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Method for producing natural carotenoids by scenedesmus obliquus culture

A technology of Scenedesmus obliquus and carotene is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of high safety, short production cycle and improved economic benefits

Inactive Publication Date: 2018-10-16
JINGCHU UNIV OF TECH
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  • Abstract
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for the production of natural carotenoids of Scenedesmus obliquus New by acclimating the new domesticated algae species of Scenedesmus obliquus New. Pollution (Wan Duanji et al., Patent No. 201310660674) and the development and utilization of the unique high antioxidant capacity of the new Scenedesmus obliquus (especially Scenecarotenoid), provide a solution to the problem of yellow pulp water treatment, process simplification using yellow pulp water culture The method for producing new natural carotenoid products from Scenedesmus obliques, the algae species used are selected and domesticated in this laboratory, and come from the School of Bioengineering, Jingchu Institute of Technology

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  • Method for producing natural carotenoids by scenedesmus obliquus culture
  • Method for producing natural carotenoids by scenedesmus obliquus culture

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Embodiment 1

[0026] Take JN-6001 low temperature and high pressure homogenizer for low temperature and high pressure homogenization and ethanol extraction as an example

[0027] The method for producing natural selenium-enriched natural carotenoids from Scenedesmus obliquus of the present invention includes the following steps: S1, yellow pulp water pretreatment: after yellow pulp water is collected during the production of soybean products, 5- Filter with 7 layers of gauze, use NaOH to adjust the pH value of the yellow slurry water filtrate to 7.5-8.0 after filtration;

[0028] S2. Preparation of selenium-enriched yellow syrup water medium: add 8%-12% selenium-enriched potato juice (from Enshi, "the world's selenium capital") and mass-volume ratio to the yellow syrup water filtrate prepared in S1 It is 25%-35% concentrated selenium-enriched spring water (from Enshi, "the world's selenium capital"). The mass ratio of yellow pulp water filtrate, selenium-enriched potato juice and concentrated se...

Embodiment 2

[0042] Take ultrasound assistance and ethanol extraction as an example

[0043] S1. Yellow pulp water pretreatment: After collecting the yellow pulp water during the production of soy products, filter it with 5-7 layers of gauze. After filtration, use NaOH to adjust the pH value of the yellow pulp water filtrate to 7.5-8.0;

[0044] S2. Preparation of selenium-enriched yellow pulp water medium: add 8%-12% selenium-enriched potato juice with a mass-volume ratio of 25%-35% in the yellow-paste water filtrate prepared in S1 The mass ratio of selenium spring water, yellow syrup water filtrate, selenium-enriched potato juice and concentrated selenium-enriched spring water is 7:2:1, and then sterilized by autoclaving at 121℃ for 30min to prepare selenium-enriched yellow syrup water medium ;

[0045] S3. Activated Scenedesmus obliquus: use the selenium-rich yellow slurry water medium obtained in S2 to cultivate and activate the Scenedesmus obliquus;

[0046] S4. Preliminary cultivation of al...

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Abstract

The invention provides a method for producing natural carotenoids by culturing of Scenedesmus obliquus New which is newly domesticated scenedesmus obliquus. The method includes that yellow serofluid pretreatment, pH adjustment, adding of rich-selenium culture (obtained from 'the world selenium city' Enshi), sterilization and lateral lighting culture with red-light lamp tubes on two sides are performed; carotenoid induction is carried out through methyl jasmonate, top-lighting blue light 7600lux in light-dark period of 21h:3h, temperature stimulation at 32-37 DEG C, nitrogen starvation, glucoseand xylose; algae cell low-temperature high-pressure homogenization wall breaking or ultrasonic-assisted extraction of carotenoids is performed to prepare a dried product of rich-selenium carotenoids. In consumption of per gram of scenedesmus obliquus in dry weight, 123.12mg of the dried product of Scenecarotenoid which is novel rich-selenium natural carotenoids can be obtained. The method is remarkably helpful to production of novel products of rich-selenium natural carotenoids, and a promising development prospect and an extremely high market value are achieved.

Description

Technical field [0001] The invention relates to the field of carotenoid extraction, in particular to a method for culturing Scenedesmus obliquus species to produce natural carotenoids. Background technique [0002] Carotenoids are proven to be one of the advanced nutritional products and medicines. They are used to maintain bone and tooth health, prevent osteoporosis, prevent and treat cataracts and macular degeneration, prevent and treat diabetes, prevent Alzheimer's disease, and prevent cardiovascular and cerebrovascular diseases. Diseases have outstanding health care functions (Wu Qingying et al. Chinese Food and Nutrition, 2016, 22(4): 32-34). It is a cancer preventive agent, an ulcer agent and a life extension agent. However, the human body cannot synthesize carotenoids, mainly from vegetables and fruits. The carotenoids in the sales market are mainly chemically synthesized, which is far from meeting the needs of modern consumers, and most companies currently use Carotenoid...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/89
CPCC12P23/00
Inventor 凌善锋韩佳祥蔡刘新张赢
Owner JINGCHU UNIV OF TECH
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