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Feed level microbe additive and its prepn and application

A microbial additive and feed-grade technology, applied in the direction of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve problems such as complex operations, achieve the effects of simple preparation and operation, increase daily weight gain, and reduce feed-to-meat ratio

Inactive Publication Date: 2006-05-17
HANGZHOU HUAYANG AOBO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this patent requires the use of two combined probiotics during the growth of poultry, the operation is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035]Example 1: Preparation of Feed Grade Microbial Additive --- Batch Fermentation Method

[0036] Dissolve 0.2 g CaCl 2 and MgSO 4 In 800 ml of purified water, dissolve it completely and then add 1.0 g of K 2 HPO 4 , 1.0 g KH 2 PO 4 , 10.0 g NaHO 3 , 2.0 g of NaCl and 200 ml of purified water, and store at 4C after all dissolved.

[0037] Take 300 ml of the prepared PYG anaerobic medium respectively in a sealed Erlenmeyer flask. Lactobacillus acidophilus, Enterococcus faecalis and Clostridium butyricum were respectively inoculated (10% inoculum). After inoculation, the upper part of the triangular flask was filled with CO 2 gas, and then cultured statically at 37°C for 24 hours as primary seeds.

[0038] Then prepare 3 liters of PYG anaerobic medium according to the above method, and inoculate the primary seeds of Lactobacillus acidophilus, Enterococcus faecalis and Clostridium butyricum respectively. After inoculation, the upper part of the container is filled wi...

example 2

[0041] Example 2: Preparation of Feed Grade Microbial Additive --- Continuous Fermentation Method

[0042] Dissolve 0.2 g CaCl 2 and MgSO 4 In 800 ml of purified water, dissolve it completely and then add 1.0 g of K 2 HPO 4 , 1.0 g KH 2 PO 4 , 10.0 g NaHO 3 , 2.0 g of NaCl and 200 ml of purified water, and store at 4C after all dissolved.

[0043] Take 300 ml of the prepared PYG anaerobic medium respectively in a sealed Erlenmeyer flask. Lactobacillus acidophilus, Enterococcus faecalis and Clostridium butyricum were respectively inoculated (10% inoculum). After inoculation, the upper part of the triangular flask was filled with CO 2 gas, and then cultured statically at 37°C for 24 hours as primary seeds.

[0044] Then prepare 3 liters of PYG anaerobic medium according to the above method, and inoculate the primary seeds of Lactobacillus acidophilus, Enterococcus faecalis and Clostridium butyricum respectively. After inoculation, the upper part of the container is fil...

example 3

[0046] Example 3: Effects on Salmonella intestinal colonization of chicks

[0047] 50 feathers of 1-day-old "Zhijianghuang" were randomly divided into two groups, and each feather of the test group was fed with 0.3ml of the microbial additive obtained in Example 1 (dissolved 0.25g of probiotics in 25ml of nutrient solution, and then 0.3ml of each chick was taken. milliliter fed), and the control group was fed with 0.3ml sterilized water per feather. After 24 hours, each feather was fed with 0.3ml Salmonella enrichment solution (containing 10 7 Salmonella typhimurium). At the age of 10 days and 17 days, 13 and 30 feathers were randomly taken from the control group and the test group respectively, and the liver and spleen were streaked to determine the total number of bacteria, and the contents of the cecum were sampled for qualitative and quantitative detection of Salmonella.

[0048] time

[0049] The results showed that the anti-Salmonella ability of the test gro...

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PUM

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Abstract

The present invention provides one kind of feed level microbe additive and its preparation and application. The microbe additive includes mainly probiotics part and nutritious liquid part. The probiotics part contains acidophilic lactobacillus, fecal enterococcin and butynic clostridium; and the nutritious liquid part contains acetic acid, propanoic acid and butyric acid. The feed level microbe additive is taken by chickling in 24 hr after hatching, and this can reduce the use amount of antiseptic medicine obviously, raise daily weight gain, lower the feed / meat ratio and raise economic utility obviously. In addition, it has simple preparation and low cost.

Description

(1) Technical field [0001] The invention relates to a feed grade microbial additive and a preparation method thereof, as well as the application of the additive in poultry feeding. (2) Background technology [0002] In the past two decades, with the rapid growth of my country's animal husbandry production scale, my country has become the world's largest producer and trader of livestock and poultry products. Among them, poultry egg production ranks first in the world, poultry production ranks second in the world, and beef production ranks third in the world. Statistics from the Ministry of Agriculture show that in 2003, the total number of poultry slaughtered in my country reached 8.4 billion. However, the reality that is not commensurate with the large animal husbandry country is that the overall technical level of my country's animal husbandry industry is still very low, and bacterial infectious diseases are still mainly controlled by antibiotics, which can easily lead to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/105A23K50/75C12N1/00
Inventor 王欣曹郁生肖玉峰苏长城
Owner HANGZHOU HUAYANG AOBO BIOTECH
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