary fermentation liquid , transfer the secondary seed solution to the tertiary fermentation medium (2×10 4 L fermentation tank), after inoculation, the temperature of the tank was controlled at 30°C, pH 6.8, the stirring speed was 120r / m, and culture...

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Abstract

The invention provides a novel probiotic agent for pigs and a preparation method thereof. The probiotic agent for pigs can not only improve the feed utilization efficiency of animals, save costs, but also greatly improve the stability of the environment in the digestive tract of animals, and promote The absorption and utilization of nutrients can promote the growth of animals, significantly improve the production performance, immune performance and reproductive performance of animals, reduce production costs, improve economic benefits, and have good application prospects.

Description

technical field [0001] The invention relates to a microecological preparation and a preparation method thereof, in particular to a probiotic agent for pigs and a preparation method thereof. Background technique [0002] The use of antibiotics has played an important role in preventing diseases, promoting growth, and improving economic benefits. At the same time, its side effects such as the emergence of superbugs, endogenous infections and secondary infections, the destruction of intestinal micro-ecological balance, and livestock and poultry products and Environmental residues have brought serious threats to animal husbandry and human health. Therefore, developing alternatives to antibiotics provides a reliable technical guarantee for green, safe and efficient feed additives. As a new type of natural bioactive additive, probiotics have the functions of antibiotics and enzymes, are non-toxic, non-resistant, and have no residue, and can improve the balance of intestinal flora...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/04C12N1/20C12N1/16A23K10/18C12R1/46C12R1/10C12R1/84
Inventor 李雪平
Owner 江西好实沃生物技术有限公司
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