Scenedesmus obliquus producing self-flocculation substance, and application of the same in microalgae harvesting

A technology of Scenedesmus obliquus and self-flocculation, which is applied in the field of microbial applications, can solve the problems of the filtration method depending on the algae cell size, environmental toxicity, and high cost of the air flotation method, and achieve good pH stability and thermal stability.

Active Publication Date: 2013-02-27
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the centrifugation method consumes a lot of energy and costs high; the filtration method depends on the size of the algae cells, and is not suitable for large-scale microalgae collection; and the air flotation method is expensive and complicated to operate; in contrast, chemical flocculation can achieve a better microalgae collection effect , but the addition of a large amount of metal ions and refractory polymers undoubtedly increases the difficulty of downstream processing and has certain toxicity to the environment

Method used

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  • Scenedesmus obliquus producing self-flocculation substance, and application of the same in microalgae harvesting
  • Scenedesmus obliquus producing self-flocculation substance, and application of the same in microalgae harvesting
  • Scenedesmus obliquus producing self-flocculation substance, and application of the same in microalgae harvesting

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Experimental program
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Embodiment 1

[0032] Embodiment 1: The cultivation of the self-flocculating Scenedesmus obliquus of the present invention.

[0033] The self-flocculating Scenedesmus obliquus of the present invention is derived from the freshwater body in southern Taiwan, and the most suitable culture conditions are as follows: the pH of the initial medium is 6.0-6.2; the initial concentration is about 0.05-0.1 at O.D.685nm; the culture temperature is 28°C; the light intensity 60 μmol m -2 s -1 ; Photoperiod: 16h light culture, 8h dark culture. When cultured in the optimized DM liquid medium for 4-5 days, the algal cells are mostly in an aggregated state, and the size of the aggregates can be seen with the naked eye. After the 13-day culture medium is left still overnight, the algal cells aggregate together and settle into the Erlenmeyer flask At the bottom, the supernatant is clearly visible. Under the electron microscope, it was found that there was a membrane-like substance outside the algae cells tha...

Embodiment 2

[0033] The self-flocculating Scenedesmus obliquus of the present invention is derived from the freshwater body in southern Taiwan, and the most suitable culture conditions are as follows: the pH of the initial medium is 6.0-6.2; the initial concentration is about 0.05-0.1 at O.D.685nm; the culture temperature is 28°C; the light intensity 60 μmol m -2 s -1 ; Photoperiod: 16h light culture, 8h dark culture. When cultured in the optimized DM liquid medium for 4-5 days, the algal cells are mostly in an aggregated state, and the size of the aggregates can be seen with the naked eye. After the 13-day culture medium is left still overnight, the algal cells aggregate together and settle into the Erlenmeyer flask At the bottom, the supernatant is clearly visible. Under the electron microscope, it was found that there was a membrane-like substance outside the algae cells that wrapped the cells and adhered together ( figure 1) . Example 2: Genome extracted from Scenedesmus obliquus ...

Embodiment 3

[0047] Embodiment 3: the growth situation and the determination of biomass content of self-flocculation Scenedesmus oblique

[0048] The self-flocculating Scenedesmus obliquus was inoculated in the optimized DM liquid medium. After 5-13 days of culture, the algae grew rapidly, the number of algae cells increased rapidly, and the nitrogen content decreased significantly. The pH did not change much during the whole culture process, and the highest value was not More than 8, and no inorganic precipitates ( figure 2 ). After 16 days of cultivation, the protein content in the self-flocculating algae biomass can reach 28.4±1.3% of the dry weight, carbohydrates account for 25.3±1.0%, lipids account for 22.9±1.1%, and chlorophyll is as high as 13.0±0.6%, indicating that the algal strains of the present invention have Wide application prospects.

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Abstract

The invention relates to a strain of Scenedesmus obliquus producing a self-flocculation substance, and an application of the Scenedesmus obliquus in microalgae harvesting, and belongs to the technical field of microbial applications. The strain has a classification name of Scenedesmus obliquus and a preservation number of CGMCC No.6567, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 14, 2012, wherein the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing. With a flocculation substance produced by the Scenedesmus obliquus AS-6-1, non-flocculation strains such as freshwater scenedesmus FSP-3, freshwater chlorella CNW-11 and seawater chlorella DUT01 can be subjected to rapid flocculation harvesting so as to provide broad application prospects in microalgae harvesting. The flocculation substance is separated and purified from the self-flocculation Scenedesmus obliquus AS-6-1 cell wall, and is a water-soluble polysaccharide, wherein the non-flocculation scenedesmus FSP-3 is treated with 0.6 mg/L of the flocculation substance, and the flocculation efficiency can reach 80%; and the most suitable pH value is 7.0, the most suitable temperature is 30 DEG C, the flocculating activity does not require metal ions, and characteristics of time saving, efficiency, good pH value stability and good thermal stability are provided.

Description

technical field [0001] The invention relates to a Scenedesmus obliquus producing self-flocculating substances and its application in microalgae harvesting, which belongs to the technical field of microbial application. Background technique [0002] In recent years, with the rapid growth of the global economy, the consumption of fossil energy such as oil and coal has risen sharply, and the crisis of shortage of fossil energy is imminent, and attention to renewable energy such as biomass energy has gradually become a hot spot. Among the many non-food biomass, algae have wide distribution, strong environmental adaptability, high photosynthetic efficiency (the utilization rate of solar energy can reach 3-8%), short growth cycle (generally 1-10 days), oily With the characteristics of high oil content (oil content can reach 40-86% of dry weight), high yield (58,700L of oil per hectare per year, which is 1-2 times that of other oil-producing crops), the use of algae to produce biof...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 赵心清郭锁莲白凤武张嘉修
Owner DALIAN UNIV OF TECH
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