Microorganism vitamin premixed material and preparation method

A technology of vitamin premixes and microorganisms, applied in animal feed, animal feed, additional food elements, etc., can solve problems such as threats to human health, drug residues, lower market competitiveness and consumer trust, and achieve competitiveness and industrialization prospects, regulating animal micro-ecological balance, and improving the effect of animal non-specific immunity

Active Publication Date: 2009-02-18
BEIJING DABEINONG TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The addition of antimicrobials to the premix and the blind abuse of antimicrobials in the breeding process have led to a series of problems: First, the feed and aquaculture industries rely heavily on antimicrobials, which makes the resistance of pathogenic bacteria and viruses in animals and in the breeding environment Intensification of the drug properties, resulting in reduced drug efficacy and increased breeding costs, has led to the destruction of the ecological environment inside and outside the animal body, and increased the difficulty of animal disease prevention and control; secondly, it has led to serious drug residue problems in poultry and livestock products, and product quality and The dec

Method used

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  • Microorganism vitamin premixed material and preparation method
  • Microorganism vitamin premixed material and preparation method
  • Microorganism vitamin premixed material and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of bacillus

[0032] The seed medium is a mixed solution (g / L) prepared according to the following ratio: bran 50, fish meal 3.0, soybean meal 10, yeast extract 3.0, sodium hydroxide 2.0, boil water for 1 hour, and neutralize with hydrochloric acid to 6.8-7.0.

[0033] The fermentation medium is a mixed solution (g / L) prepared in the following proportions: bran 50, soybean meal 20, sodium carbonate 0.3, and antifoaming agent 0.4. Sterilized at 121°C for 30 minutes.

[0034] Liquid submerged aerated fermentation culture, seed culture for 8 hours, inoculum size 1%, culture temperature 36-37°C. The stirring speed is 280-300rpm, the tank pressure is 0.06MPa, the air flow rate is 1:0.4-0.6, the relative value of dissolved oxygen is controlled at 80%-90%, and the number of spores is detected after 16 hours of fermentation time. When a large number of spores are observed, put the tank. The fermented liquid is directly spray-dried to make bacillus...

Embodiment 2

[0035] Embodiment 2: the preparation of yeast

[0036] The seed medium is a mixed solution (g / L) prepared according to the following ratio: molasses 70-80, peptone 5-10, ammonium sulfate 1.0, magnesium sulfate heptahydrate 0.5; pH is natural pH, sterilized at 121°C for 30 minutes.

[0037] The fermentation medium is a mixed liquid (g / L) prepared according to the following ratio: molasses 70-80, urea 2-10, sodium chloride 1.0; pH is natural pH, sterilized at 121° C. for 30 minutes.

[0038] Liquid submerged fermentation culture, seed culture for 12 hours, inoculum size 3-6%, culture temperature 28-30°C. The stirring speed is 150-250rpm, the tank pressure is 0.06MPa, the air flow rate is 1:0.4-0.6, the relative value of dissolved oxygen is controlled at 80%-90%, and after 24 hours of fermentation time, the number of colonies is detected to be qualified and then put into the tank.

[0039] The fermented liquid is vacuum-concentrated at low temperature to 40% of the original volu...

Embodiment 3

[0041] Embodiment 3: the preparation of enterococcus

[0042] The seed medium is a mixed solution (g / L) prepared according to the following ratio: glucose 10-30, yeast extract 5-15, dipotassium hydrogen phosphate 2.0-5.0; potassium dihydrogen phosphate 2.0-5.0; pH is natural pH , sterilized at 121°C for 30 minutes.

[0043] The fermentation medium is a mixed solution (g / L) prepared in the following proportions: glucose: 10-30, yeast extract 2.0-10, sodium chloride 1.0. The pH is natural pH, sterilized at 121°C for 30 minutes.

[0044] For fermentation culture, seeds are cultivated for 12 hours, the inoculum size is 3-6%, and the culture temperature is 30-35°C. The fermentation time is 24 to 50 hours, and after the fermentation time is 24 hours, the number of colonies is detected to be qualified, and then the tank is released.

[0045] The fermented liquid is vacuum-concentrated at low temperature to 30% to 60% of the original volume, and a carrier is added to the concentrat...

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PUM

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Abstract

The invention relates to a microbe vitamin premix material and a method for preparing the same, in particular to a microbe vitamin premix material consisting of a plurality of useful microbes and vitamins and a method for preparing the same. The microbe vitamin premix material comprises bacillus, microzyme, enterococcus, vitamin A, vitamin D3, vitamin E, vitamin K3 and vitamin 12, and is the microbe vitamin premix material generally for pigs, poultries, ruminant or aquatic animals. The microbe vitamin premix material has the animal immunization health care function as well as nutrient efficacy, and can improve illness resistant capability of animals, reduce the consumption of antibacterial drugs in feed, lower the drug resistant degree of bacteria, improve the feed utilization ratio, promote health of mankind and improve the ecological environment.

Description

technical field [0001] The invention relates to the field of microbial application, in particular to a microbial vitamin premix and a preparation method thereof. Background technique [0002] The addition of preventive and growth-promoting drugs in feed and aquaculture has greatly promoted the development of my country's aquaculture industry. At present, more than 95% of domestic premix products are added with growth-promoting and preventive antibacterial drugs. The addition of antimicrobials in the premix and the blind abuse of antimicrobials in the breeding process have led to a series of problems: First, the feed and aquaculture industries rely heavily on antimicrobials, which makes the resistance of pathogenic bacteria and viruses in animals and in the breeding environment Intensification of the drug properties, resulting in reduced drug efficacy and increased breeding costs, has led to the destruction of the ecological environment inside and outside the animal body, and ...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/175A23K1/00A23K10/18A23K20/174
Inventor 张玳华赵雁青莫云宋维平孙占敏贾秋英白玉卿姚琨
Owner BEIJING DABEINONG TECH GRP CO LTD
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